User:Marhuang

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
updatedbyinstructor	RAT:KCC2D	2013-04-24 13:18:37 CDT	GO:0004683 calmodulin-dependent protein kinase activity (F)	PMID:22514276	ECO:0000314 direct assay evidence used in manual assertion	Figure 1B. Immunoblot of HEK293 cells expressing Nav1.5 were exposed to rat CAMKIIdC showing phosphorylation of site on loop 1 of sodium channel.	challenge
acceptable	RAT:KCC2D	2013-04-26 10:43:03 CDT	GO:0004672 protein kinase activity (F)	PMID:22514276	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 5: Electrophysiology of channel with mutant phenotype T594A and S516A and also in comparison to drug AIP and WT phenotypes	challenge
requireschanges	RAT:KCC2D	2013-04-26 14:22:20 CDT	GO:0005954 calcium- and calmodulin-dependent protein kinase complex (C)	PMID:21677263	ECO:0000314 direct assay evidence used in manual assertion	Figure 3: Immunoblot shows CaMKIId forms complex and increases activity of sodium channel	challenge
requireschanges	RABIT:KCC2D	2013-04-26 14:38:48 CDT	GO:0004683 calmodulin-dependent protein kinase activity (F)	PMID:21835909	ECO:0000314 direct assay evidence used in manual assertion	Figure 6: Activation of CaMKII in rabbit cardiomyocytes using FRET	challenge
acceptable	CHICK:EPHB2	2013-04-26 15:36:15 CDT	GO:0021963 spinothalamic tract morphogenesis (P)	PMID:19004859	ECO:0000314 direct assay evidence used in manual assertion	Figure 4: Overexpression of EphB2 in neurogenic crest leads to ectopic DL migration	challenge
acceptable	MYXXD:Q1DDD5	2013-04-27 14:38:36 CDT	GO:0010468 regulation of gene expression (P)	PMID:16484184	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 6: Wildtype vs mutant run on SDS-PAGE gel	challenge
acceptable	HUMAN:TGM2	2013-05-23 13:24:40 CDT	GO:0031226 intrinsic to plasma membrane (C)	PMID:8105889	ECO:0000315 mutant phenotype evidence used in manual assertion	TGK becomes quickly membrane bound. When mutated, TGK mutant is not membrane bound. Figure 2 identifies the localization of native and mutant using fractionation and then immunoblotting of the immunoprecipitation.	challenge
updatedbyinstructor	?	2013-05-23 15:00:01 CDT	GO:0019867 outer membrane (Figure 2: Comparison to mutant xylella)	PMID:20378647	IMP: Inferred from Mutant Phenotype		challenge
acceptable	MYXXD:Q1DA50	2013-05-23 19:41:45 CDT	GO:0031155 regulation of fruiting body development (P)	PMID:22389486	ECO:0000315 mutant phenotype evidence used in manual assertion	"Surprisingly, development on CF agar was similar to that of the wild type, as the Δark mutant developed larger aggregates with slightly more defined edges than those for fruiting on TPM agar. The Δark mutant also developed a web-like appearance of less-defined aggregation on CF agar." "Under these conditions, we found that the Δark mutant produced 8.2% of the wild-type level of viable spores. The data are consistent with the hypothesis that ark is required under abrupt or stringent starvation conditions and plays less of a role in slow nutrient depletion." Figure 4	challenge
acceptable	MYXXA:O52237	2013-05-23 19:49:25 CDT	GO:0042594 response to starvation (P)	PMID:16045620	ECO:0000315 mutant phenotype evidence used in manual assertion	Fig. 4. Developmental progression of wild-type cells and various mutants on TPM starvation agar during a 72 h time-course. Strains used were DK1622 (parent wild type), VP664 (brgE), DK7862 (sdeK), VP1014 (brgE sdeK), DK5208 (csgA) and VP825 (brgE csgA)	challenge
updatedbyinstructor	HUMAN:TGM2	2013-05-23 23:13:28 CDT	GO:0031226 intrinsic to plasma membrane (C)	PMID:2578891	ECO:0000314 direct assay evidence used in manual assertion	Figure 2, 3, 4: distribution of TGM activity	challenge
