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Cacao

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GO:0003796lysozyme activityPMID:15058185IDA: Inferred from Direct Assay F
This annotation made on page: 9CAUD:Q7Y4H8
By: MKoert (group Team Red A 2018) on 2018-03-01 12:58:33 CST.



TeamPoints
Team Red A 20185




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Entry TypeChallenging User,GroupTime/DateChallenge ReasonPoints/Assessment
Public
Assessment
Ivanerill2018-04-15 14:07:54 CDTNo notes given.Acceptable
Protein
Publication
Qualifier
Go term
Evidence
With/From
Notes
Unique/Original
Public
Assessment
DanielRenfro2018-03-23 10:41:48 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0061783 peptidoglycan muralytic activity PMID:15058185 IDA: Inferred from Direct Assay F Expression and activity of lytic enzyme was confirmed in Figures 2A/B through SDS Page and an in situ assay. Figure A showed expression of lytic enzyme in ecoli vector plasmid. Figure B showed lytic activity corresponding to the molar mass of a 42kDA protein. complete
CACAO 13084
on 9CAUD:Q7Y4H8
Flagged
Public
Assessment
Ivanerill2018-03-08 15:32:07 CST

Agree with Dr. Caruso. Also note that other teams are working on the same paper. If colluding, first correct annotation matching GO term, evidence code, paper gets the credit.


Requires Changes
Protein
Publication
Qualifier
Go term
Evidence
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Notes
Unique/Original
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Assessment
Scaruso2018-03-08 10:23:16 CST

Lysozyme activity is too specific a GO term, the authors have not shown that activity. Note in the introduction of the paper: Up to date, at least 4 distinct types of endolysins have been identified: lysozymes (EC 3.2.1.17; ‘muramidases’), transglycosidases (EC 3.2.1), amidases (EC 3.5.1.4) and endopeptidases (EC 3.4.21). Here they have shown the endolysin activity by showing the purified protein degraded the cell wall, but not which kind of lysin it is. It could be any of the four known (or other unknown) types. You should back up a step in specificity. The notes are good, but perhaps a tad more detail would be nice.

Requires Changes
Protein
Publication
Qualifier
Go term
Evidence
With/From
Notes
Unique/Original