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User:FFitzgerald

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CACAO Spring 2019

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptableCAUVC:Q9AAR62019-01-29 16:03:36 CSTGO:0000160 phosphorelay signal transduction system (P)PMID:16176121ECO:0001202 in vitro protein kinase assay evidence used in manual assertion

Fig 5 showed phosphorylation of the kinase CC0530 (CenK) to reveal the regulator CC3743 (CenR)

Organism:Caulobacter Crescentus

challenge
acceptable9CAUD:H6X3K72019-02-12 16:03:40 CSTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Through Mass Spectroscopy, Table S3 states that gp050 (Gene Rak2_00050) proposed function in Enterobacteria Phage vB_KleM-Rak2 is identified as a Conserved Phage protein with a Molecular Weight of 33.576.

challenge
acceptable9CAUD:A0A220GHA52019-02-12 16:12:27 CSTGO:0098672 evasion by virus of CRISPR-cas system (P)PMID:28785032ECO:0007040 plaque assay evidence used in manual assertion

Figure 2b shows how the activity of an Anti-CRISPR protein (acrIIA5) with A CR1 immune cell in a Streptococcus phage (D4276) can increase sensitivity to cos-type phage D5842

challenge
acceptable9CAUD:H6X4Z12019-03-19 16:15:35 CDTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Fig 5 shows that gp534(ORF534) produces a protein residue via SDS-PAGE. Table S3 states that gp534 is a putative virion protein with a molecular weight of 70.781 due to bioinformatics.

Organism: Klebsiella Phage vB_KleM-Rak2

challenge
acceptable9CAUD:H6X4Y32019-03-19 15:03:41 CDTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Through MS/MS, Figure 5 shows that gp526w(ORF526w), a putative tail protein, is a protein residue via SDS-PAGE. Through bioinformatics, shown in Table S3, the protein was determined to be a putative tail protein and has a molecular weight of 63.021.

Organism: Klebsiella PhagevB_KleM-Rak2.

challenge
acceptable9CAUD:H6X4Y42019-03-19 15:08:37 CDTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Through MS/MS, shown in figure 5, it is determined that gp527w(ORF527w) is present in mature virus particles via SDS-PAGE. Through bioinformatics, shown in Table S3, the protein was determined to be a putative tail fiber protein with a molecular weight of 79.449 in Klebsiella Phage vB_KleM-RaK2.

challenge
acceptable9CAUD:H6X4Y72019-03-19 15:13:54 CDTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Figure 5 identifies the protein gp530w through Mass spectrometry and it is defined as a putative tail protein through bioinformatics in table S3. Gp530w is a structural protein of Klebsiella Phage vB_KleM-RaK.

challenge
acceptable9CAUD:H6X4782019-03-19 15:20:32 CDTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Through Mass spectrometry, Figure 5 identifies the protein gp 531w(ORF531w), a structural protein, via SDS-PAGE. It is defined as a putative tail protein through bioinformatics in table S3 with a molecular weight of 97.727. Gp531w is a structural protein of Klebsiella Phage vB_KleM-RaK.

challenge
acceptable9CAUD:H6X3R22019-03-25 20:57:12 CDTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Fig 5 shows that gp105(ORF105), a putative tail protein of Klebsiella Phage vB_KleM-RaK2, is a protein residue via SDS-PAGE. Table S3 states that the proposed function is a putative virion protein with a molecular weight of 27.854.

challenge
acceptable9CAUD:H6X3R32019-03-25 20:55:10 CDTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Fig 5 shows that gp106(ORF106w), a putative tail protein of Klebsiella Phage vB_KleM-RaK2, is a protein residue via SDS-PAGE. Table S3 states that the proposed function is a putative virion protein with a molecular weight of 24.651 through bioinformatics.

challenge
acceptable9CAUD:H6X3R42019-03-25 20:46:56 CDTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Fig 5 shows that gp107(ORF107), a putative virion protein of Klebsiella Phage vB_KleM-RaK2, is a protein residue via SDS-PAGE. Table S3 states that the proposed function is a putative virion protein with a molecular weight of 21.133.

