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GO:0004003ATP-dependent DNA helicase activityPMID:28009009IMP: Inferred from Mutant Phenotype F
This annotation made on page: BPT5:HEL10
By: Jknapo1 (group Team Red-A) on 2017-04-05 10:33:09 CDT.



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Team Red-A0




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Entry TypeChallenging User,GroupTime/DateChallenge ReasonPoints/Assessment
Private
Assessment
Ivanerill2017-05-25 05:23:58 CDTYou need to be an instructor to view these notes.Updated by Instructor
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Ivanerill2017-05-24 06:38:06 CDT

What Fig. 1d shows is GO:0016887 ATPase activity, or better yet, GO:0008094 DNA-dependent ATPase activity, not ATP-dependent DNA helicase activity. This had already been mentioned in previous assessments.

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DanielRenfro2017-04-17 09:43:03 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0004003 ATP-dependent DNA helicase activity PMID:28009009 IMP: Inferred from Mutant Phenotype F Figure 1b shows the mass of the eluted molecules to be consistent with those in DNA-dependent ATPase activity. Figure 1c shows enzymatic activity was only stimulated in the presence of duplex DNA. Figure 1d shows after cloning of D10 protein, D10-R389N (motif VI) was expressed and purified. When compared to wild type, a point mutation of the motif stopped all DNA-dependent ATPase activity. complete
CACAO 12443
on BPT5:HEL10
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Public
Assessment
Ivanerill2017-04-15 19:08:53 CDT

Why not GO:0008094? Where is the evidence for term? There is other evidence for ATPase act. in same figure.

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