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GO:0061630ubiquitin protein ligase activityPMID:11807090IDA: Inferred from Direct Assay F
This annotation made on page: XENLA:CHFR
By: Lhicks (group Team Ubiquitous Mafia) on 2015-02-05 18:23:32 CST.




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Entry TypeChallenging User,GroupTime/DateChallenge ReasonPoints/Assessment
ChallengeMin.lorna,
Team Ood
2015-03-17 08:12:43 CDT

Protein should be Q96EP1. Notes: Fig 1A: Chfr immunoprecipitates contain ubiquitin and promote the formation of large ubiquitinated proteins when incubated with E1 and Ubc4 (an E2 enzyme) --Fig 1C: E1, Ubc4, and Chfr were incubated. Chfr was found to auto-ubiquinate - ubiquinate ligases containing a ring finger domain are known to auto-ubiquinate, and Chfr has a ring finger domain --Fig 8A: Plk1 and Chfr were incubated for varying amounts of time. The amount and size of ubiquinated Plk1 increased as incubation time increased, indicating that Chfr causes Plk1 ubiquination.

9
ChallengeKsenagbe,
Team Mic Drop
2015-03-11 20:15:42 CDT

Describe what is seen in Figure 6

None, yet.
Private
Assessment
Amcmillan2015-03-25 19:33:35 CDTYou need to be an instructor to view these notes.Corrected Through Challenges
Protein
Publication
Qualifier
Go term
Evidence
With/From
Notes
Unique/Original
Public
Assessment
DanielRenfro2015-03-17 08:12:43 CDT

This annotation has been flagged because it has been challenged since last assessment

Flagged
Public
Assessment
DanielRenfro2015-03-11 20:15:42 CDT

This annotation has been flagged because it has been challenged since last assessment

Flagged
Private
Assessment
Amcmillan2015-02-22 15:32:16 CSTYou need to be an instructor to view these notes.Requires Changes
Protein
Publication
Qualifier
Go term
Evidence
With/From
Notes