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User:Marhuang

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My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
updatedbyinstructorRAT:KCC2D2013-04-24 13:18:37 CDTGO:0004683 calmodulin-dependent protein kinase activity (F)PMID:22514276ECO:0000314 direct assay evidence used in manual assertion

Figure 1B. Immunoblot of HEK293 cells expressing Nav1.5 were exposed to rat CAMKIIdC showing phosphorylation of site on loop 1 of sodium channel.

challenge
acceptableRAT:KCC2D2013-04-26 10:43:03 CDTGO:0004672 protein kinase activity (F)PMID:22514276ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 5: Electrophysiology of channel with mutant phenotype T594A and S516A and also in comparison to drug AIP and WT phenotypes

challenge
requireschangesRAT:KCC2D2013-04-26 14:22:20 CDTGO:0005954 calcium- and calmodulin-dependent protein kinase complex (C)PMID:21677263ECO:0000314 direct assay evidence used in manual assertion

Figure 3: Immunoblot shows CaMKIId forms complex and increases activity of sodium channel

challenge
requireschangesRABIT:KCC2D2013-04-26 14:38:48 CDTGO:0004683 calmodulin-dependent protein kinase activity (F)PMID:21835909ECO:0000314 direct assay evidence used in manual assertion

Figure 6: Activation of CaMKII in rabbit cardiomyocytes using FRET

challenge
acceptableCHICK:EPHB22013-04-26 15:36:15 CDTGO:0021963 spinothalamic tract morphogenesis (P)PMID:19004859ECO:0000314 direct assay evidence used in manual assertion

Figure 4: Overexpression of EphB2 in neurogenic crest leads to ectopic DL migration

challenge
acceptableMYXXD:Q1DDD52013-04-27 14:38:36 CDTGO:0010468 regulation of gene expression (P)PMID:16484184ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 6: Wildtype vs mutant run on SDS-PAGE gel

challenge
acceptableHUMAN:TGM22013-05-23 13:24:40 CDTGO:0031226 intrinsic to plasma membrane (C)PMID:8105889ECO:0000315 mutant phenotype evidence used in manual assertion

TGK becomes quickly membrane bound. When mutated, TGK mutant is not membrane bound. Figure 2 identifies the localization of native and mutant using fractionation and then immunoblotting of the immunoprecipitation.

challenge
updatedbyinstructor?2013-05-23 15:00:01 CDTGO:0019867 outer membrane (Figure 2: Comparison to mutant xylella)PMID:20378647IMP: Inferred from Mutant Phenotype
challenge
acceptableMYXXD:Q1DA502013-05-23 19:41:45 CDTGO:0031155 regulation of fruiting body development (P)PMID:22389486ECO:0000315 mutant phenotype evidence used in manual assertion

"Surprisingly, development on CF agar was similar to that of the wild type, as the Δark mutant developed larger aggregates with slightly more defined edges than those for fruiting on TPM agar. The Δark mutant also developed a web-like appearance of less-defined aggregation on CF agar."

"Under these conditions, we found that the Δark mutant produced 8.2% of the wild-type level of viable spores. The data are consistent with the hypothesis that ark is required under abrupt or stringent starvation conditions and plays less of a role in slow nutrient depletion."

Figure 4

challenge
acceptableMYXXA:O522372013-05-23 19:49:25 CDTGO:0042594 response to starvation (P)PMID:16045620ECO:0000315 mutant phenotype evidence used in manual assertion

Fig. 4. Developmental progression of wild-type cells and various mutants on TPM starvation agar during a 72 h time-course. Strains used were

DK1622 (parent wild type), VP664 (brgE), DK7862 (sdeK), VP1014 (brgE sdeK), DK5208 (csgA) and VP825 (brgE csgA)

challenge
updatedbyinstructorHUMAN:TGM22013-05-23 23:13:28 CDTGO:0031226 intrinsic to plasma membrane (C)PMID:2578891ECO:0000314 direct assay evidence used in manual assertion

