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User:Fpadua

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CACAO Spring 2014

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
unacceptableHUMAN:CHK22014-02-02 14:55:55 CSTGO:0042127 regulation of cell proliferation (P)PMID:14608368ECO:0000314 direct assay evidence used in manual assertion

Figure 1.n. shows that the expression levels of this protein do not differ between senescent and proliferating cells.

challenge
unacceptableHUMAN:CHK22014-02-02 14:55:55 CSTGO:0042127 regulation of cell proliferation (P)PMID:14608368ECO:0000314 direct assay evidence used in manual assertion

Figure 1.n. shows that phosphorylation at threonine 68 is upregulated in senescent cells compared to proliferating cells

challenge
unacceptableHUMAN:CHK12014-02-02 14:59:44 CSTPMID:14608368ECO:0000314 direct assay evidence used in manual assertion

Figure 1.m. shows that phosphorylation at serine 345 is upregulated in senescent cells compared to proliferating cells.

challenge
unacceptableHUMAN:SMC1A2014-02-02 15:03:45 CSTGO:0042127 regulation of cell proliferation (P)PMID:14608368ECO:0000314 direct assay evidence used in manual assertion

Fig 1 (k) shows that phosphorylation of serine 966 is upregulated in senescent cells compared to proliferating cells.

challenge
unacceptableHUMAN:RAD172014-02-02 15:22:19 CSTGO:0042127 regulation of cell proliferation (P)PMID:14608368ECO:0000314 direct assay evidence used in manual assertion

Figure 1 L shows that phosphorylation at serine 645 is conserved among senescent cells, late population doubling cells, and undamaged proliferating cells.

challenge
updatedbyinstructorHUMAN:SIR12014-02-16 14:31:34 CSTGO:0071441 negative regulation of histone H3-K14 acetylation (P)PMID:17916362ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1A: Inhibition of this protein with sirtinol increased acetylation of histone H3 (Lys14).

challenge
updatedbyinstructorHUMAN:SIR12014-02-16 14:31:34 CSTGO:2000619 negative regulation of histone H4-K16 acetylation (P)PMID:17916362ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1A: Inhibition with sirtinol increased the acetylation of histone H4 (Lys16)

challenge
unacceptableHUMAN:SIR12014-02-16 14:31:35 CSTGO:0044154 histone H3-K14 acetylation (P)PMID:17916362ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1A & 1D: Knockdown with Sirt1 siRNA led to increased acetylation of H3 (Lys14).

challenge
unacceptableHUMAN:SIR12014-02-16 14:31:36 CSTGO:0043983 histone H4-K12 acetylation (P)PMID:17916362ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1A and 1D: Knockdown with Sirt1 siRNA increased acetylation of H4 (Lys 16).

challenge
unacceptableHUMAN:SIR12014-02-16 15:54:35 CSTGO:0042127 regulation of cell proliferation (P)PMID:17916362ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1B: Inhibition by sirtinol showed decreased cell proliferation in a dose-dependent manner.

challenge
unacceptableHUMAN:SIR12014-02-16 15:54:35 CSTGO:0042127 regulation of cell proliferation (P)PMID:17916362ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1B: Knockdown with Sirt1 siRNA decreased cellular proliferation, but not to the same extent of that by inhibition with sirtinol.

challenge
unacceptableHUMAN:SIR12014-02-16 15:54:36 CSTGO:0006260 DNA replication (P)PMID:17916362ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1C: Inhibition of Sirt1 with sirtinol decreased the DNA replication as noted by BrdU incorporation.

challenge
unacceptableHUMAN:SIR12014-02-16 15:54:37 CSTGO:0006260 DNA replication (P)PMID:17916362ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1C: Knockdown of Sirt1 with Sirt1 siRNA decreased DNA replication as measured by BrdU incorporation to a lesser extent than by inhibition with sirtinol.

challenge
updatedbyinstructorHUMAN:SIR12014-02-16 15:54:38 CSTGO:0090400 stress-induced premature senescence (P)PMID:17916362ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1B: Inhibition by sirtinol showed decreased cell proliferation in a dose-dependent manner. Figure 2B: Inhibition with sirtinol increased senescence-associated beta-galactosidase activity.

challenge
unacceptableHUMAN:SIR12014-02-16 15:54:39 CSTGO:0090400 stress-induced premature senescence (P)PMID:17916362ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2B: Knockdown with Sirt1 siRNA increased senescence-associated beta-galactosidase activity.

challenge
unacceptableHUMAN:SIR12014-02-16 15:54:39 CSTGO:0090400 stress-induced premature senescence (P)PMID:17916362ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2A: Inhibition with sirtinol caused cells to exhibit senescent-like morophological changes (enlarged and flattened shapes).

challenge
unacceptableHUMAN:SIR12014-02-16 15:54:40 CSTGO:0090400 stress-induced premature senescence (P)PMID:17916362ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2A: Knockdown with Sirt1 siRNA caused cells to exhibit senescent-like morophological changes (enlarged and flattened shapes).

challenge
updatedbyinstructorHUMAN:SIR12014-02-16 15:54:41 CSTGO:1901984 negative regulation of protein acetylation (P)PMID:17916362ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 3A, 3C: Inhibition by sirtinol increased the acetylation of p53 after 3 days.

challenge
unacceptableHUMAN:SIR12014-02-16 15:54:41 CSTGO:0006473 protein acetylation (P)PMID:17916362ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 3A, 3C & 3D: Knockdown with Sirt1 siRNA increased the acetylation of p53 after 3 days.

challenge
updatedbyinstructorHUMAN:SIR12014-02-16 15:54:42 CSTGO:0010629 negative regulation of gene expression (P)PMID:17916362ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 3A, 3C: Inhibition by sirtinol increased the expression of p53 after 5 days.

challenge
unacceptableHUMAN:SIR12014-02-16 15:54:43 CSTGO:0010468 regulation of gene expression (P)PMID:17916362ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 3A, 3C & 3D: Knockdown with Sirt1 siRNA increased the expression of p53 after 5 days.

challenge

acceptable:0
unacceptable:16
requires_changes:0
flagged:0

Annotations challenged by Fpadua

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0 annotations fixed by Fpadua

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