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User:AMoreno

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CACAO Spring 2019

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptableCAUVC:PLEC2019-01-29 16:04:16 CSTGO:0000155 phosphorelay sensor kinase activity (F)PMID:16176121ECO:0001202 in vitro protein kinase assay evidence used in manual assertion

In figure 5, phosphotransfer profiling demonstrates phosphorylation of PleD from PleC, a non-motile phage-resistant protein, in Caulobacter crescentus.

challenge
updatedbyinstructorHHV8:Q77UU32019-02-05 15:46:46 CSTGO:0019012 virion (C)PMID:15613308ECO:0007184 protein mass spectrometry evidence used in manual assertion

Figure 1, lane 4 shows that gene ORF8, which codes for Envelope glycoprotein B, is present in Kaposi’s Sarcoma-associated Herpesvirus virion.

challenge
acceptableBPDPK:Q8SD312019-03-19 14:32:02 CDTGO:0098025 virus tail, baseplate (C)PMID:23031178ECO:0005579 immunogold labelling evidence used in manual assertion

In figure 6, the image shows that gp131 of Pseudomonas phage ϕKZ is a component of the base plate of the virion through immune-gold labeling.

Uniprot: PHIKZ131

challenge
acceptable9CAUD:H9C1922019-03-19 15:38:57 CDTGO:0046798 viral portal complex (C)PMID:26887841ECO:0007184 protein mass spectrometry evidence used in manual assertion

Table 1 shows that protein gp42 is a capsid component in Pectobacterium phage ZF40 with a molecular weight of 51.92 kDa as determined through ESI-tandem mass spectrometry. Figure 1D lane 3 shows that the protein was isolated through SDS-PAGE.

Uniprot: ZF40_0042, Putative portal protein

challenge
acceptable9CAUD:H9C1932019-03-19 15:45:44 CDTGO:0098018 viral capsid, minor subunit (C)PMID:26887841ECO:0007184 protein mass spectrometry evidence used in manual assertion

Table 1 shows that protein gp43 is a component of Pectobacterium phage ZF40 with a molecular weight of 36.01 kDa as determined through ESI-tandem mass spectrometry. Figure 1D lane 3 shows that the protein was isolated through SDS-Page.

Uniprot: ZF40_0043, Putative head protein

challenge
acceptable9CAUD:H9C1942019-03-19 15:50:25 CDTGO:0019028 viral capsid (C)PMID:26887841ECO:0007184 protein mass spectrometry evidence used in manual assertion

Table 1 shows that protein gp44 is a capsid component of the Pectobacterium phage ZF40 with a molecular weight of 44.12 kDa as determined through ESI-tandem mass spectrometry. In figure 1D lane 3, it is shown that the protein was isolated through SDS-PAGE.

Uniprot: ZF40_0044, Putative head protein

challenge
acceptable9CAUD:H9C1962019-03-19 15:55:17 CDTGO:0098017 viral capsid, major subunit (C)PMID:26887841ECO:0007184 protein mass spectrometry evidence used in manual assertion

Table 1 shows that protein gp46 is a major capsid protein in the Pectobacterium phage ZF40 with a molecular weight of 34.35 kDa as determined through ESI-tandem mass spectrometry. In figure 1D, lane 3 shows that this protein was isolated through SDS-PAGE.

Uniprot Name(s): Putative major capsid protein, ZF40_0046

challenge
acceptable9CAUD:H9C1982019-03-19 16:00:24 CDTGO:0019012 virion (C)PMID:26887841ECO:0007184 protein mass spectrometry evidence used in manual assertion

Table 1 shows that protein gp48 is a component of the Pectobacterium phage ZF40 with a molecular weight of 18.74 kDa as determined through ESI-tandem mass spectrometry. In figure 1D, lane 3 displays the isolation of the protein using SDS-PAGE.

Uniprot Name(s): Uncharacterized Protein, ZF40_0048

challenge
acceptableASFB7:PP2202019-04-09 15:14:50 CDTGO:0019069 viral capsid assembly (P)PMID:12438573ECO:0006003 electron microscopy evidence used in manual assertion

Figure 8 shows through electron microscopy that the expression of polyprotein pp220 in the African Swine Fever Virus allows for the assembly of coating on the membrane. This can be seen to lead to the formation of a viral core-shell when joined with another polyprotein, pp62.

Uniprot: Polyprotein pp220, Ba71V-92

challenge
acceptableASFB7:PP622019-04-09 15:16:01 CDTGO:0019069 viral capsid assembly (P)PMID:12438573ECO:0006003 electron microscopy evidence used in manual assertion

Figure 8 shows through electron microscopy that the expression of polyprotein pp62 in the African Swine Fever Virus allows for the assembly of shell-like membrane, when it is joined with the presence of polyprotein pp220. Figure 8 also shows the interaction of the two polyproteins to form this shell through the use of gold-labelling.

