PMID:9819359

Citation	Boudonck, K, Dolan, L and Shaw, PJ (1998) Coiled body numbers in the Arabidopsis root epidermis are regulated by cell type, developmental stage and cell cycle parameters. J. Cell. Sci. 111 ( Pt 24):3687-94
Abstract	We have used whole mount immunofluorescence labelling with the antibody 4G3, raised against the human snRNP-specific protein U2B", and whole mount in situ hybridization with an anti-sense probe to a conserved region of U2 snRNA, in combination with confocal microscopy, to examine the organization of spliceosomal components throughout the development of the Arabidopsis thaliana root epidermis. We show that the number of coiled bodies, nuclear organelles in which splicing snRNPs and snRNAs concentrate, is developmentally regulated in the Arabidopsis root epidermis. Firstly, there is a progression from a small number of coiled bodies in the quiescent centre and initial cells, to a larger number in the cell division zone, returning to a lower number in the cell elongation and differentiation zone. Secondly, trichoblasts (root-hair forming epidermal cells) have on average 1.5 times more and often smaller coiled bodies than atrichoblasts (hairless epidermal cells). Moreover, we have shown that these differences in coiled body numbers are related to differences in cell cycle stage, cell type and developmental stage, but are not due to differences in nucleolar or general metabolic activity per se. We discuss possible explanations, including a model in which coiled bodies coalesce during interphase, for the developmental dynamics of coiled bodies.
Links	PubMed
Keywords	Arabidopsis; Autoantigens; Cell Cycle/physiology; Cell Differentiation/physiology; Cell Nucleolus/metabolism; Chromatin/chemistry; Humans; In Situ Hybridization; Plant Epidermis/chemistry; Plant Epidermis/cytology; Plant Epidermis/growth & development; Plant Roots/chemistry; Plant Roots/cytology; RNA, Small Nuclear/analysis; Ribonucleoprotein, U2 Small Nuclear/analysis; Ribonucleoprotein, U2 Small Nuclear/immunology; Ribonucleoproteins, Small Nuclear; Spliceosomes; Transcription, Genetic; snRNP Core Proteins
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
ARATH:RU2B1	located_in	GO:0015030: Cajal body	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
ARATH:RU2B2	located_in	GO:0015030: Cajal body	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete

References

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