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User:MckaelaAutumn19

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CACAO Fall 2018

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
unacceptable9CAUD:U5PW982018-09-11 14:05:52 CDTGO:0051260 protein homooligomerization (P)PMID:29298884ECO:0000314 direct assay evidence used in manual assertion

Figure 8 uses mass exclusion chromatography to suggest gp39/Dpo1/tail fiber protein in Acinetobacter phage petty forms a protein hexamer of identical monomers.

challenge
acceptable9CAUD:U5PVL22018-09-11 14:35:16 CDTGO:0019012 virion (C)PMID:29298884ECO:0000314 direct assay evidence used in manual assertion

Figure 5 suggests that Internal core protein/gp 38 is a cellular component of Acinetobacter phage Petty because mass spectrometry shows it is present in the phage.

challenge
acceptable9MONO:F4YH962018-09-18 14:00:29 CDTGO:0019068 virion assembly (P)PMID:29695428ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 4 suggests that in Henipavirus Hendra, HeV M/Matrix protein affects VLP (virus like particle) production efficiency because mutants of HeV M protein showed a large decrease in relative VLP levels.

challenge
acceptable9MONO:G5CPX12018-09-25 14:34:58 CDTGO:0046813 receptor-mediated virion attachment to host cell (P)PMID:26585033ECO:0000314 direct assay evidence used in manual assertion

Figure 2 shows the binding of sHeV G/attachment glycoprotein/HeV attachment protein from Heniparvirus Hendra with EFNB2/Fc protein using an ELISA plate.

challenge
unacceptablePRIBE:X2JJ722018-10-02 13:18:43 CDTGO:0006582 melanin metabolic process (P)PMID:25143047ECO:0000314 direct assay evidence used in manual assertion

Table 1 suggests that ASIP/agouti receptor plays a part in melanism in Prionailurus bengalensis because it shows the color phenotypes of different variants found in domestic cats, wild cats, and hybrids.

challenge
unacceptableCAEEL:Q9XX852018-10-02 14:07:41 CDTGO:0042048 olfactory behavior (P)PMID:30258230ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2 compares the chemotaxis in Caenorhabditis elegans between str-217/seven TM receptor wild type and mutants with a C->T mutation and a deletion to show that a mutation and shows a mutation leads to an increase in response compared to wild-type, supporting mutants are DEET resistant.

challenge
unacceptable9MONO:F4YH912018-10-02 14:32:56 CDTGO:0005198 structural molecule activity (F)PMID:26196500ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2 shows the comparison of the Western blots of Hendra virus nucleocapsid protein/HeV N/nucleocapsid wild type and a mutant in Henipavirus Hendra to show its presence within the virion.

challenge
acceptable9VIRU:Q27XI92018-10-30 13:50:22 CDTGO:0039615 T=1 icosahedral viral capsid (C)PMID:20375175ECO:0000314 direct assay evidence used in manual assertion

Figure 2 shows, with cyro-reconstruction that the viral protein 2/VLP 2 has an icosahedral capsid in human bocavirus virus like particles.

challenge
unacceptableBPT4:CAPSH2018-10-30 14:23:24 CDTPMID:15846365ECO:0000314 direct assay evidence used in manual assertion

Figure 5 shows the folding interaction of GroEL and gp23 within Enterobacteria phage T4/bacteriophage T4 using mass spectrometry.

challenge
acceptableBPT4:VG312018-10-30 14:35:56 CDTGO:0051085 chaperone cofactor-dependent protein refolding (P)PMID:15846365ECO:0000021 physical interaction evidence

Figure 2 shows the interaction of GroEL and Capsid assembly protein Gp31/gp31 within Enterobacteria phage T4/bacteriophage T4 only in the presence of ADP and Mg2+ using mass spectrometry

challenge
acceptableHBOC2:NP12018-11-13 13:49:42 CSTGO:0031564 transcription antitermination (P)PMID:26912614ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 5C shows a Northern Blot that compares VP mRNA in relation to regular NP1/Non-structural protein NP-1 and different mutants and knockout mutants, pCMVNS(NP*)CAP and pCMVNS*(NP*)Cap, in human bocavirus.

