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SGD:APN1

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Species (Taxon ID) Saccharomyces cerevisiae (baker's yeast) (taxon:4932)
Gene Name(s) APN1 ( synonyms: YKL114C, DNA-(apurinic or apyrimidinic site) lyase APN1 )
Protein Name(s) Major apurinic/apyrimidinic endonuclease,
External Links
SGD S000001597

Annotations

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0003677

DNA binding

SGD_REF:S000124036

IEA: Inferred from Electronic Annotation

InterPro:IPR001719
InterPro:IPR018246

F

From SGD

GO:0003906

DNA-(apurinic or apyrimidinic site) lyase activity

SGD_REF:S000039558
PMID:1693433[1]

IMP: Inferred from Mutant Phenotype

F

From SGD

GO:0003906

DNA-(apurinic or apyrimidinic site) lyase activity

SGD_REF:S000039558
PMID:1693433[1]

ISS: Inferred from Sequence or Structural Similarity

F

From SGD

GO:0003906

DNA-(apurinic or apyrimidinic site) lyase activity

SGD_REF:S000073336
PMID:12767225[2]

IDA: Inferred from Direct Assay

F

From SGD

GO:0003906

DNA-(apurinic or apyrimidinic site) lyase activity

SGD_REF:S000124037

IEA: Inferred from Electronic Annotation

EC:4.2.99.18

F

From SGD

GO:0004519

endonuclease activity

SGD_REF:S000124036

IEA: Inferred from Electronic Annotation

InterPro:IPR018246

F

From SGD

GO:0005622

intracellular

SGD_REF:S000124036

IEA: Inferred from Electronic Annotation

InterPro:IPR018246
InterPro:IPR001719

C

From SGD

GO:0005634

nucleus

SGD_REF:S000050884
PMID:7690756[3]

IDA: Inferred from Direct Assay

C

From SGD

GO:0005634

nucleus

SGD_REF:S000148669

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0539

C

From SGD

GO:0005634

nucleus

SGD_REF:S000148671

IEA: Inferred from Electronic Annotation

UniProtKB-SubCell:SL-0191

C

From SGD

GO:0005739

mitochondrion

SGD_REF:S000060116
PMID:11238901[4]

IDA: Inferred from Direct Assay

C

From SGD

GO:0006281

DNA repair

SGD_REF:S000124036

IEA: Inferred from Electronic Annotation

InterPro:IPR018246
InterPro:IPR001719

P

From SGD

GO:0006281

DNA repair

SGD_REF:S000148669

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0234

P

From SGD

GO:0006284

base-excision repair

SGD_REF:S000051442
PMID:9295360[5]

IDA: Inferred from Direct Assay

P

From SGD

GO:0006974

response to DNA damage stimulus

SGD_REF:S000148669

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0227

P

From SGD

GO:0008081

phosphoric diester hydrolase activity

SGD_REF:S000052264
PMID:3056935[6]

IDA: Inferred from Direct Assay

F

From SGD

GO:0008270

zinc ion binding

SGD_REF:S000124036

IEA: Inferred from Electronic Annotation

InterPro:IPR001719
InterPro:IPR018246

F

From SGD

GO:0008311

double-stranded DNA specific 3'-5' exodeoxyribonuclease activity

SGD_REF:S000082522
PMID:16024777[7]

IDA: Inferred from Direct Assay

F

From SGD

GO:0016829

lyase activity

SGD_REF:S000148669

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0456

F

From SGD

GO:0017005

3'-tyrosyl-DNA phosphodiesterase activity

SGD_REF:S000072272
PMID:12397185[8]

IDA: Inferred from Direct Assay

F

From SGD

GO:0046872

metal ion binding

SGD_REF:S000148669

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0479

F

From SGD


Notes

References

See Help:References for how to manage references in GONUTS.
  1. 1.0 1.1 Popoff, SC et al. (1990) Yeast structural gene (APN1) for the major apurinic endonuclease: homology to Escherichia coli endonuclease IV. Proc. Natl. Acad. Sci. U.S.A. 87 4193-7 PubMed GONUTS page
  2. Jilani, A et al. (2003) Characterization of two independent amino acid substitutions that disrupt the DNA repair functions of the yeast Apn1. Biochemistry 42 6436-45 PubMed GONUTS page
  3. Ramotar, D et al. (1993) Intracellular localization of the Apn1 DNA repair enzyme of Saccharomyces cerevisiae. Nuclear transport signals and biological role. J. Biol. Chem. 268 20533-9 PubMed GONUTS page
  4. Vongsamphanh, R et al. (2001) Pir1p mediates translocation of the yeast Apn1p endonuclease into the mitochondria to maintain genomic stability. Mol. Cell. Biol. 21 1647-55 PubMed GONUTS page
  5. Wang, Z et al. (1997) Molecular mechanism of base excision repair of uracil-containing DNA in yeast cell-free extracts. J. Biol. Chem. 272 24064-71 PubMed GONUTS page
  6. Johnson, AW & Demple, B (1988) Yeast DNA diesterase for 3'-fragments of deoxyribose: purification and physical properties of a repair enzyme for oxidative DNA damage. J. Biol. Chem. 263 18009-16 PubMed GONUTS page
  7. Ishchenko, AA et al. (2005) The 3'->5' exonuclease of Apn1 provides an alternative pathway to repair 7,8-dihydro-8-oxodeoxyguanosine in Saccharomyces cerevisiae. Mol. Cell. Biol. 25 6380-90 PubMed GONUTS page
  8. Liu, C et al. (2002) Repair of topoisomerase I covalent complexes in the absence of the tyrosyl-DNA phosphodiesterase Tdp1. Proc. Natl. Acad. Sci. U.S.A. 99 14970-5 PubMed GONUTS page
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