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User:AshFlipp3

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CACAO Spring 2020

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See the documentation at Special:CACAO for more information.

My Annotations

Annotations challenged by AshFlipp3

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history

0 annotations fixed by AshFlipp3

CACAO Fall 2018

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptableDANRE:Q6K1972018-09-11 13:57:06 CDTGO:0007498 mesoderm development (P)PMID:15169890ECO:0000315 mutant phenotype evidence used in manual assertion

FIG:6

Zebra Fish p65

challenge
acceptableHUMAN:HUWE12018-09-18 14:38:11 CDTGO:0098779 positive regulation of mitophagy in response to mitochondrial depolarization (P)PMID:30217973ECO:0000314 direct assay evidence used in manual assertion

Figure 1 and Supplementary Fig. 1- It is positive regulation of mitophagy because HUWE1 depletion impairs AMBRA1-mediated mitophagy. The O/A treatments are indicative of mitochondrial depolarization.

Supplementary Table 1- The Bethyl catalogue number for the HUWE1 antibody tells us that the protein is a human protein.

Organism- Human

challenge
unacceptableCANLF:A0A0C5E1H52018-10-16 12:54:25 CDTGO:1900119 positive regulation of execution phase of apoptosis (P)PMID:25890299ECO:0000314 direct assay evidence used in manual assertion

Fig. 3- Zhangfei causes canine osteosarcoma cells to commit apoptosis.

challenge
unacceptableMOUSE:AQP42018-10-16 14:04:36 CDTGO:0021591 ventricular system development (P)PMID:30293570ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 3 shows that AQP4 plays a key role in filling the ventricles with cerebrospinal fluid. Supplemental Material 4B shows that this was not a result of the systematic arterial blood pressure and that it can be inferred that it is caused by AQP4.

challenge
updatedbyinstructorMOUSE:AQP42018-10-16 14:15:03 CDTGO:0033326 cerebrospinal fluid secretion (P)PMID:30293570ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 5 shows that higher levels of APQ4 can lead to an overloading of CSF in the ventricles since the CSF is not able to flow out of the ventricles. This increases pressure in the ventricles.

Aquaporin-4 as found in uniprot, and AQP4 Mus musculus (Mouse)

challenge
acceptableRABIT:LYSC2018-11-13 11:55:03 CSTGO:0003796 lysozyme activity (F)PMID:37167ECO:0000314 direct assay evidence used in manual assertion

Figure 5 shows the positive linear relationship between the initial rate of lysis of a cell suspension and lysozyme concentration under optimal conditions for rabbit lysozyme. Since the slope is positive, it shows that as the concentration of lysozyme C increases, the rate of lysis also increases. After adding together the total number of calculated amino acid residues found in Table 2, you find that it is 129.4 which is closest to the 130 in the lysozyme C as found on uniprot.

challenge
acceptableCHICK:LYSC2018-11-13 11:57:21 CSTGO:0003796 lysozyme activity (F)PMID:37167ECO:0000314 direct assay evidence used in manual assertion

Figure 5 shows the relative activity between the initial rate of lysis and the concentration of lysozyme in the hen egg white, which represent a linear relationship. Since the slope of the graph is positive, it shows that as the concentration of lysozyme C increases, the rate of lysis will also increase.

challenge
acceptableADVG:CAPSD2018-11-13 14:19:20 CSTGO:0039615 T=1 icosahedral viral capsid (C)PMID:10400786ECO:0000314 direct assay evidence used in manual assertion

Figure 1 shows that there is formation of a capsid with T=1 symmetry. The aleutian mink disease parvovirus strain G (ADVG) capsid protein VP1 self-assembles to form a capsid with the T=1 icosahedral symmetry inside the host cell nucleus. The host in this case are minks. The paper refers to the protein as capsid protein VP2 (strain G), or ADVG-VP2, which is an isoform of of the capsid protein VP1 found in the ADVG.

challenge
acceptableCANLF:P532018-11-13 22:16:27 CSTGO:0045944 positive regulation of transcription by RNA polymerase II (P)PMID:25890299ECO:0000314 direct assay evidence used in manual assertion

Figure 1 shows that cellular tumor antigen p53 (referred to as tumor suppressor protein p53 or just p53 in the paper) used in the experiment is functional within the canine (Canis lupus familiaris or Canis familiaris) osteosarcoma cells. There is a positive regulation of transcription by RNA polymerase II.

challenge
acceptableADVG:NS22018-11-14 22:57:39 CSTGO:0039693 viral DNA genome replication (P)PMID:24606679ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 5 and Figure 6 show that Non-structural protein NS2, or just NS2, is involved in viral genome replication. In the presence of the mutant, pIAMDV(-NS2), the aleutian mink disease parvovirus (strain G) (ADV or AMDV) is unable to replicate its genome within the host cell nucleus. In this experiment, they infected CrFK cells (Crandall feline kidney cell) with the aleutian mink disease parvovirus (AMDV-G) to determine the involvement of NS2 in viral genome replication.

challenge
acceptableADVG:NS12018-11-14 23:09:27 CSTGO:0019034 viral replication complex (C)PMID:24606679ECO:0000314 direct assay evidence used in manual assertion

Figure 4 tells us that Initiator protein NS1, or just NS1, from the aleutian mink disease parvovirus (strain G) (ADV or AMDV) is localized inside the viral DNA replication center of the CrFK host cells (Crandall feline kidney cell).

challenge
acceptableADVG:NS32018-11-15 17:23:18 CSTGO:0042025 host cell nucleus (C)PMID:24606679ECO:0000314 direct assay evidence used in manual assertion

