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YEAST:GCN4

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Species (Taxon ID) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). (559292)
Gene Name(s) GCN4 (synonyms: AAS3, ARG9)
Protein Name(s) General control protein GCN4

Amino acid biosynthesis regulatory protein

External Links
UniProt P03069
EMBL K02205
K02649
AJ585686
AJ585687
AJ585688
AJ585689
AJ585690
AJ585691
AJ585692
AJ585693
AJ585694
AJ585695
AJ585696
AJ585697
AJ585698
AJ585699
AJ585700
AJ585701
AJ585702
AJ585703
AJ585704
AF416613
U18530
BK006939
PIR A03605
RefSeq NP_010907.3
PDB 1CE9
1DGC
1ENV
1FAV
1FMH
1GCL
1GCM
1GK6
1GZL
1IHQ
1IJ0
1IJ1
1IJ2
1IJ3
1KQL
1LD4
1LLM
1NKN
1PIQ
1RB4
1RB5
1RB6
1SWI
1TMZ
1UNT
1UNU
1UNV
1UNW
1UNX
1UNY
1UNZ
1UO0
1UO1
1UO2
1UO3
1UO4
1UO5
1W5G
1W5H
1W5I
1W5J
1W5K
1W5L
1YSA
1ZII
1ZIJ
1ZIK
1ZIL
1ZIM
1ZTA
2AHP
2B1F
2B22
2BNI
2CCE
2CCF
2CCN
2D3E
2DGC
2EFR
2EFS
2G9J
2HY6
2IPZ
2K8X
2LPB
2NRN
2O7H
2OVN
2WG5
2WG6
2WPY
2WPZ
2WQ0
2WQ1
2WQ2
2WQ3
2YNY
2YNZ
2YO0
2YO1
2YO2
2YO3
2Z5H
2Z5I
2ZTA
3AZD
3BAS
3BAT
3CK4
3CRP
3G9R
3GJP
3I1G
3I5C
3K7Z
3M48
3P8M
3ZMF
4C46
4DMD
4G2K
4HU5
4HU6
4NIZ
4NJ0
4NJ1
4NJ2
4TL1
PDBsum 1CE9
1DGC
1ENV
1FAV
1FMH
1GCL
1GCM
1GK6
1GZL
1IHQ
1IJ0
1IJ1
1IJ2
1IJ3
1KQL
1LD4
1LLM
1NKN
1PIQ
1RB4
1RB5
1RB6
1SWI
1TMZ
1UNT
1UNU
1UNV
1UNW
1UNX
1UNY
1UNZ
1UO0
1UO1
1UO2
1UO3
1UO4
1UO5
1W5G
1W5H
1W5I
1W5J
1W5K
1W5L
1YSA
1ZII
1ZIJ
1ZIK
1ZIL
1ZIM
1ZTA
2AHP
2B1F
2B22
2BNI
2CCE
2CCF
2CCN
2D3E
2DGC
2EFR
2EFS
2G9J
2HY6
2IPZ
2K8X
2LPB
2NRN
2O7H
2OVN
2WG5
2WG6
2WPY
2WPZ
2WQ0
2WQ1
2WQ2
2WQ3
2YNY
2YNZ
2YO0
2YO1
2YO2
2YO3
2Z5H
2Z5I
2ZTA
3AZD
3BAS
3BAT
3CK4
3CRP
3G9R
3GJP
3I1G
3I5C
3K7Z
3M48
3P8M
3ZMF
4C46
4DMD
4G2K
4HU5
4HU6
4NIZ
4NJ0
4NJ1
4NJ2
4TL1
DisProt DP00083
ProteinModelPortal P03069
SMR P03069
BioGrid 36723
DIP DIP-591N
IntAct P03069
MINT MINT-395967
MaxQB P03069
PaxDb P03069
EnsemblFungi [example_ID YEL009C]
GeneID 856709
KEGG sce:YEL009C
CYGD YEL009c
SGD S000000735
eggNOG NOG329891
InParanoid P03069
KO K09464
OMA RKLQRMN
OrthoDB EOG75QRD1
BioCyc YEAST:G3O-30137-MONOMER
EvolutionaryTrace P03069
NextBio 982781
Proteomes UP000002311
Genevestigator P03069
GO GO:0005634
GO:0003682
GO:0042802
GO:0001191
GO:0001190
GO:0001135
GO:0043565
GO:0003700
GO:0001084
GO:0008652
GO:0010691
GO:0000122
GO:0001080
GO:0045899
GO:0060261
GO:0006366
InterPro IPR004827
Pfam PF00170
SMART SM00338
PROSITE PS50217
PS00036