Uniprot: Polyprotein pp62, Ba71V-94

challenge
acceptableASFB7:VHLP2019-04-09 16:02:37 CDTGO:0019012 virion (C)PMID:30185597ECO:0001582 liquid chromatography coupled with tandem mass spectrometry evidence used in manual assertion

The protein characterized by ORF A104R in the African Swine Fever Virus, strain Ba71V, was identified as a component of the virion through liquid chromatography with tandem mass spectrometry as shown in table 1.

Uniprot: BA71V-033, Viral histone-like protein

challenge
acceptableASFB7:H962L2019-04-09 16:00:58 CDTGO:0019012 virion (C)PMID:30185597ECO:0001582 liquid chromatography coupled with tandem mass spectrometry evidence used in manual assertion

The protein characterized by ORF B962L in the African Swine Fever Virus, strain Ba71V, was identified as a component of the virion through liquid chromatography with tandem mass spectrometry, as shown in table 1.

Uniprot: Putative RNA helicase B962L, Ba71V-072

challenge
acceptableASFB7:VF1132019-04-09 16:07:55 CDTGO:0019012 virion (C)PMID:30185597ECO:0001582 liquid chromatography coupled with tandem mass spectrometry evidence used in manual assertion

The protein characterized by ORF D1133L in the African Swine Fever Virus, strain Ba71V, was identified as a component of the virion through liquid chromatography with tandem mass spectrometry, as shown in table 1.

Uniprot: Putative ATP-dependent RNA helicase D1133L, Ba71V-106

challenge
acceptableASFB7:RPB12019-04-09 16:13:28 CDTGO:0019012 virion (C)PMID:30185597ECO:0001582 liquid chromatography coupled with tandem mass spectrometry evidence used in manual assertion

The protein characterized by ORF NP1450L in the African Swine Fever Virus, strain Ba71V, was identified as a component of the virion through liquid chromatography with tandem mass spectrometry, as shown in table 1.

Uniprot: Probable DNA-directed RNA polymerase subunit 1 homolog, Ba71V-99

challenge
acceptableASFB7:P172019-04-09 16:19:53 CDTGO:0019012 virion (C)PMID:30185597ECO:0001582 liquid chromatography coupled with tandem mass spectrometry evidence used in manual assertion

The protein characterized by ORF D117L in the African Swine Fever Virus, strain Ba71V, was isolated by SDS-PAGE in figure 1B and noted as p17. It was then identified as a component of the virion through liquid chromatography with tandem mass spectrometry, as shown in table 1.

Uniprot: Major structural protein p17, Ba71V-107

challenge
acceptableASFB7:VF2492019-04-09 17:01:49 CDTGO:0019012 virion (C)PMID:30185597ECO:0001582 liquid chromatography coupled with tandem mass spectrometry evidence used in manual assertion

The protein characterized by ORF M1249L in the African Swine Fever Virus, strain Ba71V was identified as a component of the virion through liquid chromatography with tandem mass spectrometry, as shown in table 1.

Uniprot: Uncharacterized protein M1249L, Ba71V-060

challenge
acceptableASFB7:VF1232019-04-09 17:05:46 CDTGO:0019012 virion (C)PMID:30185597ECO:0001582 liquid chromatography coupled with tandem mass spectrometry evidence used in manual assertion

The protein characterized by ORF CP123L in the African Swine Fever Virus, strain Ba71V was identified as a component of the virion through liquid chromatography with tandem mass spectrometry, as shown in table 1.

Uniprot: Uncharacterized protein CP123L, Ba71V-91

challenge
updatedbyinstructorASFB7:PP622019-04-09 17:27:15 CDTGO:0019012 virion (C)PMID:30185597ECO:0001582 liquid chromatography coupled with tandem mass spectrometry evidence used in manual assertion

The protein characterized by ORF CP530R and p15 in the African Swine Fever Virus, strain Ba71V, was identified as a component of the virion through liquid chromatography with tandem mass spectrometry, as shown in table 1. This protein is a product of the cleavage of polyprotein 62 and therefore is labelled under it.

Uniprot: Polyprotein pp62, Ba71V-94 p15/p35/p8 proteins from the polypeptide of CP530R (which is pp62)

challenge
acceptableASFB7:RPB22019-04-09 17:34:22 CDTGO:0019012 virion (C)PMID:30185597ECO:0001582 liquid chromatography coupled with tandem mass spectrometry evidence used in manual assertion

The protein characterized by ORF EP1242L in the African Swine Fever Virus, strain Ba71V, was identified as a component of the virion through liquid chromatography with tandem mass spectrometry, as shown in table 1.

Uniprot: DNA-directed RNA polymerase subunit 2, Ba71V-053

challenge
acceptableASFB7:VF1292019-04-09 17:40:17 CDTGO:0019012 virion (C)PMID:30185597ECO:0001582 liquid chromatography coupled with tandem mass spectrometry evidence used in manual assertion

The protein characterized by ORF C129R in the African Swine Fever Virus, strain Ba71V, was identified as a component of the virion through liquid chromatography with tandem mass spectrometry, as shown in table 1.

