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Cacao

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GO:0008800beta-lactamase activityPMID:21238457IMP: Inferred from Mutant Phenotype F
This annotation made on page: STRCL:BLIP
By: ORichter (group Team Purple B 2018) on 2018-04-15 20:27:30 CDT.




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Entry TypeChallenging User,GroupTime/DateChallenge ReasonPoints/Assessment
Public
Assessment		Ivanerill2018-05-07 08:11:49 CDTNo notes given.Acceptable
✔ Protein
✔ Publication
✔ Qualifier
✔ Go term
✔ Evidence
✔ With/From
✔ Notes
✔ Unique/Original
Public
Assessment		DanielRenfro2018-05-02 16:19:22 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0033252 regulation of beta-lactamase activity PMID:21238457 IMP: Inferred from Mutant Phenotype P Figure 3 shows the interaction between K74G BLIP (a beta-lactamase inhibitory protein mutant) with S. aureus PC1 wild-type β-lactamase and its A104E (beta-lactamase) mutant. K74G BLIP has greater inhibitory effect on the wild type beta-lactamase than the mutant beta-lactamase. Table 2 includes the inhibition constant of the purified K74G BLIP variant for PC1 β-lactamase, which shows about a 10-fold increase in comparison to that of wild type BLIP. complete
CACAO 13272
on STRCL:BLIP
Flagged
Public
Assessment		DanielRenfro2018-05-02 14:54:38 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0005576 extracellular region PMID:21238457 IMP: Inferred from Mutant Phenotype C Figure 3 shows the interaction between K74G BLIP (beta-lactamase inhibitory protein) with S. aureus PC1 wild-type β-lactamase and its A104E mutant. Inhibition of the wild-type beta-lactamase was greater in comparison to mutant A104E in the presence of K74G BLIP. Table 2 shows a lesser inhibition constant for wt PC1 with K74G BLIP compared to A104E mutant PC1 with K74G. complete
CACAO 13272
on STRCL:BLIP
Flagged
Public
Assessment		Ivanerill2018-05-02 10:04:18 CDT

the annotation is now to STRCL:BLIP. I don't believe there is evidence in the manuscript supporting that this gene has GO:0008800, beta-lactamase activity. To quote your notes: "Inhibition of the wild-type beta-lactamase was greater in comparison to mutant A104E in the presence of K74G BLIP"

Requires Changes
✗ Go term
Public
Assessment		Ivanerill2018-04-30 10:32:49 CDT

Move Annotation 13272 from STAAU:BLAC to STRCL:BLIP

Updated by Instructor
Public
Assessment		Ivanerill2018-04-30 08:24:58 CDT

This annotation is on the wrong protein, using the wrong GO term...

Requires Changes
✗ Protein
✔ Publication
✔ Qualifier
✗ Go term
✔ Evidence
✔ With/From
✗ Notes
✔ Unique/Original


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