Annotations

Qualifier GO ID GO term name Reference ECO ID ECO term name with/from Aspect Extension Notes Status
GO:0004672

protein kinase activity

PMID:21919885[1]

ECO:0000315

F

fig.2

complete

enables

GO:0043565

sequence-specific DNA binding

PMID:19158363[2]

ECO:0007005

high throughput direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

involved_in

GO:0010508

positive regulation of autophagy

PMID:26649943[3]

ECO:0000315

mutant phenotype evidence used in manual assertion

P

has_input:(UniProtKB:P38182)|has_input:(UniProtKB:P11491)

Seeded From UniProt

complete

involved_in

GO:0036278

positive regulation of transcription from RNA polymerase II promoter in response to nitrogen starvation

PMID:26649943[3]

ECO:0000315

mutant phenotype evidence used in manual assertion

P

regulates_transcription_of:(SGD:S000003148)

Seeded From UniProt

complete

enables

GO:0043621

protein self-association

PMID:25733613[4]

ECO:0000353

physical interaction evidence used in manual assertion

UniProtKB:P03069

F

Seeded From UniProt

complete

involved_in

GO:1903833

positive regulation of cellular response to amino acid starvation

PMID:6351059[5]

ECO:0000316

genetic interaction evidence used in manual assertion

UniProtKB:P09032

P

Seeded From UniProt

complete

involved_in

GO:1903833

positive regulation of cellular response to amino acid starvation

PMID:6351059[5]

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:0060261

positive regulation of transcription initiation from RNA polymerase II promoter

PMID:1425591[6]

ECO:0000316

genetic interaction evidence used in manual assertion

SGD:S000003315

P

Seeded From UniProt

complete

involved_in

GO:0060261

positive regulation of transcription initiation from RNA polymerase II promoter

PMID:1425591[6]

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:0045899

positive regulation of RNA polymerase II transcriptional preinitiation complex assembly

PMID:15121833[7]

ECO:0000316

genetic interaction evidence used in manual assertion

SGD:S000000189
SGD:S000000285
SGD:S000005335
SGD:S000005508
SGD:S000006175

P

Seeded From UniProt

complete

involved_in

GO:0045899

positive regulation of RNA polymerase II transcriptional preinitiation complex assembly

PMID:15121833[7]

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:0045899

positive regulation of RNA polymerase II transcriptional preinitiation complex assembly

PMID:18794364[8]

ECO:0000314

direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

enables

GO:0043565

sequence-specific DNA binding

PMID:3907851[9]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

enables

GO:0043565

sequence-specific DNA binding

PMID:3464968[10]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

enables

GO:0043565

sequence-specific DNA binding

PMID:2204805[11]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

enables

GO:0043565

sequence-specific DNA binding

PMID:3530496[12]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

enables

GO:0043565

sequence-specific DNA binding

PMID:3290651[13]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

involved_in

GO:0010691

negative regulation of ribosomal protein gene transcription from RNA polymerase II promoter in response to nutrient levels

PMID:21183953[14]

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

part_of

GO:0005634

nucleus

PMID:14648200[15]

ECO:0000314

direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

part_of

GO:0005634

nucleus

PMID:12455686[16]

ECO:0000314

direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

enables

GO:0003700

DNA-binding transcription factor activity

PMID:16581788[17]

ECO:0000315

mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete

enables

GO:0003700

DNA-binding transcription factor activity

PMID:16581788[17]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

enables

GO:0003682

chromatin binding

PMID:18794364[8]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

enables

GO:0001085

RNA polymerase II transcription factor binding

PMID:21183953[14]

ECO:0000353

physical interaction evidence used in manual assertion

SGD:S000005160

F

Seeded From UniProt

complete

enables

GO:0001135

RNA polymerase II transcription regulator recruiting activity

PMID:19940160[18]

ECO:0000353

physical interaction evidence used in manual assertion

SGD:S000005411

F

Seeded From UniProt

complete

enables

GO:0001135

RNA polymerase II transcription regulator recruiting activity

PMID:16581788[17]

ECO:0000315

mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete

enables

GO:0001135

RNA polymerase II transcription regulator recruiting activity

PMID:19940160[18]

ECO:0000315

mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete

enables

GO:0001135

RNA polymerase II transcription regulator recruiting activity

PMID:19940160[18]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

enables

GO:0001085

RNA polymerase II transcription factor binding

PMID:20308326[19]

