GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:9914480

From GONUTS
Jump to: navigation, search
Citation

De Cock, H, Schäfer, U, Potgeter, M, Demel, R, Müller, M and Tommassen, J (1999) Affinity of the periplasmic chaperone Skp of Escherichia coli for phospholipids, lipopolysaccharides and non-native outer membrane proteins. Role of Skp in the biogenesis of outer membrane protein. Eur. J. Biochem. 259:96-103

Abstract

The Skp protein of Escherichia coli has been proposed to be a periplasmic molecular chaperone involved in the biogenesis of outer membrane proteins. In this study, evidence is obtained that Skp exists in two different states characterized by their different sensitivity to proteases. The conversion between these states can be modulated in vitro by phospholipids, lipopolysaccharides and bivalent cations. Skp is able to associate with and insert into phospholipid membranes in vitro, indicating that it may associate with phospholipids in the inner and/or outer membrane in vivo. In addition, it interacts specifically with outer membrane proteins that are in their non-native state. We propose that Skp is required in vivo for the efficient targeting of unfolded outer membrane proteins to the membrane.

Links

PubMed

Keywords

Bacterial Outer Membrane Proteins/metabolism; DNA-Binding Proteins/metabolism; Endopeptidases/metabolism; Escherichia coli/metabolism; Escherichia coli Proteins; Lipid Metabolism; Lipids/pharmacology; Lipopolysaccharides/metabolism; Lipopolysaccharides/pharmacology; Molecular Chaperones/metabolism; Periplasm/metabolism; Phospholipids/metabolism; Phospholipids/pharmacology; Porins/metabolism; Protein Binding/drug effects; Protein Folding

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ECOLI:SKP

involved_in

GO:0022417: protein maturation by protein folding

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:SKP

enables

GO:0051082: unfolded protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P02943
UniProtKB:P0A910

F

Seeded From UniProt

complete

ECOLI:SKP

GO:0022417: protein maturation by protein folding

ECO:0000314:

P

Fig. 4C shows the maturation on PhoE in Skp serum. Table 1 shows amount of PhoE folded based on incdreasing concentraions of Skp.

complete
CACAO 2085

ECOLI:SKP

enables

GO:0051082: unfolded protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P02932

F

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.