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User:Dimitrios.Spantithos

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Spring 2013

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
correctedthroughchallengesECOK1:A1A9922013-03-28 13:05:13 CDTGO:0051271 negative regulation of cellular component movement (P)PMID:21980417ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 4B shows YbjN suppresses bacterial motility.

challenge
correctedthroughchallengesECOLW:E0IZI82013-03-28 13:19:20 CDTGO:0030092 regulation of flagellum assembly (P)PMID:21980417ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 4C shows over-expression of ybjN negatively regulates flagellar gene expression.

challenge
correctedthroughchallengesECOK1:A1A9922013-03-28 14:00:40 CDTGO:0009242 colanic acid biosynthetic process (P)PMID:21980417ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 5 shows negatively regulates colanic acid biosynthesis.

challenge
updatedbyinstructorECOLI:YBJN2013-03-30 13:26:59 CDTGO:1902209 negative regulation of bacterial-type flagellum assembly (P)PMID:21980417ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 4C over-expression of ybjN negatively regulates flagellar gene expression.

challenge
updatedbyinstructorECOLI:YBJN2013-03-30 13:27:00 CDTGO:1900232 negative regulation of single-species biofilm formation on inanimate substrate (P)PMID:21980417ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 6 shows YbjN is a negative regulator of biofilm formation.

challenge
updatedbyinstructorECOLI:YBJN2013-03-30 13:27:00 CDTGO:0009297 pilus assembly (P)PMID:21980417ECO:0000315 mutant phenotype evidence used in manual assertion

As shown in figure 3C, fimbriae are either malformed or not formed at all in cells with ybjN expression, whereas wild type cells produced substantial fimbriae.

challenge
acceptableECOLI:YBJN2013-03-30 13:27:00 CDTGO:0030308 negative regulation of cell growth (P)PMID:21980417ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2 shows over-expression of ybjN leads to growth inhibition

challenge

acceptable:1
unacceptable:0
requires_changes:0
flagged:0

Annotations challenged by Dimitrios.Spantithos

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
updatedbyinstructorNfarmer91,
Team Bonfire
MOUSE:NF2L2GO:1902037 - negative regulation of hematopoietic stem cell differentiation (P)PMID:23434824ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 2

fixed by Nicole.enochs

0 annotations fixed by Dimitrios.Spantithos

What do the icons mean in the status column?

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Fall 2012

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
requireschangesSTRDO:GTF22012-10-03 20:43:04 CDTGO:0046527 glucosyltransferase activity (F)PMID:22361861ECO:0000314 direct assay evidence used in manual assertion

Figure 2, Figure 5

challenge
acceptableHUMAN:RYR12012-11-12 17:27:21 CSTGO:0005219 ryanodine-sensitive calcium-release channel activity (F)PMID:22480578ECO:0000314 direct assay evidence used in manual assertion

Figure 4A and Figure 4B

challenge
unacceptableMOUSE:CASK2012-11-12 19:06:20 CSTGO:0004860 protein kinase inhibitor activity (F)PMID:21173178ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 4.

challenge
unacceptableHUMAN:CATS2012-11-12 22:50:32 CSTGO:0030162 regulation of proteolysis (P)PMID:22864553ECO:0000314 direct assay evidence used in manual assertion

Figure 6.

challenge
unacceptableMOUSE:RYR12012-11-13 00:33:44 CSTGO:0005219 ryanodine-sensitive calcium-release channel activity (F)PMID:23131566ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1C, Figure 3C

challenge
requireschangesMOUSE:Q1JUQ62012-11-13 00:46:56 CSTGO:0005528 FK506 binding (F)PMID:23131566ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 4A, C, and D.

challenge
unacceptableMOUSE:RYR12012-11-13 00:54:33 CSTGO:0060315 negative regulation of ryanodine-sensitive calcium-release channel activity (P)PMID:23131566ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 4A.

challenge
unacceptableMOUSE:RYR12012-11-13 00:56:29 CSTGO:0060314 regulation of ryanodine-sensitive calcium-release channel activity (P)PMID:23131566ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 4A.

challenge
requireschangesMOUSE:RYR12012-11-13 00:58:24 CSTGO:0005219 ryanodine-sensitive calcium-release channel activity (F)PMID:23131566ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 4A.

challenge
unacceptableLACRD:A5VL732012-11-13 14:20:28 CSTGO:0046527 glucosyltransferase activity (F)PMID:22361861ECO:0000314 direct assay evidence used in manual assertion

Figure 2, Figure 5

challenge
acceptableLACRD:A5VL732012-11-13 14:31:36 CSTGO:0032001 1,4-alpha-glucan 6-alpha-glucosyltransferase activity (F)PMID:22361861ECO:0000314 direct assay evidence used in manual assertion

Figure 2, Figure 5

challenge
acceptableLACRD:A5VL732012-11-13 14:33:33 CSTGO:0033825 oligosaccharide 4-alpha-D-glucosyltransferase activity (F)PMID:22361861ECO:0000314 direct assay evidence used in manual assertion

