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User:Hughe017

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My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptableMOUSE:GDF82012-02-12 17:15:20 CSTGO:0048632 negative regulation of skeletal muscle tissue growth (P)PMID:21390326ECO:0000315 mutant phenotype evidence used in manual assertion

Table 1 and Figure 2 show the Gastrocnemius, Quadriceps, and Triceps muscles all were significantly larger in weight(g) in myostatin deficient mice compared to normal myostatin mice.

challenge
unacceptableMOUSE:GDF82012-02-12 15:56:50 CSTGO:0019222 regulation of metabolic process (P)PMID:11877467ECO:0000315 mutant phenotype evidence used in manual assertion

Table 2 shows Mstn–/– mice had higher rates of total and resting O2 consumption compared with Mstn+/+ mice, which is expected given the higher body weights of Mstn–/– mice. If the data are expressed as a function of body weight, however, Mstn–/– mice actually had lower rates of total and resting O2 consumption compared with Mstn+/+ mice.

challenge
unacceptableMOUSE:GDF82012-02-12 17:00:32 CSTGO:0019217 regulation of fatty acid metabolic process (P)PMID:21390326ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 4 shows mg fatty acids per g of muscle tissue is significantly less in myostatin depleted mice fed a high-fat diet.

challenge
unacceptableMOUSE:GDF82012-02-12 17:00:32 CSTGO:0043610 regulation of carbohydrate utilization (P)PMID:21390326ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 3 shows blood glucose concentration is significantly lower in myostatin depleted mice fed a high-fat diet for 5 months, 90 min after ip glucose injection.

challenge
unacceptableMOUSE:GDF82012-02-12 16:58:42 CSTPMID:21390326ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 5 shows that myostatin depleted mice have a significantly lower incidence and severity of hepatic statosis when fed a high-fat diet for 5 months.

challenge
acceptableMOUSE:GDF82012-02-12 17:34:41 CSTGO:0048632 negative regulation of skeletal muscle tissue growth (P)PMID:11877467ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1a shows the increased mass of triceps muscle in Mstn -/- mice at different ages as well as an apparent dose-dependency on muscle mass.

challenge
requireschangesMOUSE:GDF82012-02-12 17:54:06 CSTGO:0019216 regulation of lipid metabolic process (P)PMID:11877467ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1b-g shows the significant differences in fat pad mass between Mstn -/- and Mstn +/+ mice after 2 months of age.

challenge
acceptableMOUSE:M3K52012-02-22 12:43:41 CSTGO:0045663 positive regulation of myoblast differentiation (P)PMID:22337877ECO:0000314 direct assay evidence used in manual assertion

Fig. 3

challenge
acceptableMOUSE:NR2C22012-02-22 12:47:10 CSTGO:0045663 positive regulation of myoblast differentiation (P)PMID:22337877ECO:0000314 direct assay evidence used in manual assertion

Fig. 2

challenge
acceptableMOUSE:M3K52012-02-22 18:10:33 CSTGO:0038066 p38MAPK cascade (P)PMID:22337877ECO:0000270 expression pattern evidence used in manual assertion

Fig. 4

challenge
acceptableMOUSE:NR2C22012-02-22 18:12:31 CSTGO:0038066 p38MAPK cascade (P)PMID:22337877ECO:0000270 expression pattern evidence used in manual assertion

Fig. 4

challenge
acceptableMOUSE:DLX32012-02-23 23:39:33 CSTGO:0071895 odontoblast differentiation (P)PMID:22351765ECO:0000315 mutant phenotype evidence used in manual assertion

Fig. 4 and Fig. 5

challenge
requireschangesECOBW:RRMF2012-02-26 17:28:53 CSTGO:2000234 positive regulation of rRNA processing (P)PMID:20807199ECO:0000270 expression pattern evidence used in manual assertion

Fig. 3

challenge
acceptableMOUSE:CADH12012-03-09 18:32:54 CSTGO:0035847 uterine epithelium development (P)PMID:22378759ECO:0000315 mutant phenotype evidence used in manual assertion

Fig. 1

challenge
acceptableMOUSE:CADH12012-03-09 18:32:54 CSTGO:0007566 embryo implantation (P)PMID:22378759ECO:0000315 mutant phenotype evidence used in manual assertion

Fig. 5

challenge
acceptableMOUSE:CADH12012-03-09 18:32:54 CSTGO:0046697 decidualization (P)PMID:22378759ECO:0000315 mutant phenotype evidence used in manual assertion