challenge
acceptableHBOC2:NP12018-11-13 13:55:41 CSTGO:0033120 positive regulation of RNA splicing (P)PMID:26912614ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 6D compares the VP mRNA levels of normally functioning non-structural protein NP-1/NP1 and that of a knockout mutant from the human bocavirus using a Northern blot and levels are seen to be decreased with the knockout mutant.

challenge
acceptableHENDH:FUS2018-11-13 14:22:16 CSTGO:0044228 host cell surface (C)PMID:19553334ECO:0000314 direct assay evidence used in manual assertion

Figure 3 is showing the trafficking rates of Hendra virus F/fusion glycoprotein F0/Hendra F0 as it makes its way to the surface of the cell in Henipavirus Hendra.

challenge
acceptableHENDH:GLYCP2018-11-13 14:25:41 CSTGO:0044228 host cell surface (C)PMID:19553334ECO:0000314 direct assay evidence used in manual assertion

Figure 3 is showing the trafficking rates of Hendra virus G/glycoprotein G/Hendra G as it makes its way to the surface of the cell in Henipavirus Hendra.

challenge
acceptableSENDZ:V2018-11-13 14:39:02 CSTGO:1900226 negative regulation of NLRP3 inflammasome complex assembly (P)PMID:30021903ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2 shows the inhibition of NLRP3 expression levels in cells infected with Sev V protein/Protein V from Sendai virus is present.

challenge
acceptableHUMAN:SYUA2018-11-15 15:33:02 CSTGO:0035543 positive regulation of SNARE complex assembly (P)PMID:20798282ECO:0000314 direct assay evidence used in manual assertion

Figure 1 shows the immunoprecipitation of α-Synuclein/α-S/alpha-Synuclein/Snca of Homo sapiens in transgenic Mus musculus with SNARE complexes immunoprecipitated with SNAP-25 to measure the activity.

challenge
unacceptableBPT4:RLIG2018-11-18 09:09:45 CSTGO:0003910 DNA ligase (ATP) activity (F)PMID:26500066ECO:0000314 direct assay evidence used in manual assertion

Figure 2 shows the catalysis results of T4 RNA ligase 1/T4 RNL1 in combination with varying levels of ATP in Enterobacteria phage T4.

challenge
unacceptableBPT4:RLIG2018-11-18 09:38:11 CSTGO:0003909 DNA ligase activity (F)PMID:21722378ECO:0000314 direct assay evidence used in manual assertion

Figure 2 shows the ligation products of 3'-blocked and 5'- adenylated DNA and T4 RNA ligase 1/T4 RNL1 from Enterobacteria phage T4.

challenge
unacceptableBPT4:RLIG22018-11-18 09:44:31 CSTGO:0003909 DNA ligase activity (F)PMID:21722378ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 7 compares the ligation products of T4 RNA ligase 2/T4 RNL2 and mutants of this protein in Enterobacteria phage T4.

challenge
acceptableBOTFB:A0A384JIP42018-11-18 10:41:25 CSTGO:0000719 photoreactive repair (P)PMID:28667107ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 3 compares the photoreactivation of BcCRY1 to BcCRY2, mutant BcCRY1, and mutant BcCRY2 in photorepair in Botryotinia fuckeliana (strain B05.10) (Noble rot fungus) (Botrytis cinerea) when exposed to UV light for different intervals.

challenge
acceptableBOTFB:A0A384JRT32018-11-18 10:50:53 CSTGO:0060258 negative regulation of filamentous growth (P)PMID:28667107ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 4 compares the mycelia growth of Botryotinia fuckeliana (strain B05.10) (Noble rot fungus) (Botrytis cinerea) wildtype, BcCRY2, and BcCRY2 mutant to show that BcCRY2 negatively regulates radial growth of the fungi.