Figure 2, 3, 4: distribution of TGM activity

challenge
acceptableARATH:ARR122013-05-24 10:11:00 CDTGO:0010082 regulation of root meristem growth (P)PMID:23482873ECO:0000315 mutant phenotype evidence used in manual assertion

Fig 1C "arr12 mutant exhibited enlarged meristem throughout this time period"

challenge
updatedbyinstructorARATH:ARR112013-05-24 10:12:42 CDTGO:0010082 regulation of root meristem growth (P)PMID:23482873ECO:0000315 mutant phenotype evidence used in manual assertion

Fig 1C "weak effect on meristem size"

challenge
updatedbyinstructorARATH:ARP52013-05-24 10:14:07 CDTGO:0010082 regulation of root meristem growth (P)PMID:23482873ECO:0000315 mutant phenotype evidence used in manual assertion

Fig 1C "weak effect on meristem"

challenge
updatedbyinstructorARATH:ARR102013-05-24 10:15:37 CDTGO:0010082 regulation of root meristem growth (P)PMID:23482873ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1C: "mutant showed little effect after germination but more pronounced day 4 and thereafter"

challenge
updatedbyinstructorARATH:ARR12013-05-24 10:17:11 CDTGO:0010082 regulation of root meristem growth (P)PMID:23482873ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1C "no strong effect til after day 4"

challenge
acceptableARATH:ARR122013-05-24 10:20:17 CDTGO:0080036 regulation of cytokinin mediated signaling pathway (P)PMID:23482873ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2b

challenge
updatedbyinstructorARATH:ARR12013-05-24 10:21:30 CDTGO:0080036 regulation of cytokinin-activated signaling pathway (P)PMID:23482873ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2b

challenge
updatedbyinstructorARATH:ARP52013-05-24 10:22:32 CDTGO:0080036 regulation of cytokinin-activated signaling pathway (P)PMID:23482873ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2b

challenge
updatedbyinstructorARATH:ARR102013-05-24 10:23:37 CDTGO:0080036 regulation of cytokinin-activated signaling pathway (P)PMID:23482873ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2B

challenge
requireschangesMYXXA:O522372013-05-24 13:46:34 CDTGO:0005515 protein binding (F)PMID:16045620ECO:0000021 physical interaction evidence

Fig 1 GST pulldown assay

challenge
updatedbyinstructorARATH:KRP52013-05-26 17:24:04 CDTGO:0032877 positive regulation of DNA endoreduplication (P)PMID:23426196ECO:0000315 mutant phenotype evidence used in manual assertion

Fig2B

challenge
updatedbyinstructorARATH:KRP52013-05-26 17:36:14 CDTGO:0031490 chromatin DNA binding (F)PMID:23426196ECO:0000314 direct assay evidence used in manual assertion

Figure 4

challenge
updatedbyinstructorCYAM1:M1VBI32013-05-26 17:52:42 CDTGO:0005739 mitochondrion (C)PMID:23700350ECO:0000314 direct assay evidence used in manual assertion

Figure 4G-J

challenge

acceptable:8
unacceptable:0
requires_changes:3
flagged:0

Annotations challenged by Marhuang

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
unacceptableMichael B Chun,
Team Tree
ARATH:RAR1GO:0005515 - protein binding (F)PMID:11847307ECO:0000021 physical interaction evidencechallengeC: 1
updatedbyinstructorAshayes,
Team Mendel
ARATH:SUPGO:0010093 - specification of floral organ identity (P)PMID:1352237ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 3

fixed by Monica Yun
requireschangesKShields,
Team Mendel
ARATH:C3H49GO:0009737 - response to abscisic acid (P)PMID:22383628ECO:0000314 direct assay evidence used in manual assertionchallengeC: 1
updatedbyinstructorKShields,
Team Mendel
ARATH:LOX6GO:0009611 - response to wounding (P)PMID:23171345ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 1
updatedbyinstructorMonica Yun,
Team Beadle
ARATH:PLT5GO:0010311 - lateral root formation (P)PMID:23684976ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 1

0 annotations fixed by Marhuang

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