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PMID:9933595

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Citation

Mahon, MG, Lindstedt, KA, Hermann, M, Nimpf, J and Schneider, WJ (1999) Multiple involvement of clusterin in chicken ovarian follicle development. Binding to two oocyte-specific members of the low density lipoprotein receptor gene family. J. Biol. Chem. 274:4036-44

Abstract

The interaction of the female germ cell with somatic cells during the development of the ovarian follicle in the chicken provides a prime system to study gene expression. Here, we have uncovered the involvement of clusterin, the function(s) of which is still poorly understood, in this complex process. As revealed by molecular cloning, chicken clusterin is a 428-residue protein that migrates at 70 kDa on SDS-polyacrylamide gel electrophoresis and possesses most of the structural features of its mammalian successors. However, in contrast to mammalian clusterin, the chicken protein appears not to be cleaved intracellularly into a disulfide-linked heterodimer; possibly as a consequence thereof, it is not secreted constitutively and is absent from the circulation, where most of clusterin is found in mammals. In the ovary, clusterin is a major product of the somatic granulosa cells, in a pattern correlating with the developmental phases of individual follicles. In that, transcript levels are high not only at onset of vitellogenesis, but also in atretic follicles and in the postovulatory follicle sac, i.e. in situations characterized by apoptotic events. Yolk of growing oocytes contains a 43-kDa truncated form of clusterin that does not appear to be synthesized within the oocyte. Rather, we here show for the first time that 70-kDa clusterin interacts not only with megalin, but also with two chicken oocyte-specific members of the low density lipoprotein receptor (LDLR) gene family. These receptors, termed LDLR-related protein with eight ligand binding repeats (LR8) and LDLR-related protein (380 kDa), likely internalize granulosa cell-derived 70-kDa clusterin, which may subsequently be processed to the 43-kDa product. Thus, chicken clusterin could serve as a marker for follicular atresia and resorption, and, based on its ability to bind several other proteins, it may serve as carrier for the receptor-mediated endocytosis into oocytes of components important for embryonic development, two hitherto unknown functions of this intriguing protein.

Links

PubMed

Keywords

Amino Acid Sequence; Animals; Base Sequence; Chickens; Clusterin; Consensus Sequence; DNA/metabolism; Female; Glycoproteins/chemistry; Glycoproteins/genetics; Glycoproteins/physiology; Glycosylation; Humans; Molecular Chaperones; Molecular Sequence Data; Molecular Weight; Oocytes/growth & development; Ovarian Follicle/growth & development; Quail; RNA, Messenger/metabolism; Receptors, LDL/genetics; Sequence Alignment

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CHICK:Q6PTX2

located_in

GO:0060417: yolk

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:LRP8

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q9YGP0

F

Seeded From UniProt

complete

CHICK:Q9YGP0

located_in

GO:0060417: yolk

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:Q9YGP0

located_in

GO:0005634: nucleus

ECO:0000303: author statement without traceable support used in manual assertion

C

Seeded From UniProt

complete

CHICK:Q6PTX2

part_of

GO:0060417: yolk

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:Q9YGP0

involved_in

GO:0001552: ovarian follicle atresia

ECO:0000303: author statement without traceable support used in manual assertion

P

Seeded From UniProt

complete

CHICK:Q9YGP0

part_of

GO:0005634: nucleus

ECO:0000303: author statement without traceable support used in manual assertion

C

Seeded From UniProt

complete

CHICK:Q9YGP0

part_of

GO:0060417: yolk

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:Q9YGP0

enables

GO:0050750: low-density lipoprotein particle receptor binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q98931

F

Seeded From UniProt

complete

CHICK:Q9YGP0

involved_in

GO:0001541: ovarian follicle development

ECO:0000303: author statement without traceable support used in manual assertion

P

Seeded From UniProt

complete


See also

References

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