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PMID:9852078

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Citation

Steegmaier, M, Yang, B, Yoo, JS, Huang, B, Shen, M, Yu, S, Luo, Y and Scheller, RH (1998) Three novel proteins of the syntaxin/SNAP-25 family. J. Biol. Chem. 273:34171-9

Abstract

Intracellular membrane traffic is thought to be regulated in part by soluble N-ethylmaleimide-sensitive factor-attachment protein receptors (SNAREs) through the formation of complexes between these proteins present on vesicle and target membranes. All known SNARE-mediated fusion events involve members of the syntaxin and vesicle-associated membrane protein families. The diversity of mammalian membrane compartments predicts the existence of a large number of different syntaxin and vesicle-associated membrane protein genes. To further investigate the spectrum of SNAREs and their roles in membrane trafficking we characterized three novel members of the syntaxin and SNAP-25 (synaptosome-associated protein of 25 kDa) subfamilies. The proteins are broadly expressed, suggesting a general role in vesicle trafficking, and localize to distinct membrane compartments. Syntaxin 8 co-localizes with markers of the endoplasmic reticulum. Syntaxin 17, a divergent member of the syntaxin family, partially overlaps with endoplasmic reticulum markers, and SNAP-29 is broadly localized on multiple membranes. SNAP-29 does not contain a predicted membrane anchor characteristic of other SNAREs. In vitro studies established that SNAP-29 is capable of binding to a broad range of syntaxins.

Links

PubMed

Keywords

Amino Acid Sequence; Animals; Blotting, Northern; COS Cells; Cell Line; Databases, Factual; Endoplasmic Reticulum/metabolism; Ethylmaleimide/pharmacology; Evolution, Molecular; Humans; Membrane Proteins/chemistry; Membrane Proteins/genetics; Membrane Proteins/isolation & purification; Molecular Sequence Data; Multigene Family; Nerve Tissue Proteins/chemistry; Nerve Tissue Proteins/genetics; Nerve Tissue Proteins/metabolism; Phylogeny; Qa-SNARE Proteins; Rats; Recombinant Proteins/biosynthesis; Recombinant Proteins/chemistry; Sequence Alignment; Sequence Homology, Amino Acid; Synaptosomal-Associated Protein 25; Transfection

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:SNP29

involved_in

GO:0006903: vesicle targeting

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

HUMAN:SNP29

located_in

GO:0005886: plasma membrane

ECO:0000304: author statement supported by traceable reference used in manual assertion

C

Seeded From UniProt

complete

HUMAN:STX8

located_in

GO:0005783: endoplasmic reticulum

ECO:0000304: author statement supported by traceable reference used in manual assertion

C

Seeded From UniProt

complete

RAT:STX17

located_in

GO:0005783: endoplasmic reticulum

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:STX8

part_of

GO:0005783: endoplasmic reticulum

ECO:0000304: author statement supported by traceable reference used in manual assertion

C

Seeded From UniProt

complete


See also

References

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