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User:MayurSST

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CACAO Fall 2018

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptableDANRE:Q6K1982018-09-11 13:56:55 CDTGO:0007498 mesoderm development (P)PMID:15169890ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 6. p100 Zebra Fish

challenge
updatedbyinstructorHCVH:POLG2018-10-02 14:12:30 CDTGO:1903301 positive regulation of hexokinase activity (P)PMID:24390321ECO:0000314 direct assay evidence used in manual assertion

Figure 7 shows statistically significant increases in hexokinase activity due to NS5A. Hepatitis C NS5A was used on Human Hexokinase

challenge
updatedbyinstructorHCVH:POLG2018-10-02 14:11:56 CDTGO:0019054 modulation by virus of host process (P)PMID:24390321ECO:0000314 direct assay evidence used in manual assertion

Figures 6 and 7 show that Hepatitis C Virus NS5A affect Human Glycolysis.

challenge
unacceptableMOUSE:HXK42018-10-16 13:12:32 CDTGO:0032811 negative regulation of epinephrine secretion (P)PMID:30146176ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 4g shows that when GCK is knocked out in mice, plasma epinephrine levels rise significantly.

challenge
acceptableMOUSE:HXK42018-10-16 13:39:50 CDTGO:0004340 glucokinase activity (F)PMID:30146176ECO:0000315 mutant phenotype evidence used in manual assertion

Supplementary figures 1a and 1b show that GCK mutant mice show reduced glucose phosphorylating activity in the liver and pancreas.

challenge
unacceptableRAT:CNR12018-10-16 13:51:11 CDTGO:0014054 positive regulation of gamma-aminobutyric acid secretion (P)PMID:30217553ECO:0000314 direct assay evidence used in manual assertion

Figure 2 shows that when a CB1 agonist was infused in rats , GABA secretion increased whereas when a CB1 antagonist was introduced GABA secretion was not modified.

challenge
updatedbyinstructorRAT:CNR12018-10-16 13:57:49 CDTGO:0014063 negative regulation of serotonin secretion (P)PMID:30217553ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 3 shows that when a CB1 agonist is infused, 5-HT secretion decreased wheres when a CB1 antagonist is infused 5-HT secretion was unmodified compared to controls.

Cannabinoid receptor 1, and CB1

Rattus norvegicus (Rat).

challenge
updatedbyinstructorRAT:5HT1A2018-10-16 14:24:46 CDTGO:0014053 negative regulation of gamma-aminobutyric acid secretion (P)PMID:30217553ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 5 shows that when 5-HT 1a(8-OH-DPAT) is activated the up regulation of GABA secretion due to CB1 activation is blocked.

5-hydroxytryptamine receptor 1A, and 5-HT1A, Rattus norvegicus (Rat)

challenge
updatedbyinstructorRAT:5HT1B2018-10-16 14:29:47 CDTGO:0014053 regulation of gamma-aminobutyric acid secretion (P)PMID:30217553ECO:0000314 direct assay evidence used in manual assertion

Figure 6 shows that when 5-HT 1b (CP 93129) is activated the up regulation of GABA secretion due to CB1 activation is blocked. Rat

challenge
unacceptableRAT:RAB3A2018-11-13 13:03:06 CSTGO:0014059 regulation of dopamine secretion (P)PMID:28370453ECO:0000314 direct assay evidence used in manual assertion

Figure 2A and 2c show that there are concentration dependent effects of RAB3a on Dopamine release in culture medium. The RAB3a was taken from rats.

challenge
acceptableRAT:RAB3A2018-11-13 13:02:42 CSTGO:0014059 regulation of dopamine secretion (P)PMID:28370453ECO:0000314 direct assay evidence used in manual assertion

Figure 2A and 2c show that there are concentration dependent effects of RAB3a on Dopamine release in culture medium. The RAB3a was taken from rats.

challenge
acceptableRAT:SYT12018-11-13 13:27:27 CSTGO:0033603 dopamine secretion (P)PMID:28370453ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 4c shows that in the absence of RAB3a the dopamine content was significantly lower after the SYT1 knockdown when compared to figure 2c - without the SYT 1 knockdown. The proteins are taken from rats.

challenge
unacceptablePIG:LEP2018-11-13 14:27:19 CSTGO:1900534 palmitic acid catabolic process (P)PMID:14677856ECO:0000314 direct assay evidence used in manual assertion

