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User:Imumm

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My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptableHUMAN:DPP102012-02-12 16:27:24 CSTGO:0090004 positive regulation of establishment of protein localization in plasma membrane (P)PMID:15671030ECO:0000314 direct assay evidence used in manual assertion

Figure 1d showed that DPP10 facilitated cell membrane surface expression of Kv4.2 protein through in situ hybridization.

accession #:AY172661

challenge
acceptableRAT:DPP62012-02-07 19:10:30 CSTGO:0090004 positive regulation of establishment of protein localization in plasma membrane (P)PMID:15671030ECO:0000314 direct assay evidence used in manual assertion

figure 1c shows that DPPX (DPP6) promoted a robust trafficking of Kv4.2 to the plasma membrane.

accession #:M76427

challenge
acceptableHORVU:IAA12012-02-07 19:11:38 CSTGO:0015066 alpha-amylase inhibitor activity (F)PMID:2785932ECO:0000314 direct assay evidence used in manual assertion

Table 1

challenge
acceptableORYSJ:SLR12012-02-07 19:33:09 CSTGO:0005634 nucleus (C)PMID:10713441ECO:0000314 direct assay evidence used in manual assertion

Results section 3.5 and Figure 5 detail that nucleus localization occurs because of a bipartite NLS in the protein. Epifluorescence microscopy was used to determine the localization pattern.

challenge
acceptableMOUSE:LBH2012-02-12 16:25:19 CSTGO:0005634 nucleus (C)PMID:11336496ECO:0000314 direct assay evidence used in manual assertion

Section of the text labeled "Biochemical Characterization of Lbh protein" refers to N and C terminus labeling using an antibody to detect localization of the Lbh protein in the nucleus. (figure 2b)

challenge
acceptableHUMAN:CRCM2012-02-12 17:24:00 CSTGO:0005737 cytoplasm (C)PMID:18591935ECO:0000314 direct assay evidence used in manual assertion

Figure 1c showed Western Blot Analysis was used to confirm localization of the MCC protein to the cytoplasm.

challenge
acceptableHUMAN:CRCM2012-02-12 17:24:00 CSTGO:0005634 nucleus (C)PMID:18591935ECO:0000314 direct assay evidence used in manual assertion

Figure 1c showed the use of Western Blot Analysis to confirm MCC protein localization in the nucleus of cells.

challenge
requireschangesHUMAN:CRCM2012-02-12 17:24:00 CSTGO:0051726 regulation of cell cycle (P)PMID:18591935ECO:0000314 direct assay evidence used in manual assertion

Figure 4C and 4D shows that MCC regulates the cell cycle by suppressing the DNA-binding activity of β-catenin/TCF/LEF in the nucleus through Western Blot Analysis. This is also confirmed in the section of the text "MCC interacts with β-catenin, blocks β-catenin/TCF/LEF DNA binding, and can affect endogenous β-catenin localization/levels".

challenge
acceptableHUMAN:CRCM2012-02-12 17:24:00 CSTGO:0045184 establishment of protein localization (P)PMID:18591935ECO:0000314 direct assay evidence used in manual assertion

The section of the text "MCC interacts with β-catenin, blocks β-catenin/TCF/LEF DNA binding, and can affect endogenous β-catenin localization/levels" confirms that MCC relocalizes the β-catenin to the cytoplasm. The results are further confirmed through Western Blot Analysis in Figure 4.

challenge
requireschangesHUMAN:CRCM2012-02-12 15:50:37 CSTGO:0030308 negative regulation of cell growth (P)PMID:18591935ECO:0000314 direct assay evidence used in manual assertion

Immunofluorescence was used and confirmed in Figure 6A/B to show that MCC is responsible for the suppression of basal cell growth.

challenge
acceptableSHEEP:UTMP2012-02-12 16:22:57 CSTGO:0030414 peptidase inhibitor activity (F)PMID:8942989ECO:0000314 direct assay evidence used in manual assertion

Figure 2.a/b show evidence of %Pepsin activity severely decreased between pH of 2-4.5. Supported text for the experiment is listed under "Inhibitory Activities of Uterine Secretions" with standard assay.

challenge
acceptableHUMAN:DPP22012-03-27 20:13:07 CDTGO:0031982 vesicle (C)PMID:11067927ECO:0000314 direct assay evidence used in manual assertion

figure 5 shows through confocal microscopy, localization of QPP (DPP2) to the vesicles.

challenge
acceptableSTRPN:ZMPB2012-03-27 20:34:05 CDTGO:0009986 cell surface (C)PMID:10792723ECO:0000314 direct assay evidence used in manual assertion

figure 1b shows through immunoelectron microscopy, that ZmpB localizes to the cell surface.

challenge
requireschangesGLOHA:VMHA2012-03-27 20:45:45 CDTGO:0072577 endothelial cell apoptosis (P)PMID:14688240ECO:0000314 direct assay evidence used in manual assertion

Figure 7 shows endothelial cell apoptosis caused by halysase through fluorescence.

challenge
requireschangesARATH:AUX12012-04-10 19:59:23 CDTGO:0048767 root hair elongation (P)PMID:10036773ECO:0000314 direct assay evidence used in manual assertion

figures 4 and 5 show that AUX1 causes root hair development over time through microscopy.

challenge
requireschangesARATH:AUX12012-04-10 20:18:38 CDTGO:0009958 positive gravitropism (P)PMID:10205161ECO:0000269 experimental evidence used in manual assertion

figure 3 shows that AUX1 restores gravitropic root growth.

challenge
acceptableHUMAN:SYNE22012-04-14 11:40:19 CDTGO:0005634 nucleus (C)PMID:12118075ECO:0000314 direct assay evidence used in manual assertion

Figure 5 shows that NUANCE localizes to the nucleus through immunolabeling.

challenge
acceptableHUMAN:SYNE22012-04-14 11:42:49 CDTGO:0005737 cytoplasm (C)PMID:12118075ECO:0000314 direct assay evidence used in manual assertion

Figure 5 shows that NUANCE can localize to the cytoplasm through immunolabeling.

challenge

acceptable:13
unacceptable:0
requires_changes:5
flagged:0

Annotations challenged by Imumm

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
requireschangesHilaryann0524,
Team Unicorn
CAEEL:MES4GO:0016571 - histone methylation (P)PMID:20824077ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 1
requireschangesHilaryann0524,
Team Unicorn
HUMAN:BMI1GO:0043526 - obsolete neuroprotection (P)PMID:22384090ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 1
requireschangesBhatt001,
Team The Histones
HUMAN:SERPHGO:0005783 - endoplasmic reticulum (C)PMID:20188343ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 2
requireschangesSt413403,
Team James Watson
ECOLI:FTSZGO:0003924 - GTPase activity (F)PMID:22056926ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 2

0 annotations fixed by Imumm