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GO:0005674transcription factor TFIIF complexPMID:7995524IDA: Inferred from Direct Assay C
This annotation made on page: YEAST:TAF14
By: Bmanieri (group Team Chicken Day) on 2014-01-30 10:05:52 CST.




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Entry TypeChallenging User,GroupTime/DateChallenge ReasonPoints/Assessment
ChallengeMichelel,
MichSt14A 16
2014-04-10 21:57:30 CDT

A better GO term for this article is GO:0005674, transcription factor TFIIF complex. IDA is still okay to use. Figure 4A shows that the protein Tfg3 is a component of TFIIF because it comigrates with components of TFIIF complex, and Figure 4B shows that when the complex is probed with anti-Tfg3 antibodies, there is a reaction showing that Tfg3 is present in the complex. Figure 4C-E shows that Tfg2 associates with common binding factors also part of the TFIIF complex, again by immunoprecipitation and antibody probing. Sequence homology does not prove that Tfg3 associates with the complex.

Table 1 also shows via IMP/IDA that when tfg3 is deleted, there is significantly less transcription compared to wild type cells because the TFIIF complex cannot form correctly, and transcription is impacted.

0
ChallengeGrolfe,
Team Pancakes At Nite
2014-02-06 15:31:08 CST

The evidence code for this is wrong. It should be ISA: Inferred from Sequence Alignment.

1
Private
Assessment
JimHu2014-04-19 19:52:07 CDTYou need to be an instructor to view these notes.Unacceptable
Unique/Original
Public
Assessment
DanielRenfro2014-04-10 21:57:30 CDT

This annotation has been flagged because it has been edited since last assessment ||GO:0005674||transcription factor TFIIF complex||PMID:7995524||IDA: Inferred from Direct Assay||||C||Figure 5 Depicts region 4.2 showing the sequence homology between Tfg2 of bacterial cr factors. Alignment of the carboxy-terminal residues of Tfg2 with the carboxy-terminal residues of E. coli and Bacillus subtilis.||complete

Flagged
Private
Assessment
Suzialeksander2014-02-18 13:52:06 CSTYou need to be an instructor to view these notes.Requires Changes
Protein
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