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User:Harsharn.kaur

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Spring 2013

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
updatedbyinstructorHUMAN:P532013-04-11 11:23:13 CDTGO:0008285 negative regulation of cell proliferation (P)PMID:22783376ECO:0000314 direct assay evidence used in manual assertion

Figure 3A: Cell proliferation assay data was performed at 48 h to examine the effect of cytoplasmically localized wild-type p53 and the S121F mutant on cell proliferation. The data resulted with both wild-type p53 and the S121F mutant inhibiting cell proliferation.

challenge
unacceptableHUMAN:P532013-04-12 15:13:28 CDTGO:0003677 DNA binding (F)PMID:23555710ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 3B shows that without the critical role of CTDBS hot spot mutp53 proteins R175H, R273H and R248W are unable to selectively recognize scDNA.

challenge
unacceptableHUMAN:P532013-04-13 16:28:03 CDTGO:0003677 DNA binding (F)PMID:23555710ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 7 shows that DNA topology influences the mutp53-driven repression on the system of BAX and MSP/MST1 promoters, regulated by mutp53s in H1299 and Saos2 cells.

challenge
acceptableYEAST:LSB12013-04-13 19:26:51 CDTGO:0034316 negative regulation of Arp2/3 complex-mediated actin nucleation (P)PMID:23577202ECO:0000314 direct assay evidence used in manual assertion

figure 2 show that purified Lsb1 and Lsb2 full-length proteins had an inhibitory activity on Las17-dependent actin polymerization in vitro. These results were further confirmed by measuring the KD constants of their interaction with Las17.

challenge

acceptable:1
unacceptable:2
requires_changes:0
flagged:0

Annotations challenged by Harsharn.kaur

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
acceptableDjgulizia,
Team Texas Aggies
HUMAN:FLNAGO:2001046 - positive regulation of integrin-mediated signaling pathway (P)PMID:18177638ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 1
acceptableStanto81,
Team Bringin back Fanny Packs
SCHPO:DCR1GO:0010629 - negative regulation of gene expression (P)PMID:17981703ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 1
acceptableDjgulizia,
Team Texas Aggies
MOUSE:WNT3GO:0030177 - positive regulation of Wnt signaling pathway (P)PMID:23324743ECO:0000314 direct assay evidence used in manual assertionchallengeC: 1
updatedbyinstructorAndrew.james,
Team Gene Wilder
DROME:Q9Y1L3GO:0140658 - ATP-dependent chromatin remodeler activity (F)PMID:18250149ECO:0000314 direct assay evidence used in manual assertionchallengeC: 4
correctedthroughchallengesDylandekalb,
Team Higgs BROsons
MOUSE:LIS1GO:0002934 - desmosome organization (P)PMID:21844209ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 1

fixed by Harsharn.kaur
updatedbyinstructorFkmasuda,
Team The Z-team
DANRE:Q9DD89GO:0004190 - aspartic-type endopeptidase activity (F)PMID:21747967ECO:0000314 direct assay evidence used in manual assertionchallengeC: 3
updatedbyinstructorDimitrios.Spantithos,
Team Gene Shalit
ECOLI:YBJNGO:1900232 - negative regulation of single-species biofilm formation on inanimate substrate (P)PMID:21980417ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 1

fixed by Harsharn.kaur
updatedbyinstructorSinghpr4,
Team The Monstars
BRASZ:NAABGO:0051213 - dioxygenase activity (F)PMID:21498645ECO:0000314 direct assay evidence used in manual assertionchallengeC: 2

fixed by Harsharn.kaur
updatedbyinstructorReddenc1,
Team Better Than Hot Pocket
XANC5:Q3BUB5GO:0043709 - cell adhesion involved in single-species biofilm formation (P)PMID:21995764ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 6

3 annotations fixed by Harsharn.kaur

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Fall 2012

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
unacceptableRAT:NRP12012-10-29 20:02:45 CDTGO:0090211 positive regulation of establishment of blood-brain barrier (P)PMID:11018070ECO:0000314 direct assay evidence used in manual assertion

Figure 1g: Quantitative Data was analyzed that showed a significant amount of increase for rhVEGF165 treated group in plasma perfusion in vessels within 3μm of diameters compared to the data for saline-treated control group.

challenge
unacceptableRAT:NRP12012-10-29 21:21:10 CDTGO:0090212 negative regulation of establishment of blood-brain barrier (P)PMID:ECO:0000314 direct assay evidence used in manual assertion

Figure 4: A coronal section was examined with areas of gross hemorrhage from the rhVEGF165 treated rats at 24 hours after a stroke which corresponded to the MRI section (large hyperintense areas on Gd-DTPA observed after 30 minutes from injecting Gd-DTPA into rhVEGF165 rats), "Suggests that the early increase in BBB leakage to large molecules may be related to the incidence of hemorrhage in this model".

challenge
unacceptableRAT:NRP12012-10-29 21:45:10 CDTGO:0008015 blood circulation (P)PMID:11018070ECO:0000314 direct assay evidence used in manual assertion

Figure 7: A transient increase of Cerebal blood flow in the ischemic brain was observed via early administration of rhVEGF165 in rats.

challenge
updatedbyinstructorLAMBD:GAM2012-11-18 15:53:32 CSTGO:0060703 deoxyribonuclease inhibitor activity (F)PMID:4275917ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2 provides the evidence that "product of the gam gene of phage lambda (gamma protein) is probably itself an inhibitor of the RecBC DNase".

challenge
updatedbyinstructorLAMBD:GAM2012-11-18 16:52:33 CSTGO:0060703 deoxyribonuclease inhibitor activity (F)PMID:4275917ECO:0000314 direct assay evidence used in manual assertion

Figure 3: "The gamma protein was purified on the basis of its capacity

to inhibit the ATP-dependent, double-strand exonuclease activity of the RecBC DNase.In the presence of inhibitor, the nuclease reaction proceeded at a constant, but reduced rate throughout the time of incubation due to the presence of inactive gamma protein that can compete with active material".

challenge

acceptable:0
unacceptable:3
requires_changes:0
flagged:0

Annotations challenged by Harsharn.kaur

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
requireschangesMtaya1,
Team Rocket
BORBR:BVGSGO:0009405 - obsolete pathogenesis (P)PMID:9194696ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 2
requireschangesKpadda1,
Team Pterodactyl
HELPY:FLAAGO:0071978 - bacterial-type flagellum-dependent swarming motility (P)PMID:8501031ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 1

0 annotations fixed by Harsharn.kaur