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GO:0008940nitrate reductase activityPMID:1922075IDA: Inferred from Direct Assay F
This annotation made on page: EMENI:NIRA
By: Jolive15 (group Team Denitrifiers) on 2011-10-22 22:02:13 CDT.




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Entry TypeChallenging User,GroupTime/DateChallenge ReasonPoints/Assessment
ChallengeLrodgers,
Team KremlinOnTheCrum
2011-10-29 09:37:56 CDT

Figure 5 shows that nirA transcription is constitutive. It does not show that it is involved in nitrate reductase activity. Instead what the paper shows is that nirA is acting as a transcription factor for niaD and niiA. This in turn regulates the steady state concentration of nitrate and nitrite reductase mRNA concentration.

6
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Assessment
Mkline2012-03-28 12:53:45 CDTYou need to be an instructor to view these notes.Acceptable
Public
Assessment
Bmcintosh2011-11-18 16:56:02 CST

We agree with the challenge - the experiment does not characterize the activity of the protein, only the mRNA message by Northern blotting. The changes in expression are assessed in various NirA mutants.

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