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PMID:9914480
Citation |
De Cock, H, Schäfer, U, Potgeter, M, Demel, R, Müller, M and Tommassen, J (1999) Affinity of the periplasmic chaperone Skp of Escherichia coli for phospholipids, lipopolysaccharides and non-native outer membrane proteins. Role of Skp in the biogenesis of outer membrane protein. Eur. J. Biochem. 259:96-103 |
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Abstract |
The Skp protein of Escherichia coli has been proposed to be a periplasmic molecular chaperone involved in the biogenesis of outer membrane proteins. In this study, evidence is obtained that Skp exists in two different states characterized by their different sensitivity to proteases. The conversion between these states can be modulated in vitro by phospholipids, lipopolysaccharides and bivalent cations. Skp is able to associate with and insert into phospholipid membranes in vitro, indicating that it may associate with phospholipids in the inner and/or outer membrane in vivo. In addition, it interacts specifically with outer membrane proteins that are in their non-native state. We propose that Skp is required in vivo for the efficient targeting of unfolded outer membrane proteins to the membrane. |
Links | |
Keywords |
Bacterial Outer Membrane Proteins/metabolism; DNA-Binding Proteins/metabolism; Endopeptidases/metabolism; Escherichia coli/metabolism; Escherichia coli Proteins; Lipid Metabolism; Lipids/pharmacology; Lipopolysaccharides/metabolism; Lipopolysaccharides/pharmacology; Molecular Chaperones/metabolism; Periplasm/metabolism; Phospholipids/metabolism; Phospholipids/pharmacology; Porins/metabolism; Protein Binding/drug effects; Protein Folding |
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Significance
Annotations
Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
---|---|---|---|---|---|---|---|---|
involved_in |
GO:0022417: protein maturation by protein folding |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
enables |
GO:0051082: unfolded protein binding |
ECO:0000353: physical interaction evidence used in manual assertion |
UniProtKB:P02943 |
F |
Seeded From UniProt |
complete | ||
GO:0022417: protein maturation by protein folding |
ECO:0000314: |
P |
Fig. 4C shows the maturation on PhoE in Skp serum. Table 1 shows amount of PhoE folded based on incdreasing concentraions of Skp. |
complete | ||||
enables |
GO:0051082: unfolded protein binding |
ECO:0000353: physical interaction evidence used in manual assertion |
UniProtKB:P02932 |
F |
Seeded From UniProt |
complete | ||
See also
References
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