challenge
acceptableBOTFB:A0A384JRT32018-11-18 10:59:18 CSTGO:0075308 conidium formation (P)PMID:28667107ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 5 shows that BcCRY2 negatively regulates conidiation in Botryotinia fuckeliana (strain B05.10) (Noble rot fungus) (Botrytis cinerea) by comparing the the conidation transcript levels and condination of wildtype, BcCRY2, and BcCRY2 mutant.

challenge
unacceptableHYPAT:O939632018-11-19 22:42:51 CSTGO:1904361 positive regulation of spore germination (P)PMID:17545314ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 4 compares the spore germination of Trichoderma atrovirid in response to light stimulus and exposure to UV radiation of PHR1/DNA photolyase and a mutant of the protein.

challenge
acceptableBPT4:MOTB2018-11-20 08:26:43 CSTGO:0003697 single-stranded DNA binding (F)PMID:29949907ECO:0000314 direct assay evidence used in manual assertion

Figure 3B shows that Transcription regulatory protein motB/MotB from Bacteriophage T4 binds to host ssDNA and copurifies when the host ssDNA is purified.

challenge
acceptableHYPAT:O939632018-11-20 11:59:50 CSTGO:0000719 photoreactive repair (P)PMID:17545314ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2 compares the survival rates of Trichoderma atroviride in relation to the phr1/DNA photolyase protein and its mutant when exposed to UV light and then either exposed to light or kept in the dark.

challenge
acceptableARATH:CRYD2018-11-20 12:24:49 CSTGO:0000719 photoreactive repair (P)PMID:19074258ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 5 compares the repair rates of Arabidopsis thaliana in relation to cry3/CRYD/Cryptochrome DASH/ chloroplastic/mitochondrial and its mutant when exposed to light in various amounts after UV treatment.

challenge
updatedbyinstructorASFB7:P102018-11-20 14:01:02 CSTGO:0039642 virion nucleoid (C)PMID:30185597ECO:0000314 direct assay evidence used in manual assertion

based on synthesizing figure 1, table 1, and immunogold in figure 3

challenge
unacceptableASF:Q5IZK22018-11-20 14:06:30 CSTGO:0039660 structural constituent of virion (F)PMID:30185597ECO:0000314 direct assay evidence used in manual assertion

Figure 1 uses a Western Blot to show P72/B646L CDS protein is a structural protein found in African Swine Fever Virus particles.

challenge
acceptableBPT4:MOTB2018-11-20 14:17:25 CSTGO:0003690 double-stranded DNA binding (F)PMID:29949907ECO:0000314 direct assay evidence used in manual assertion

Figure 3A shows that Transcription regulatory protein motB/MotB from Bacteriophage T4 binds to host dsDNA and copurifies when the host dsDNA is purified.

challenge

acceptable:18
unacceptable:10
requires_changes:0
flagged:0

Annotations challenged by MckaelaAutumn19

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
updatedbyinstructorMayurSST,
Team Donuts
HCVH:POLGGO:0019054 - modulation by virus of host cellular process (P)PMID:24390321ECO:0000314 direct assay evidence used in manual assertionchallengeC: 4

fixed by MayurSST
updatedbyinstructorMayurSST,
Team Donuts
HCVH:POLGGO:1903301 - positive regulation of hexokinase activity (P)PMID:24390321ECO:0000314 direct assay evidence used in manual assertionchallengeC: 4

fixed by MayurSST
unacceptableAshFlipp3,
Team Donuts2
CANLF:A0A0C5E1H5GO:1900119 - positive regulation of execution phase of apoptosis (P)PMID:25890299ECO:0000314 direct assay evidence used in manual assertionchallengeC: 1

0 annotations fixed by MckaelaAutumn19