Uniprot: Uncharacterized Protein C129R, Ba71V-062

challenge
acceptableASFB7:VF7172019-04-09 17:46:44 CDTGO:0019012 virion (C)PMID:30185597ECO:0001582 liquid chromatography coupled with tandem mass spectrometry evidence used in manual assertion

The protein characterized by ORF C717R in the African Swine Fever Virus, strain Ba71V, was identified as a component of the virion through liquid chromatography with tandem mass spectrometry, as shown in table 1.

Uniprot: Uncharacterized Protein C717R, Ba71V-064

challenge
acceptableASFB7:VF1772019-04-09 17:49:24 CDTGO:0019012 virion (C)PMID:30185597ECO:0001582 liquid chromatography coupled with tandem mass spectrometry evidence used in manual assertion

The protein characterized by ORF I177L in the African Swine Fever Virus, strain Ba71V, was identified as a component of the virion through liquid chromatography with tandem mass spectrometry, as shown in table 1.

Uniprot: Membrane protein I177L, BA71V-145

challenge
acceptableASFB7:VF1452019-04-09 17:53:14 CDTGO:0019012 virion (C)PMID:30185597ECO:0001582 liquid chromatography coupled with tandem mass spectrometry evidence used in manual assertion

The protein characterized by ORF K145R in the African Swine Fever Virus, strain Ba71V, was identified as a component of the virion through liquid chromatography with tandem mass spectrometry, as shown in table 1.

Uniprot: Uncharacterized protein K145R, Ba71V-051

challenge
acceptableASFB7:E248R2019-04-09 17:58:43 CDTGO:0019012 virion (C)PMID:30185597ECO:0001582 liquid chromatography coupled with tandem mass spectrometry evidence used in manual assertion

The protein characterized by ORF E248R in the African Swine Fever Virus, strain Ba71V, was identified as a component of the virion through liquid chromatography with tandem mass spectrometry, as shown in table 1.

Uniprot: Protein E248R, Ba71V-132

challenge
acceptableASFB7:VF7062019-04-09 18:03:20 CDTGO:0019012 virion (C)PMID:30185597ECO:0001582 liquid chromatography coupled with tandem mass spectrometry evidence used in manual assertion

The protein characterized by ORF Q706L in the African Swine Fever Virus, strain Ba71V, was identified as a component of the virion through liquid chromatography with tandem mass spectrometry, as shown in table 1.

Uniprot: Putative ATP-dependent RNA helicase Q706L, Ba71V-122

challenge
acceptableASFB7:VF4212019-04-13 08:58:55 CDTGO:0019012 virion (C)PMID:30185597ECO:0001582 liquid chromatography coupled with tandem mass spectrometry evidence used in manual assertion

The protein characterized by ORF K421R in the African Swine Fever Virus, strain Ba71V, was identified as a component of the virion through liquid chromatography with tandem mass spectrometry, as shown in table 1.

Uniprot: Uncharacterized protein K421R, Ba71V-052

challenge

acceptable:24
unacceptable:0
requires_changes:0
flagged:0

Annotations challenged by AMoreno

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
updatedbyinstructorRodrigzma3,
Ca$h Money Curators
9CAUD:H6X3M8GO:0019012 - virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertionchallengeC: 1

fixed by AMoreno
updatedbyinstructorRodrigzma3,
Ca$h Money Curators
9CAUD:H6X3K8GO:0019012 - virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertionchallengeC: 1
updatedbyinstructorNavick,
Ca$h Money Curators
9CAUD:A0A2P1CKP5GO:0019012 - virion (C)PMID:30366363ECO:0007184 protein mass spectrometry evidence used in manual assertionchallengeC: 1

fixed by AMoreno
updatedbyinstructorRodrigzma3,
Ca$h Money Curators
9CAUD:A0A2P1CKJ3GO:0019012 - virion (C)PMID:30366363ECO:0007184 protein mass spectrometry evidence used in manual assertionchallengeC: 1

fixed by AMoreno
updatedbyinstructorNavick,
Ca$h Money Curators
9CAUD:A0A2P1CKR0GO:0019012 - virion (C)PMID:30366363ECO:0007184 protein mass spectrometry evidence used in manual assertionchallengeC: 1

fixed by AMoreno
updatedbyinstructorNavick,
Ca$h Money Curators
9CAUD:A0A2P1CL06GO:0019012 - virion (C)PMID:30366363ECO:0007184 protein mass spectrometry evidence used in manual assertionchallengeC: 1

fixed by AMoreno
acceptableFFitzgerald,
Ca$h Money Curators
9CAUD:H6X4Y3GO:0019012 - virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertionchallengeC: 2
acceptableFFitzgerald,
Ca$h Money Curators
9CAUD:H6X4Z1GO:0019012 - virion (C)PMID:23593293ECO:0007184 protein mass spectrometry evidence used in manual assertionchallengeC: 1

5 annotations fixed by AMoreno