Figure 4 shows that in pigs chronic exposure to porcine leptin increases the relative rate of palmitate oxidation. Submitting new go term request for 'Positive regulation of palmitic acid catabolic process'

challenge
acceptablePIG:LEP2018-11-13 14:35:35 CSTGO:0046627 glucose catabolic process (P)PMID:14677856ECO:0000314 direct assay evidence used in manual assertion

Figure 1 shows that acute treatment with porcine leptin reduced the rate of glucose oxidation in pig adipocyte cultures.

challenge
unacceptablePIG:LEP2018-11-13 18:02:42 CSTGO:0006007 glucose catabolic process (P)PMID:14677856ECO:0000314 direct assay evidence used in manual assertion

Figure 2 shows that chronic treatment with porcine leptin reduced the rate of glucose oxidation in pig adipocyte containing cultures

challenge
unacceptablePIG:LEP2018-11-13 18:04:53 CSTGO:0051055 negative regulation of lipid biosynthetic process (P)PMID:14677856ECO:0000314 direct assay evidence used in manual assertion

Figure 6 shows that chronic leptin treatment reduce the total glucose incorporation into lipids(lipid biosynthesis) vs controls in pig adipocyte containing cultures.

challenge
unacceptablePIG:LEP2018-11-13 19:46:32 CSTGO:1900533 palmitic acid metabolic process (P)PMID:14677856ECO:0000314 direct assay evidence used in manual assertion

Figure 7 shows that acute treatment with porcine leptin caused a decrease in palmitate esterification in pig adipocyte-containing primary cultures.

challenge
unacceptablePIG:LEP2018-11-13 19:48:08 CSTGO:0045717 negative regulation of fatty acid biosynthetic process (P)PMID:14677856ECO:0000314 direct assay evidence used in manual assertion

Figure 10 shows that chronic treatment with porcine leptin decreased fatty acid synthesis in a dose dependent manner in pig adipocyte containing primary cultures

challenge
acceptablePIG:INS2018-11-13 19:50:05 CSTGO:0045723 positive regulation of fatty acid biosynthetic process (P)PMID:14677856ECO:0000314 direct assay evidence used in manual assertion

Figures 9 and 10 show that treatment with insulin caused an increase in fatty acid synthesis in pig adipocyte-containing primary cultures.

challenge
unacceptablePIG:INS2018-11-13 19:51:31 CSTGO:0045723 positive regulation of fatty acid biosynthetic process (P)PMID:14677856ECO:0000314 direct assay evidence used in manual assertion

Figure 9 shows that treatment with insulin caused an increase in fatty acid synthesis in pig adipocyte-containing primary cultures.

challenge
unacceptablePIG:INS2018-11-13 19:53:23 CSTGO:0046889 positive regulation of lipid biosynthetic process (P)PMID:14677856ECO:0000314 direct assay evidence used in manual assertion

Figure 5 shows that insulin increased the glucose incorporation into lipid(lipid biosynthesis) vs controls in porcine(pig) adipocyte culture..

challenge
acceptablePIG:INS2018-11-13 19:54:42 CSTGO:0051006 positive regulation of lipoprotein lipase activity (P)PMID:14677856ECO:0000314 direct assay evidence used in manual assertion

Figure 11 shows that chronic exposure to insulin stimulated an increase lipoprotein lipase activity in Pig adipocyte containing primary cultures.

challenge
acceptablePIG:INS2018-11-17 17:33:14 CSTGO:0010907 glucose catabolic process (P)PMID:14677856ECO:0000314 direct assay evidence used in manual assertion

Figure 1 shows that insulin tended to increase glucose oxidation in pig adipocyte cultures. Requesting new term 'Positive regulation of glucose catabolic process'

challenge

acceptable:8
unacceptable:10
requires_changes:0
flagged:0

Annotations challenged by MayurSST

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
updatedbyinstructorMayurSST,
Team Donuts
HCVH:POLGGO:1903301 - positive regulation of hexokinase activity (P)PMID:24390321ECO:0000314 direct assay evidence used in manual assertionchallengeC: 4

fixed by MayurSST
updatedbyinstructorMayurSST,
Team Donuts
HCVH:POLGGO:0019054 - modulation by virus of host cellular process (P)PMID:24390321ECO:0000314 direct assay evidence used in manual assertionchallengeC: 4

fixed by MayurSST

2 annotations fixed by MayurSST