Figure 2, Figure 5

challenge
unacceptableMOUSE:GABR12012-11-13 15:41:23 CSTGO:0007214 gamma-aminobutyric acid signaling pathway (P)PMID:22504960ECO:0000314 direct assay evidence used in manual assertion

Figure 6B

Intracellular cAMP level was completely suppressed by baclofen.

challenge
unacceptableMOUSE:GABR12012-11-13 15:45:18 CSTGO:0004965 G-protein coupled GABA receptor activity (F)PMID:22504960ECO:0000314 direct assay evidence used in manual assertion

Figure 6B.

challenge
unacceptableMOUSE:GABR22012-11-13 15:48:08 CSTGO:0007214 gamma-aminobutyric acid signaling pathway (P)PMID:22504960ECO:0000314 direct assay evidence used in manual assertion

Figure 6B

challenge
unacceptableMOUSE:GABR22012-11-13 15:50:46 CSTGO:0004965 G-protein coupled GABA receptor activity (F)PMID:22504960ECO:0000314 direct assay evidence used in manual assertion

Figure 6B

challenge
unacceptableLACRE:Q5SBN12012-11-17 21:07:28 CSTGO:0046527 glucosyltransferase activity (F)PMID:22361861ECO:0000314 direct assay evidence used in manual assertion

Figure 2, Figure 5

challenge
unacceptableLACRD:A5VL732012-11-17 21:23:44 CSTGO:0046527 glucosyltransferase activity (F)PMID:22361861ECO:0000314 direct assay evidence used in manual assertion

Figure 2, Figure 5

challenge
acceptableRAT:AMYP2012-11-17 21:38:41 CSTGO:0004556 alpha-amylase activity (F)PMID:23007066ECO:0000314 direct assay evidence used in manual assertion

Figure 2

challenge
unacceptableHUMAN:HCST2012-11-17 23:32:29 CSTGO:0035591 signaling adaptor activity (F)PMID:20212116ECO:0000314 direct assay evidence used in manual assertion

Figure 6.

challenge
unacceptableMOUSE:GSK3B2012-11-18 00:02:21 CSTGO:0007188 adenylate cyclase-modulating G-protein coupled receptor signaling pathway (P)PMID:20056751ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2-GSK3β gene deletion reduced urine osmolality after water deprivation. Urine osmolality values of urine collected during 18 hours of water deprivation.

challenge
acceptableMOUSE:GSK3B2012-11-18 01:43:30 CSTGO:0070059 intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress (P)PMID:23028846ECO:0000314 direct assay evidence used in manual assertion

Figure 6

challenge
unacceptableMOUSE:GSK3A2012-11-18 01:40:54 CSTGO:0070059 intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress (P)PMID:23028846ECO:0000314 direct assay evidence used in manual assertion

Figure 6

challenge
unacceptableMOUSE:GSK3B2012-11-18 08:37:56 CSTGO:0032872 regulation of stress-activated MAPK cascade (P)PMID:23028846ECO:0000314 direct assay evidence used in manual assertion

Figure 3. The MAP kinases are regulated by GSK3β inhibition.

challenge
requireschangesMOUSE:GSK3B2012-11-18 08:42:43 CSTGO:0032873 negative regulation of stress-activated MAPK cascade (P)PMID:23028846ECO:0000314 direct assay evidence used in manual assertion

Figure 3. The MAP kinases are regulated by GSK3β inhibition.

challenge
unacceptableMOUSE:GSK3A2012-11-18 08:46:32 CSTGO:0032872 regulation of stress-activated MAPK cascade (P)PMID:23028846ECO:0000314 direct assay evidence used in manual assertion

Figure 3. The MAP kinases are regulated by GSK3β inhibition

challenge
unacceptableMOUSE:GSK3A2012-11-18 08:48:46 CSTGO:0032873 negative regulation of stress-activated MAPK cascade (P)PMID:23028846ECO:0000314 direct assay evidence used in manual assertion

Figure 3. The MAP kinases are regulated by GSK3β inhibition.

challenge
unacceptableMOUSE:GSK3B2012-11-18 08:55:18 CSTGO:0034143 regulation of toll-like receptor 4 signaling pathway (P)PMID:23028846ECO:0000314 direct assay evidence used in manual assertion

Figure 4B and 4C. The inhibition of GSK3β decreased the expression of TLR4 mRNA and TLR4 proteins at 3 hours detected by qRT-PCR.

challenge
requireschangesMOUSE:GSK3B2012-11-18 08:59:30 CSTGO:0034144 negative regulation of toll-like receptor 4 signaling pathway (P)PMID:23028846ECO:0000314 direct assay evidence used in manual assertion