Figure 4 tells us that Non-Structural Protein NS3, or just NS3, from the aleutian mink disease parvovirus (strain G) (ADV or AMDV) is expressed in the host cell nucleus during AMDV replication but is not localized within the viral DNA replication centers. In this experiment, they infected CrFK cells (Crandall feline kidney cell) with the aleutian mink disease parvovirus (AMDV-G) to determine the involvement of NS3 in viral genome replication.

challenge
acceptableHHV8P:CEP22018-11-17 10:19:09 CSTGO:0019033 viral tegument (C)PMID:15613308ECO:0000314 direct assay evidence used in manual assertion

Figure 3 shows that Cytoplasmic envelopment protein 2, or ORF33, is associated with the pelleted tegument-capsid structure of the Human herpesvirus 8 type P (isolate GK18) (HHV-8) (Kaposi's sarcoma-associated herpesvirus). ORF33 is a tegument protein.

challenge
acceptableHHV8P:KITH2018-11-17 10:26:52 CSTGO:0019033 viral tegument (C)PMID:15613308ECO:0000314 direct assay evidence used in manual assertion

Figure 3 shows that Thymidine kinase, or ORF21 (TK ORF21), is associated with the pelleted tegument-capsid structure of the Human herpesvirus 8 type P (isolate GK18) (HHV-8) (Kaposi's sarcoma-associated herpesvirus). ORF21 is a tegument protein.

challenge
acceptableHHV8P:ORF452018-11-17 10:31:04 CSTGO:0019033 viral tegument (C)PMID:15613308ECO:0000314 direct assay evidence used in manual assertion

Figure 3 shows that Protein ORF45, or ORF45, is associated with the pelleted tegument-capsid structure of the Human herpesvirus 8 type P (isolate GK18) (HHV-8) (Kaposi's sarcoma-associated herpesvirus). ORF45 is a tegument protein.

challenge
acceptableHHV8P:LTP2018-11-19 21:58:49 CSTGO:0019030 icosahedral viral capsid (C)PMID:15613308ECO:0000314 direct assay evidence used in manual assertion

Figure 3 shows that Large tegument protein deneddylase, or ORF64, is a tegument protein that is associated with the capsid at one end and attached to the envelope at the other end of the Human herpesvirus 8 type P (isolate GK18) (HHV-8) (Kaposi's sarcoma-associated herpesvirus).

challenge
acceptableHHV8P:LTP2018-11-20 13:48:08 CSTGO:0019031 viral envelope (C)PMID:15613308ECO:0000314 direct assay evidence used in manual assertion

Figure 3 shows that Large tegument protein deneddylase, or ORF64, is a tegument protein that is associated with the capsid at one end and attached to the envelope at the other end of the Human herpesvirus 8 type P (isolate GK18) (HHV-8) (Kaposi's sarcoma-associated herpesvirus).

challenge
acceptableHHV8P:GB2018-11-20 13:48:57 CSTGO:0019031 viral envelope (C)PMID:15613308ECO:0000314 direct assay evidence used in manual assertion

Table 2 shows that Envelope glycoprotein B, or ORF8 (gB ORF8), is an envelope protein that is associated with the viral envelope of the Human herpesvirus 8 type P (isolate GK18) (HHV-8) (Kaposi's sarcoma-associated herpesvirus).

challenge
acceptableHHV8P:GH2018-11-20 13:49:48 CSTGO:0019031 viral envelope (C)PMID:15613308ECO:0000314 direct assay evidence used in manual assertion

Table 2 shows that Envelope glycoprotein H, or ORF22 (gH ORF22), is an envelope protein that is associated with the viral envelope of the Human herpesvirus 8 type P (isolate GK18) (HHV-8) (Kaposi's sarcoma-associated herpesvirus).

challenge
acceptableHHV8P:ORF282018-11-20 13:50:42 CSTGO:0019031 viral envelope (C)PMID:15613308ECO:0000314 direct assay evidence used in manual assertion

Table 2 shows that Protein ORF28, or ORF28, is an envelope protein that is associated with the viral envelope of the Human herpesvirus 8 type P (isolate GK18) (HHV-8) (Kaposi's sarcoma-associated herpesvirus).

challenge
acceptableADVG:NS22018-11-20 13:52:37 CSTGO:0019034 viral replication complex (C)PMID:24606679ECO:0000314 direct assay evidence used in manual assertion

Figure 4 tells us that Non-Structural Protein NS2, or just NS2, from the aleutian mink disease parvovirus (strain G) (ADV or AMDV) is localized inside the viral DNA replication center of the CrFK host cells (Crandall feline kidney cell).

challenge
acceptableHHV8P:GM2018-11-20 13:51:36 CSTGO:0019031 viral envelope (C)PMID:15613308ECO:0000314 direct assay evidence used in manual assertion

Table 2 shows that Envelope glycoprotein M or ORF39, is an envelope protein that is associated with the viral envelope of the Human herpesvirus 8 type P (isolate GK18) (HHV-8) (Kaposi's sarcoma-associated herpesvirus).

challenge

acceptable:19
unacceptable:2
requires_changes:0
flagged:0

Annotations challenged by AshFlipp3

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
unacceptableMckaelaAutumn19,
Team BICH
9CAUD:U5PW98GO:0051260 - protein homooligomerization (P)PMID:29298884ECO:0000314 direct assay evidence used in manual assertionchallengeC: 1

0 annotations fixed by AshFlipp3