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Cacao

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GO:0004748ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptorPMID:8969495IMP: Inferred from Mutant Phenotype F
This annotation made on page: BACSU:RIR1
By: Sjesse (group TeamRed) on 2015-04-06 10:55:09 CDT.



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Entry TypeChallenging User,GroupTime/DateChallenge ReasonPoints/Assessment
Private
Assessment		Sefa12015-05-13 14:56:50 CDTYou need to be an instructor to view these notes.Acceptable
✔ Protein
✔ Publication
✔ Qualifier
✔ Go term
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✔ Notes
✔ Unique/Original
Public
Assessment		DanielRenfro2015-05-10 14:30:14 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0004748 ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor PMID:8969495 IMP: Inferred from Mutant Phenotype F This paper follows the studies in another article (PMID 4627024) which I don't have access to read past the beginning results and the abstract. This previous article used a temperature sensitive mutant ts-167 to determine the function of the nrdEF locus. That paper determined the function by analyzing the pool of dNTP present at the restrictive temperature. dCTP and dATP were not apparent at these restrictive temperatures.

The reference listed above (PMID 8969495) used phylogeny and sequencing to determine that Enterobacteria favor the use of nrdAB loci, whereas Bacilus subtils has evolved to use nrdEF. They also used clone pMP25.8 to rescue the temperature sensitive mutant, and later sequenced the nrdE mutant to determine there were 2 neutral mutations and 1 mutation that led to the lethality of the temperature sensitive mutant. They concluded that this mutation was the cause for the loss of function of the NrdE protein.

The mutant lethality due to the loss of dCTP and dATP synthesis provides evidenct that this gene is responsible for the essential enzyme responsible for reductase activity for the respective ribonucleoside-diphosphates. ||complete
CACAO 10873

on BACSU:RIR1
Flagged
Public
Assessment		Sefa12015-05-03 22:38:22 CDT

Notes are not very clear. Why did the authors transform plasmid pJM606 into strain PB1679? Are all these 3 mutations affect the function of the protein?

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✔ Protein
✔ Publication
✔ Qualifier
✔ Go term
✔ Evidence
✔ Notes
✔ Unique/Original


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