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YEAST:NCPR
Contents
| Species (Taxon ID) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). (559292) | |
| Gene Name(s) | NCP1 (ECO:0000255 with HAMAP-Rule:MF_03212) (synonyms: CPR1, NCPR1, PRD1) | |
| Protein Name(s) | NADPH--cytochrome P450 reductase (ECO:0000255 with HAMAP-Rule:MF_03212)
CPR (ECO:0000255 with HAMAP-Rule:MF_03212) P450R (ECO:0000255 with HAMAP-Rule:MF_03212) | |
| External Links | ||
| UniProt | P16603 | |
| EMBL | D13788 U00062 AY693091 BK006934 | |
| PIR | S46735 | |
| RefSeq | NP_011908.1 | |
| PDB | 2BF4 2BN4 2BPO 3FJO | |
| PDBsum | 2BF4 2BN4 2BPO 3FJO | |
| ProteinModelPortal | P16603 | |
| SMR | P16603 | |
| BioGrid | 36474 | |
| DIP | DIP-8294N | |
| IntAct | P16603 | |
| MINT | MINT-2782506 | |
| iPTMnet | P16603 | |
| MaxQB | P16603 | |
| PeptideAtlas | P16603 | |
| EnsemblFungi | [example_ID YHR042W] | |
| GeneID | 856438 | |
| KEGG | sce:YHR042W | |
| EuPathDB | FungiDB:YHR042W | |
| SGD | S000001084 | |
| GeneTree | ENSGT00620000087711 | |
| HOGENOM | HOG000282027 | |
| InParanoid | P16603 | |
| KO | K00327 | |
| OMA | VPIYVRK | |
| OrthoDB | EOG744TKC | |
| BioCyc | YEAST:YHR042W-MONOMER | |
| BRENDA | 1.6.2.4 | |
| Reactome | R-SCE-1474151 R-SCE-203615 R-SCE-5218920 R-SCE-5578775 | |
| EvolutionaryTrace | P16603 | |
| NextBio | 982042 | |
| PRO | PR:P16603 | |
| Proteomes | UP000002311 | |
| GO | GO:0005789 GO:0016021 GO:0005741 GO:0005886 GO:0009055 GO:0010181 GO:0005506 GO:0003958 GO:0006696 | |
| Gene3D | 1.20.990.10 3.40.50.360 | |
| HAMAP | MF_03212 | |
| InterPro | IPR003097 IPR017927 IPR001094 IPR008254 IPR001709 IPR029039 IPR023173 IPR001433 IPR023208 IPR017938 | |
| Pfam | PF00667 PF00258 PF00175 | |
| PIRSF | PIRSF000208 | |
| PRINTS | PR00369 PR00371 | |
| SUPFAM | SSF52218 SSF63380 | |
| PROSITE | PS51384 PS50902 | |
Annotations
| Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|---|
| GO:0009055 |
electron carrier activity |
other:GO_REF:0000100 |
ECO:0000247 |
|
F |
The transfer annotation HHpred hits had an E-value of 2.1E-24, a similarity of 99.9%, and a query coverage of 75.25% which serves as the evidence. |
complete | |||
| GO:0003959 |
NADPH dehydrogenase activity |
ECO:0000314 |
F |
The author cloned and overexpressed Ncp1 and monitored the reduction of Dph3 by Dph3's absorbance at 488nm. The author found that Ncp1 reduced Dph3 after the addition of NADPH to the reaction tube (Supplementary figure 5C). |
complete | |||||
|
part_of |
GO:0005741 |
mitochondrial outer membrane |
ECO:0007005 |
high throughput direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005739 |
mitochondrion |
ECO:0007005 |
high throughput direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005739 |
mitochondrion |
ECO:0007005 |
high throughput direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005739 |
mitochondrion |
ECO:0007005 |
high throughput direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
enables |
GO:0009055 |
electron transfer activity |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0006696 |
ergosterol biosynthetic process |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0003958 |
NADPH-hemoprotein reductase activity |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
has_direct_input:(UniProtKB:P10614) |
Seeded From UniProt |
complete | ||
|
enables |
GO:0050660 |
flavin adenine dinucleotide binding |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
EcoGene:EG10191 |
F |
Seeded From UniProt |
complete | ||
|
enables |
GO:0016491 |
oxidoreductase activity |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
MGI:MGI:97744 |
F |
Seeded From UniProt |
complete | ||
|
enables |
GO:0010181 |
FMN binding |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
EcoGene:EG10191 |
F |
Seeded From UniProt |
complete | ||
|
part_of |
GO:0005829 |
cytosol |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
PANTHER:PTN000453956 |
C |
Seeded From UniProt |
complete | ||
|
part_of |
GO:0005829 |
cytosol |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
PANTHER:PTN000453765 |
C |
Seeded From UniProt |
complete | ||
|
enables |
GO:0003958 |
NADPH-hemoprotein reductase activity |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
PANTHER:PTN000453763 |
F |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0022900 |
electron transport chain |
ECO:0000364 |
evidence based on logical inference from manual annotation used in automatic assertion |
GO:0009055 |
P |
Seeded From UniProt |
complete | ||
|
enables |
GO:0003958 |
NADPH-hemoprotein reductase activity |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0010181 |
FMN binding |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0016491 |
oxidoreductase activity |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
InterPro:IPR001433 |
F |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0055114 |