challenge
acceptable9CAUD:H6X3U22019-03-25 20:53:22 CDTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Fig 5 shows that gp135(ORF135), a putative virion protein of Klebsiella Phage vB_KleM-RaK2, is a protein residue via SDS-PAGE. Table S3 states that the proposed function is a putative virion protein with a molecular weight of 23.259.

challenge
updatedbyinstructor9CAUD:A0A2P1CKQ22019-03-26 16:21:33 CDTGO:0019012 virion (C)PMID:30366363ECO:0001582 liquid chromatography coupled with tandem mass spectrometry evidence used in manual assertion

The tail fiber protein (gp25, ORF25) is identified through LC-MS/MS in Pantoea phage vB_PagS_Vid5. Supplementary Table 3 shows the data obtained from the LC-MS/MS and identifies the molecular weight as 44.464(KDa).

challenge
updatedbyinstructor9CAUD:A0A2P1CKH42019-04-09 15:43:55 CDTGO:0019012 virion (C)PMID:30366363ECO:0001582 liquid chromatography coupled with tandem mass spectrometry evidence used in manual assertion

The major capsid protein (gp06, ORF06) is identified through LC-MS/MS in Pantoea phage vB_PagS_Vid5. Supplementary Table 3 shows the data obtained from the LC-MS/MS and identifies the molecular weight as 39.892 (KDa)

challenge
updatedbyinstructor9CAUD:A0A2P1CKN72019-04-09 15:47:11 CDTGO:0019012 virion (C)PMID:30366363ECO:0001582 liquid chromatography coupled with tandem mass spectrometry evidence used in manual assertion

The major tail protein (gp15, ORF15) is identified through LC-MS/MS in Pantoea phage vB_PagS_Vid5. Supplementary Table 3 shows the data obtained from the LC-MS/MS and identifies the molecular weight as 33.329(KDa).

challenge
acceptable9CAUD:H6X3L72019-04-09 16:28:57 CDTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Fig 5 shows that gp060w(ORF060w), a putative virion protein of Klebsiella Phage vB_KleM-RaK2, is a protein residue via SDS-PAGE. Table S3 states that the proposed function is a putative virion protein with a molecular weight of 56.225(KDa).

Uniprot: Uncharacterized protein, RaK2_00060

challenge
acceptable9CAUD:H6X3L82019-04-09 16:27:31 CDTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Fig 5 shows that gp061w(ORF61w), a putative virion protein of Klebsiella Phage vB_KleM-RaK2, is a protein residue via SDS-PAGE. Table S3 states that the proposed function is a putative virion protein with a molecular weight of 12.577(KDa).

Uniprot: Uncharacterized protein, RaK2_00061

challenge
acceptable9CAUD:H6X3M22019-04-14 22:01:17 CDTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Fig 5 shows that gp065(ORF065), a putative virion protein of Klebsiella Phage vB_KleM-RaK2, is a protein residue via SDS-PAGE. Table S3 states that the proposed function is a putative virion protein with a molecular weight of 45.317 (KDa).

Uniprot: Uncharacterized protein, RaK2_00065

challenge
acceptable9CAUD:H6X3M52019-04-14 22:07:00 CDTGO:0019012 virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertion

Fig 5 shows that gp068w(ORF068), a putative virion protein of Klebsiella Phage vB_KleM-RaK2, is a protein residue via SDS-PAGE. Table S3 states that the proposed function is a putative virion protein with a molecular weight of 56.144 (KDa).

Uniprot: Uncharacterized protein, RaK2_00068

challenge

acceptable:16
unacceptable:0
requires_changes:0
flagged:0

Annotations challenged by FFitzgerald

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
updatedbyinstructorMAntonescu,
Death by Gene
LAMBD:SPAN1GO:0061025 - membrane fusion (P)PMID:25870259ECO:0007122 in vitro assay evidence used in manual assertionchallengeC: 2

fixed by FFitzgerald

1 annotations fixed by FFitzgerald