ECO:0000353

physical interaction evidence used in manual assertion

SGD:S000002552

F

Seeded From UniProt

complete

involved_in

GO:0001080

nitrogen catabolite activation of transcription from RNA polymerase II promoter

PMID:21148207[20]

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:0000122

negative regulation of transcription by RNA polymerase II

PMID:15590823[21]

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:0000122

negative regulation of transcription by RNA polymerase II

PMID:26979516[22]

ECO:0000315

mutant phenotype evidence used in manual assertion

P

regulates_transcription_of:(SGD:S000000839)

Seeded From UniProt

complete

involved_in

GO:1903833

positive regulation of cellular response to amino acid starvation

PMID:21873635[23]

ECO:0000318

biological aspect of ancestor evidence used in manual assertion

PANTHER:PTN000894808
SGD:S000000735

P

Seeded From UniProt

complete

involved_in

GO:0045944

positive regulation of transcription by RNA polymerase II

PMID:21873635[23]

ECO:0000318

biological aspect of ancestor evidence used in manual assertion

FB:FBgn0001291
MGI:MGI:96646
MGI:MGI:96647
MGI:MGI:96648
PANTHER:PTN000894784
RGD:2943
UniProtKB:A0A1D8PIL5
UniProtKB:P05412
UniProtKB:P17275
UniProtKB:P17535

P

Seeded From UniProt

complete

involved_in

GO:0010033

response to organic substance

PMID:21873635[23]

ECO:0000318

biological aspect of ancestor evidence used in manual assertion

PANTHER:PTN000894784
RGD:2943

P

Seeded From UniProt

complete

part_of

GO:0005667

transcription factor complex

PMID:21873635[23]

ECO:0000318

biological aspect of ancestor evidence used in manual assertion

FB:FBgn0001291
MGI:MGI:96646
PANTHER:PTN000894784
ZFIN:ZDB-GENE-030131-7859

C

Seeded From UniProt

complete

part_of

GO:0005654

nucleoplasm

PMID:21873635[23]

ECO:0000318

biological aspect of ancestor evidence used in manual assertion

PANTHER:PTN000894784
UniProtKB:P05412
UniProtKB:P17275

C

Seeded From UniProt

complete

involved_in

GO:0001080

nitrogen catabolite activation of transcription from RNA polymerase II promoter

PMID:21873635[23]

ECO:0000318

biological aspect of ancestor evidence used in manual assertion

PANTHER:PTN000894808
SGD:S000000735

P

Seeded From UniProt

complete

enables

GO:0000981

DNA-binding transcription factor activity, RNA polymerase II-specific

PMID:21873635[23]

ECO:0000318

biological aspect of ancestor evidence used in manual assertion

FB:FBgn0001291
PANTHER:PTN000894784

F

Seeded From UniProt

complete

enables

GO:0000978

RNA polymerase II proximal promoter sequence-specific DNA binding

PMID:21873635[23]

ECO:0000318

biological aspect of ancestor evidence used in manual assertion

MGI:MGI:96647
MGI:MGI:96648
PANTHER:PTN000894784

F

Seeded From UniProt

complete

part_of

GO:0000790

nuclear chromatin

PMID:21873635[23]

ECO:0000318

biological aspect of ancestor evidence used in manual assertion

PANTHER:PTN000894784
RGD:2943

C

Seeded From UniProt

complete

involved_in

GO:0000122

negative regulation of transcription by RNA polymerase II

PMID:21873635[23]

ECO:0000318

biological aspect of ancestor evidence used in manual assertion

PANTHER:PTN000894784
SGD:S000000735

P

Seeded From UniProt

complete

enables

GO:0042802

identical protein binding

PMID:25733613[4]

ECO:0000353

physical interaction evidence used in manual assertion

UniProtKB:P03069

F

Seeded From UniProt

complete

enables

GO:0042802

identical protein binding

PMID:23348965[24]

ECO:0000353

physical interaction evidence used in manual assertion

UniProtKB:P03069

F

Seeded From UniProt

complete

enables

GO:0042802

identical protein binding

PMID:22157749[25]

ECO:0000353

physical interaction evidence used in manual assertion

UniProtKB:P03069

F

Seeded From UniProt

complete

enables

GO:0042802

identical protein binding

PMID:21474779[26]

ECO:0000353

physical interaction evidence used in manual assertion

UniProtKB:P03069

F

Seeded From UniProt

complete

enables

GO:0042802

identical protein binding

PMID:21045134[27]