oxidation-reduction process |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
InterPro:IPR001094 |
P |
Seeded From UniProt |
complete | ||
|
enables |
GO:0003958 |
NADPH-hemoprotein reductase activity |
ECO:0000501 |
evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0050660 |
flavin adenine dinucleotide binding |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
UniRule:UR000239476 |
F |
Seeded From UniProt |
complete | ||
|
enables |
GO:0050661 |
NADP binding |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
UniRule:UR000239476 |
F |
Seeded From UniProt |
complete | ||
|
part_of |
GO:0005739 |
mitochondrion |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
UniRule:UR000239476 |
C |
Seeded From UniProt |
complete | ||
|
part_of |
GO:0005789 |
endoplasmic reticulum membrane |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
UniRule:UR000239476 |
C |
Seeded From UniProt |
complete | ||
|
part_of |
GO:0005737 |
cytoplasm |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
UniRule:UR000239476 |
C |
Seeded From UniProt |
complete | ||
|
part_of |
GO:0005886 |
plasma membrane |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
UniRule:UR000239476 |
C |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0006696 |
ergosterol biosynthetic process |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
UniRule:UR000239476 |
P |
Seeded From UniProt |
complete | ||
|
enables |
GO:0003958 |
NADPH-hemoprotein reductase activity |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
UniRule:UR000239476 |
F |
Seeded From UniProt |
complete | ||
|
enables |
GO:0010181 |
FMN binding |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
UniRule:UR000239476 |
F |
Seeded From UniProt |
complete | ||
|
part_of |
GO:0005741 |
mitochondrial outer membrane |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005783 |
endoplasmic reticulum |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
enables |
GO:0016491 |
oxidoreductase activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0016021 |
integral component of membrane |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0055114 |
oxidation-reduction process |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0006629 |
lipid metabolic process |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0016020 |
membrane |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005886 |
plasma membrane |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0008202 |
steroid metabolic process |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005739 |
mitochondrion |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0016126 |
sterol biosynthetic process |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0006694 |
steroid biosynthetic process |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005789 |
endoplasmic reticulum membrane |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ Helms, LR et al. (1990) Identification, sequence determination, and expression of the flavodoxin gene from Desulfovibrio salexigens. Biochem. Biophys. Res. Commun. 168 809-17 PubMed GONUTS page
- ↑ Lin, Z et al. (2016) Cbr1 is a Dph3 reductase required for the tRNA wobble uridine modification. Nat. Chem. Biol. PubMed GONUTS page
- ↑ Zahedi, RP et al. (2006) Proteomic analysis of the yeast mitochondrial outer membrane reveals accumulation of a subclass of preproteins. Mol. Biol. Cell 17 1436-50 PubMed GONUTS page
- ↑ Reinders, J et al. (2006) Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics. J. Proteome Res. 5 1543-54 PubMed GONUTS page
- ↑ Renvoisé, M et al. (2014) Quantitative variations of the mitochondrial proteome and phosphoproteome during fermentative and respiratory growth in Saccharomyces cerevisiae. J Proteomics 106 140-50 PubMed GONUTS page
- ↑ Sickmann, A et al. (2003) The proteome of Saccharomyces cerevisiae mitochondria. Proc. Natl. Acad. Sci. U.S.A. 100 13207-12 PubMed GONUTS page
- ↑ Lamb, DC et al. (2001) Activities and kinetic mechanisms of native and soluble NADPH-cytochrome P450 reductase. Biochem. Biophys. Res. Commun. 286 48-54 PubMed GONUTS page
- ↑ Venkateswarlu, K et al. (1998) The N-terminal membrane domain of yeast NADPH-cytochrome P450 (CYP) oxidoreductase is not required for catalytic activity in sterol biosynthesis or in reconstitution of CYP activity. J. Biol. Chem. 273 4492-6 PubMed GONUTS page
- ↑ Venkateswarlu, K et al. (1997) Characterization of Saccharomyces cerevisiae CYP51 and a CYP51 fusion protein with NADPH cytochrome P-450 oxidoreductase expressed in Escherichia coli. Antimicrob. Agents Chemother. 41 776-80 PubMed GONUTS page
- ↑ 10.0 10.1 10.2 10.3 10.4 10.5 Gaudet, P et al. (2011) Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Brief. Bioinformatics 12 449-62 PubMed GONUTS page
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