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User:Vishwanathk

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Phage Hunters Spring 2016

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
unacceptable9CAUD:J9PW752016-04-11 04:57:47 CDTGO:0004527 exonuclease activity (F)other:GO_REF:0000100ECO:0000247 sequence alignment evidence used in manual assertion

On Blast, the gene matches the putative exonuclease 2 protein and has 97% coverage and 60% identity to the sequence that was processed from Eyuki. This sequence also has an E-value of 1e-160.

challenge
acceptableBPT4:END72016-05-02 20:50:37 CDTGO:0004519 endonuclease activity (F)PMID:2269300ECO:0000314 direct assay evidence used in manual assertion

The enzyme endonuclease VII cleaves the DNA into smaller pieces. This article presents a figure (3a and 3b) that shows the enzyme activity of endonuclease VIIcl (cl = clone, Y-axis) versus the sedimentation centrifugation fraction (including inset with fraction for molecular masses of each marker protein used). The activity of the endnuclease VIIcl enzyme is shown to be in between the appropriate masses of each protein (monomer and aggregated forms) used in the sedimentation experiment.

challenge
unacceptable9CAUD:A0A0K2D0002016-05-06 01:26:55 CDTGO:0004146 dihydrofolate reductase activity (F)other:GO_REF:0000100ECO:0000247 sequence alignment evidence used in manual assertion

The blast data for this sequence from Eyuki matches the diydrofolate reductase gene on TsarBomba. This data shows an e-value of 7e-41, an identity match of 46%, and a coverage of 98%.

challenge
unacceptable9CAUD:A0A0K2D0H82016-05-06 05:22:54 CDTGO:0008233 peptidase activity (F)GO_REF:0000100ECO:0000247 sequence alignment evidence used in manual assertion

The blast data shows that there is a 95% coverage and a 58% identity to a prohead protease gene in Moonbeam. Also, the e-value is 3e-102.

challenge
unacceptableBPA51:Q379162016-05-06 05:42:49 CDTGO:0098027 virus tail, sheath (C)GO_REF:0000100ECO:0000247 sequence alignment evidence used in manual assertion

The blast data shows a 98% coverage and a 42% identity to a tail sheath protein precursor in Eyuki. Also, the e-value is 7e-142.

challenge

acceptable:1
unacceptable:4
requires_changes:0
flagged:0

Annotations challenged by Vishwanathk

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
unacceptableE63,
Team Blue A
LACCA:DYRGO:0050661 - NADP binding (F)PMID:6815179ECO:0000314 direct assay evidence used in manual assertionchallengeC: 2
unacceptableStankalwala,
Team T2
DANRE:E7F8P0GO:0006915 - apoptotic process (P)PMID:27050765ECO:0000314 direct assay evidence used in manual assertionchallengeC: 2
updatedbyinstructorBenjaminJones,
Team T7
BPT4:ALCGO:0039653 - suppression by virus of host transcription (P)PMID:2185231ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 1
updatedbyinstructorBenjaminJones,
Team T7
BPT4:MODAGO:0070213 - protein auto-ADP-ribosylation (P)PMID:15489438ECO:0000314 direct assay evidence used in manual assertionchallengeC: 1
updatedbyinstructorBenjaminJones,
Team T7
BPT4:ALTGO:0006471 - protein ADP-ribosylation (P)PMID:173540ECO:0000314 direct assay evidence used in manual assertionchallengeC: 1
updatedbyinstructorBenjaminJones,
Team T7
BPT4:TEV1GO:1990238 - double-stranded DNA endodeoxyribonuclease activity (F)PMID:2543665ECO:0000314 direct assay evidence used in manual assertionchallengeC: 1
updatedbyinstructorBenjaminJones,
Team T7
BPT4:PINGO:0030414 - peptidase inhibitor activity (F)PMID:2838455ECO:0000314 direct assay evidence used in manual assertionchallengeC: 1
updatedbyinstructorKetkiPatil,
Team T5
BPT5:EXO5GO:0019086 - late viral transcription (P)PMID:4357874ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 1

0 annotations fixed by Vishwanathk

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