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User:Larichardson

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My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
updatedbyinstructorAZOVI:M9YCR72013-09-24 22:21:51 CDTGO:0045893 positive regulation of transcription, DNA-templated (P)PMID:21454367ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2 and qRT-PCR as described under "PsrA is required for maximal rpoS transcription and binds to the rpoS promoter region", data not presented in a figure. This reference uses strain ATCC9046, but the sequence was not available in UniProt so the accession for strain CA was used for the annotation.

challenge
unacceptableECOLI:CSPC2013-10-13 16:49:18 CDTGO:0003729 mRNA binding (F)PMID:23760898ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 3 shows that cspC mutant and cspC cspE double mutant have significantly lower transcript levels of rpoS than in the wild-type strain. The lack of rpoS transcripts in the mutants is assumed to be from the stabilization of the rpoS mRNA by CspC and CspE.

challenge

acceptable:0
unacceptable:1
requires_changes:0
flagged:0

Annotations challenged by Larichardson

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
requireschangesLleopold,
Team Protein Pushers
EBOZM:VP40GO:0020002 - host cell plasma membrane (C)PMID:23297401ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 1
requireschangesDrl5141,
Team Protein Titans
HUMAN:TRAIPGO:0034504 - protein localization to nucleus (P)PMID:19151749ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 1

0 annotations fixed by Larichardson

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