ECO:0000353

physical interaction evidence used in manual assertion

UniProtKB:P03069

F

Seeded From UniProt

complete

enables

GO:0042802

identical protein binding

PMID:16844784[28]

ECO:0000353

physical interaction evidence used in manual assertion

UniProtKB:P03069

F

Seeded From UniProt

complete

enables

GO:0042802

identical protein binding

PMID:16472744[29]

ECO:0000353

physical interaction evidence used in manual assertion

UniProtKB:P03069

F

Seeded From UniProt

complete

enables

GO:0042802

identical protein binding

PMID:16402857[30]

ECO:0000353

physical interaction evidence used in manual assertion

UniProtKB:P03069

F

Seeded From UniProt

complete

involved_in

GO:0051123

RNA polymerase II preinitiation complex assembly

GO_REF:0000108

ECO:0000364

evidence based on logical inference from manual annotation used in automatic assertion

GO:0001135

P

Seeded From UniProt

complete

involved_in

GO:0051123

RNA polymerase II preinitiation complex assembly

GO_REF:0000108

ECO:0000364

evidence based on logical inference from manual annotation used in automatic assertion

GO:0001135

P

Seeded From UniProt

complete

involved_in

GO:0051123

RNA polymerase II preinitiation complex assembly

GO_REF:0000108

ECO:0000364

evidence based on logical inference from manual annotation used in automatic assertion

GO:0001135

P

Seeded From UniProt

complete

involved_in

GO:0051123

RNA polymerase II preinitiation complex assembly

GO_REF:0000108

ECO:0000364

evidence based on logical inference from manual annotation used in automatic assertion

GO:0001135

P

Seeded From UniProt

complete

enables

GO:0003700

DNA-binding transcription factor activity

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR004827

F

Seeded From UniProt

complete

involved_in

GO:0006355

regulation of transcription, DNA-templated

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR004827

P

Seeded From UniProt

complete

enables

GO:0003677

DNA binding

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0238

F

Seeded From UniProt

complete

part_of

GO:0005634

nucleus

GO_REF:0000037
GO_REF:0000039

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0539
UniProtKB-SubCell:SL-0191

C

Seeded From UniProt

complete

involved_in

GO:0008652

cellular amino acid biosynthetic process

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0028

P

Seeded From UniProt

complete

Notes

References

See Help:References for how to manage references in GONUTS.