acceptable	ARATH:ARR12	2013-05-24 10:11:00 CDT	GO:0010082 regulation of root meristem growth (P)	PMID:23482873	ECO:0000315 mutant phenotype evidence used in manual assertion	Fig 1C "arr12 mutant exhibited enlarged meristem throughout this time period"	challenge
updatedbyinstructor	ARATH:ARR11	2013-05-24 10:12:42 CDT	GO:0010082 regulation of root meristem growth (P)	PMID:23482873	ECO:0000315 mutant phenotype evidence used in manual assertion	Fig 1C "weak effect on meristem size"	challenge
updatedbyinstructor	ARATH:ARP5	2013-05-24 10:14:07 CDT	GO:0010082 regulation of root meristem growth (P)	PMID:23482873	ECO:0000315 mutant phenotype evidence used in manual assertion	Fig 1C "weak effect on meristem"	challenge
updatedbyinstructor	ARATH:ARR10	2013-05-24 10:15:37 CDT	GO:0010082 regulation of root meristem growth (P)	PMID:23482873	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 1C: "mutant showed little effect after germination but more pronounced day 4 and thereafter"	challenge
updatedbyinstructor	ARATH:ARR1	2013-05-24 10:17:11 CDT	GO:0010082 regulation of root meristem growth (P)	PMID:23482873	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 1C "no strong effect til after day 4"	challenge
acceptable	ARATH:ARR12	2013-05-24 10:20:17 CDT	GO:0080036 regulation of cytokinin mediated signaling pathway (P)	PMID:23482873	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 2b	challenge
updatedbyinstructor	ARATH:ARR1	2013-05-24 10:21:30 CDT	GO:0080036 regulation of cytokinin-activated signaling pathway (P)	PMID:23482873	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 2b	challenge
updatedbyinstructor	ARATH:ARP5	2013-05-24 10:22:32 CDT	GO:0080036 regulation of cytokinin-activated signaling pathway (P)	PMID:23482873	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 2b	challenge
updatedbyinstructor	ARATH:ARR10	2013-05-24 10:23:37 CDT	GO:0080036 regulation of cytokinin-activated signaling pathway (P)	PMID:23482873	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 2B	challenge
requireschanges	MYXXA:O52237	2013-05-24 13:46:34 CDT	GO:0005515 protein binding (F)	PMID:16045620	ECO:0000021 physical interaction evidence	Fig 1 GST pulldown assay	challenge
updatedbyinstructor	ARATH:KRP5	2013-05-26 17:24:04 CDT	GO:0032877 positive regulation of DNA endoreduplication (P)	PMID:23426196	ECO:0000315 mutant phenotype evidence used in manual assertion	Fig2B	challenge
updatedbyinstructor	ARATH:KRP5	2013-05-26 17:36:14 CDT	GO:0031490 chromatin DNA binding (F)	PMID:23426196	ECO:0000314 direct assay evidence used in manual assertion	Figure 4	challenge
updatedbyinstructor	CYAM1:M1VBI3	2013-05-26 17:52:42 CDT	GO:0005739 mitochondrion (C)	PMID:23700350	ECO:0000314 direct assay evidence used in manual assertion	Figure 4G-J	challenge

acceptable:8
unacceptable:0
requires_changes:3
flagged:0

Annotations challenged by Marhuang

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
unacceptable	Michael B Chun, Team Tree	ARATH:RAR1	GO:0005515 - protein binding (F)	PMID:11847307	ECO:0000021 physical interaction evidence	challenge	C: 1
updatedbyinstructor	Ashayes, Team Mendel	ARATH:SUP	GO:0010093 - specification of floral organ identity (P)	PMID:1352237	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 3 fixed by Monica Yun
requireschanges	KShields, Team Mendel	ARATH:C3H49	GO:0009737 - response to abscisic acid (P)	PMID:22383628	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 1
updatedbyinstructor	KShields, Team Mendel	ARATH:LOX6	GO:0009611 - response to wounding (P)	PMID:23171345	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 1
updatedbyinstructor	Monica Yun, Team Beadle	ARATH:PLT5	GO:0010311 - lateral root formation (P)	PMID:23684976	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 1

0 annotations fixed by Marhuang

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