Fig. 5c

challenge
acceptableARATH:PUB132012-03-09 20:46:40 CSTGO:0043066 negative regulation of apoptotic process (P)PMID:22383540ECO:0000315 mutant phenotype evidence used in manual assertion

Fig. 3

challenge
acceptableARATH:PUB132012-03-09 20:46:41 CSTGO:2000028 regulation of photoperiodism, flowering (P)PMID:22383540ECO:0000315 mutant phenotype evidence used in manual assertion

Fig. 7. Results were most notable in MD exposure. Negative regulator of flowering.

challenge
acceptableDROME:Q0KHV62012-03-18 14:42:09 CDTGO:0047497 mitochondrion transport along microtubule (P)PMID:22396657ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 3

challenge
acceptableDROME:Q0KHV62012-03-18 14:42:09 CDTGO:0048312 intracellular distribution of mitochondria (P)PMID:22396657ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 4

challenge
acceptableMOUSE:MNS12012-03-18 15:00:42 CDTGO:0007283 spermatogenesis (P)PMID:22396656ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2

challenge
acceptableMOUSE:MNS12012-03-18 15:00:42 CDTGO:0045724 positive regulation of flagellum assembly (P)PMID:22396656ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 3

challenge
acceptableMOUSE:ABHD52012-03-18 15:54:56 CDTGO:0050996 positive regulation of lipid catabolic process (P)PMID:22383684ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 4

challenge
requireschangesHUMAN:UTP62012-04-01 17:15:29 CDTGO:0015935 small ribosomal subunit (C)PMID:22434888ECO:0000270 expression pattern evidence used in manual assertion

Fig. 1,2,3

challenge
requireschangesHUMAN:UTP62012-04-01 17:15:29 CDTGO:0046601 positive regulation of centriole replication (P)PMID:22434888ECO:0000270 expression pattern evidence used in manual assertion

Fig. 7

challenge
requireschangesHELPX:P948322012-04-15 11:28:21 CDTGO:0052058 modification by symbiont of host morphology or physiology via substance secreted by type IV secretion system (P)PMID:22493745ECO:0000315 mutant phenotype evidence used in manual assertion

Fig. 3

challenge
requireschangesMOUSE:Q8CGC32012-04-15 12:29:38 CDTGO:0090044 positive regulation of tubulin deacetylation (P)PMID:22492994ECO:0000270 expression pattern evidence used in manual assertion

Fig. 6

challenge
requireschangesMOUSE:SWI52012-04-15 12:50:21 CDTGO:0000730 DNA recombinase assembly (P)PMID:22492707ECO:0000270 expression pattern evidence used in manual assertion

Fig. 2 shows that the SWI5-SFR1 complex functions in stabilization of the Rad51 filament

challenge
requireschangesMOUSE:SFR12012-04-15 12:53:09 CDTGO:0000730 DNA recombinase assembly (P)PMID:22492707ECO:0000270 expression pattern evidence used in manual assertion

Fig. 2 shows the SWI5-SFR1 complex functions in stabilizing the Rad51 filament

challenge

acceptable:17
unacceptable:4
requires_changes:8
flagged:0

Annotations challenged by Hughe017

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
requireschangesEwillis,
Team Bazinga
RAT:RNF4GO:0005634 - nucleus (C)PMID:9710597ECO:0000314 direct assay evidence used in manual assertionchallengeC: 1
unacceptableJchurch,
Team Bazinga
HUMAN:NS1BPGO:0044419 - biological process involved in interspecies interaction between organisms (P)PMID:9696811ECO:0000314 direct assay evidence used in manual assertionchallengeC: 1
requireschangesNelso186,
Team Dinosaurs are Awesome
ARATH:AFP1GO:0048519 - negative regulation of biological process (P)PMID:12569131ECO:0000314 direct assay evidence used in manual assertionchallengeC: 2
requireschangesHarpe013,
Team Dinosaurs are Awesome
HUMAN:JAK2GO:0042325 - regulation of phosphorylation (P)PMID:22319590ECO:0000314 direct assay evidence used in manual assertionchallengeC: 3
requireschangesHarpe013,
Team Dinosaurs are Awesome
MOUSE:ZN521GO:0045814 - negative regulation of gene expression, epigenetic (P)PMID:19049973ECO:0000270 expression pattern evidence used in manual assertionchallengeC: 1
requireschangesSt413403,
Team James Watson
ECOLI:FTSZGO:0051301 - cell division (P)PMID:2045370ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 2

0 annotations fixed by Hughe017