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TANCI:H6U1I8

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Species (Taxon ID) Tanacetum cinerariifolium (Dalmatian daisy) (Chrysanthemumcinerariifolium). (118510)
Gene Name(s) GLIP (ECO:0000313 with EMBL:AEZ63357.1)
Protein Name(s) GDSL lipase-like protein (ECO:0000313 with EMBL:AFJ04755.1)

Type III-1 GDSL lipase (ECO:0000313 with EMBL:AEZ63357.1) Type III-2 GDSL lipase (ECO:0000313 with EMBL:AEZ63358.1)

External Links
UniProt H6U1I8
EMBL JN418994
JN418995
JQ513384
JX913533
ProteinModelPortal H6U1I8
GO GO:0016788
GO:0006629
Gene3D 3.40.50.1110
InterPro IPR001087
IPR013831
Pfam PF00657

Annotations

Qualifier GO ID GO term name Reference ECO ID ECO term name with/from Aspect Extension Notes Status

enables

GO:0016412

serine O-acyltransferase activity

PMID:22385412[1]

ECO:0000315

mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete

GO:0016412

serine O-acyltransferase activity

PMID:22385412[1]

ECO:0000315

F

GDSL lipase was purified and recombineered into E. coli with a maltose binding protein fusion. The reaction transfers the chrysanthemoyl group from coenzyme A to pyrethrolone. Figure 3 shows mutations in serine of the GDSL domain eliminate acetyltransferase activity and maltose binding protein alone does not have acetyltransferase activity.

complete
CACAO 9773

enables

GO:0016788

hydrolase activity, acting on ester bonds

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR001087

F

Seeded From UniProt

complete

Notes

References

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  1. 1.0 1.1 Kikuta, Y et al. (2012) Identification and characterization of a GDSL lipase-like protein that catalyzes the ester-forming reaction for pyrethrin biosynthesis in Tanacetum cinerariifolium- a new target for plant protection. Plant J. 71 183-93 PubMed GONUTS page