Figure 4B and 4C. The inhibition of GSK3β decreased the expression of TLR4 mRNA and TLR4 proteins at 3 hours detected by qRT-PCR.

challenge
unacceptableMOUSE:GSK3A2012-11-18 09:12:21 CSTGO:0034142 toll-like receptor 4 signaling pathway (P)PMID:23028846ECO:0000314 direct assay evidence used in manual assertion

Figure 4B and 4C. The inhibition of GSK3β decreased the expression of TLR4 mRNA and TLR4 proteins at 3 hours detected by qRT-PCR.

challenge
unacceptableMOUSE:GSK3A2012-11-18 09:15:06 CSTGO:0034144 negative regulation of toll-like receptor 4 signaling pathway (P)PMID:23028846ECO:0000314 direct assay evidence used in manual assertion

Figure 4B and 4C. The inhibition of GSK3β decreased the expression of TLR4 mRNA and TLR4 proteins at 3 hours detected by qRT-PCR.

challenge
unacceptableLACRE:Q5SBN12012-11-18 17:39:45 CSTGO:0004583 dolichyl-phosphate-glucose-glycolipid alpha-glucosyltransferase activity (F)PMID:22361861ECO:0000314 direct assay evidence used in manual assertion

Figure 2, Figure 5

challenge
unacceptableLACRE:Q5SBN12012-11-18 17:41:43 CSTGO:0009011 starch synthase activity (F)PMID:22361861ECO:0000314 direct assay evidence used in manual assertion

Figure 2, Figure 5

challenge
unacceptableLACRE:Q5SBN12012-11-18 17:46:06 CSTGO:0016759 cellulose synthase activity (F)PMID:22361861ECO:0000314 direct assay evidence used in manual assertion

Figure 2, Figure 5

challenge
acceptableLACRE:Q5SBN12012-11-18 17:47:52 CSTGO:0032001 1,4-alpha-glucan 6-alpha-glucosyltransferase activity (F)PMID:22361861ECO:0000314 direct assay evidence used in manual assertion

Figure 2, Figure 5

challenge
unacceptableLACRE:Q5SBN12012-11-18 17:49:32 CSTGO:0033556 dolichyl pyrophosphate Man7GlcNAc2 alpha-1,3-glucosyltransferase activity (F)PMID:22361861ECO:0000314 direct assay evidence used in manual assertion

Figure 2, Figure 5

challenge
unacceptableLACRE:Q5SBN12012-11-18 17:50:57 CSTGO:0033820 DNA alpha-glucosyltransferase activity (F)PMID:22361861ECO:0000314 direct assay evidence used in manual assertion

Figure 2, Figure 5

challenge
unacceptableLACRE:Q5SBN12012-11-18 17:52:09 CSTGO:0033821 DNA beta-glucosyltransferase activity (F)PMID:22361861ECO:0000314 direct assay evidence used in manual assertion

Figure 2, Figure 5

challenge
unacceptableLACRE:Q5SBN12012-11-18 17:53:23 CSTGO:0033822 glucosyl-DNA beta-glucosyltransferase activity (F)PMID:22361861ECO:0000314 direct assay evidence used in manual assertion

Figure 2, Figure 5

challenge
unacceptableLACRE:Q5SBN12012-11-18 17:54:25 CSTGO:0033823 procollagen glucosyltransferase activity (F)PMID:22361861ECO:0000314 direct assay evidence used in manual assertion

Figure 2, Figure 5

challenge
unacceptableLACRE:Q5SBN12012-11-18 17:55:38 CSTGO:0033824 alternansucrase activity (F)PMID:22361861ECO:0000314 direct assay evidence used in manual assertion

Figure 2, Figure 5

challenge
acceptableLACRE:Q5SBN12012-11-18 17:56:50 CSTGO:0033825 oligosaccharide 4-alpha-D-glucosyltransferase activity (F)PMID:22361861ECO:0000314 direct assay evidence used in manual assertion

Figure 2, Figure 5

challenge
unacceptableLACRE:Q5SBN12012-11-18 17:57:52 CSTGO:0033826 xyloglucan 4-glucosyltransferase activity (F)PMID:22361861ECO:0000314 direct assay evidence used in manual assertion

Figure 2, Figure 5

challenge
unacceptableLACRE:Q5SBN12012-11-18 17:58:55 CSTGO:0033828 glucosylglycerol-phosphate synthase activity (F)PMID:22361861ECO:0000314 direct assay evidence used in manual assertion

Figure 2, Figure 5

challenge

acceptable:7
unacceptable:32
requires_changes:5
flagged:0

Annotations challenged by Dimitrios.Spantithos

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
updatedbyinstructorJordan.rausch,
Team Honey Badgers
RAT:ACE2GO:0004180 - carboxypeptidase activity (F)PMID:22595130ECO:0000314 direct assay evidence used in manual assertionchallengeC: 2

0 annotations fixed by Dimitrios.Spantithos