  1. Hueso, G et al. (2012) A novel role for protein kinase Gcn2 in yeast tolerance to intracellular acid stress. Biochem. J. 441 255-64 PubMed GONUTS page
  2. Zhu, C et al. (2009) High-resolution DNA-binding specificity analysis of yeast transcription factors. Genome Res. 19 556-66 PubMed GONUTS page
  3. 3.0 3.1 Bernard, A et al. (2015) A large-scale analysis of autophagy-related gene expression identifies new regulators of autophagy. Autophagy 11 2114-2122 PubMed GONUTS page
  4. 4.0 4.1 Guidolin, LS et al. (2015) Interaction network and localization of Brucella abortus membrane proteins involved in the synthesis, transport, and succinylation of cyclic β-1,2-glucans. J. Bacteriol. 197 1640-8 PubMed GONUTS page
  5. 5.0 5.1 Hinnebusch, AG & Fink, GR (1983) Positive regulation in the general amino acid control of Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. U.S.A. 80 5374-8 PubMed GONUTS page
  6. 6.0 6.1 Mösch, HU et al. (1992) Sequence-specific initiator elements focus initiation of transcription to distinct sites in the yeast TRP4 promoter. EMBO J. 11 4583-90 PubMed GONUTS page
  7. 7.0 7.1 Qiu, H et al. (2004) An array of coactivators is required for optimal recruitment of TATA binding protein and RNA polymerase II by promoter-bound Gcn4p. Mol. Cell. Biol. 24 4104-17 PubMed GONUTS page
  8. 8.0 8.1 Zhang, F et al. (2008) Disrupting vesicular trafficking at the endosome attenuates transcriptional activation by Gcn4. Mol. Cell. Biol. 28 6796-818 PubMed GONUTS page
  9. Hope, IA & Struhl, K (1985) GCN4 protein, synthesized in vitro, binds HIS3 regulatory sequences: implications for general control of amino acid biosynthetic genes in yeast. Cell 43 177-88 PubMed GONUTS page
  10. Arndt, K & Fink, GR (1986) GCN4 protein, a positive transcription factor in yeast, binds general control promoters at all 5' TGACTC 3' sequences. Proc. Natl. Acad. Sci. U.S.A. 83 8516-20 PubMed GONUTS page
  11. Sellers, JW et al. (1990) Mutations that define the optimal half-site for binding yeast GCN4 activator protein and identify an ATF/CREB-like repressor that recognizes similar DNA sites. Mol. Cell. Biol. 10 5077-86 PubMed GONUTS page
  12. Hope, IA & Struhl, K (1986) Functional dissection of a eukaryotic transcriptional activator protein, GCN4 of yeast. Cell 46 885-94 PubMed GONUTS page
  13. Roussou, I et al. (1988) Transcriptional-translational regulatory circuit in Saccharomyces cerevisiae which involves the GCN4 transcriptional activator and the GCN2 protein kinase. Mol. Cell. Biol. 8 2132-9 PubMed GONUTS page
  14. 14.0 14.1 Joo, YJ et al. (2011) Gcn4p-mediated transcriptional repression of ribosomal protein genes under amino-acid starvation. EMBO J. 30 859-72 PubMed GONUTS page
  15. Pries, R et al. (2004) Nuclear import of yeast Gcn4p requires karyopherins Srp1p and Kap95p. Mol. Genet. Genomics 271 257-66 PubMed GONUTS page
  16. Pries, R et al. (2002) Amino acid-dependent Gcn4p stability regulation occurs exclusively in the yeast nucleus. Eukaryotic Cell 1 663-72 PubMed GONUTS page
  17. 17.0 17.1 17.2 Qiu, H et al. (2006) The Spt4p subunit of yeast DSIF stimulates association of the Paf1 complex with elongating RNA polymerase II. Mol. Cell. Biol. 26 3135-48 PubMed GONUTS page
  18. 18.0 18.1 18.2 Jedidi, I et al. (2010) Activator Gcn4 employs multiple segments of Med15/Gal11, including the KIX domain, to recruit mediator to target genes in vivo. J. Biol. Chem. 285 2438-55 PubMed GONUTS page
  19. Herbig, E et al. (2010) Mechanism of Mediator recruitment by tandem Gcn4 activation domains and three Gal11 activator-binding domains. Mol. Cell. Biol. 30 2376-90 PubMed GONUTS page
  20. Teixeira, MC et al. (2011) Yeast response and tolerance to polyamine toxicity involving the drug : H+ antiporter Qdr3 and the transcription factors Yap1 and Gcn4. Microbiology (Reading, Engl.) 157 945-56 PubMed GONUTS page
  21. Velasco, I et al. (2004) Saccharomyces cerevisiae Aqr1 is an internal-membrane transporter involved in excretion of amino acids. Eukaryotic Cell 3 1492-503 PubMed GONUTS page
  22. Yadav, KK & Rajasekharan, R (2016) The transcription factor GCN4 regulates PHM8 and alters triacylglycerol metabolism in Saccharomyces cerevisiae. Curr. Genet. 62 841-851 PubMed GONUTS page
  23. 23.0 23.1 23.2 23.3 23.4 23.5 23.6 23.7 23.8 23.9 Gaudet, P et al. (2011) Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Brief. Bioinformatics 12 449-62 PubMed GONUTS page
  24. Erce, MA et al. (2013) A conditional two-hybrid (C2H) system for the detection of protein-protein interactions that are mediated by post-translational modification. Proteomics 13 1059-64 PubMed GONUTS page
  25. Cisneros, DA et al. (2012) Minor pseudopilin self-assembly primes type II secretion pseudopilus elongation. EMBO J. 31 1041-53 PubMed GONUTS page
  26. Miot, M et al. (2011) Species-specific collaboration of heat shock proteins (Hsp) 70 and 100 in thermotolerance and protein disaggregation. Proc. Natl. Acad. Sci. U.S.A. 108 6915-20 PubMed GONUTS page
  27. Ciani, B et al. (2010) Molecular basis of coiled-coil oligomerization-state specificity. Proc. Natl. Acad. Sci. U.S.A. 107 19850-5 PubMed GONUTS page
  28. Singh, A et al. (2006) Dissecting virulence pathways of Mycobacterium tuberculosis through protein-protein association. Proc. Natl. Acad. Sci. U.S.A. 103 11346-51 PubMed GONUTS page
  29. Deng, Y et al. (2006) Antiparallel four-stranded coiled coil specified by a 3-3-1 hydrophobic heptad repeat. Structure 14 247-55 PubMed GONUTS page
  30. Benton, R et al. (2006) Atypical membrane topology and heteromeric function of Drosophila odorant receptors in vivo. PLoS Biol. 4 e20 PubMed GONUTS page