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PMID:9784496

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Citation

Davies, RC, Calvio, C, Bratt, E, Larsson, SH, Lamond, AI and Hastie, ND (1998) WT1 interacts with the splicing factor U2AF65 in an isoform-dependent manner and can be incorporated into spliceosomes. Genes Dev. 12:3217-25

Abstract

WT1 is essential for normal kidney development, and genetic alterations are associated with Wilms' tumor, Denys Drash (DDS), and Frasier syndromes. Although generally considered a transcription factor this study has revealed that WT1 interacts with an essential splicing factor, U2AF65, and associates with the splicing machinery. WT1 is alternatively spliced and isoforms that include three amino acids, KTS, show stronger interaction with U2AF65 in vitro and better colocalization with splicing factors in vivo. Interestingly a mutation associated with DDS enhanced both -KTS WT1 binding to U2AF65 and splicing-factor colocalization. These data illustrate the functional importance of WT1 isoforms and suggest that WT1 plays a role in pre-mRNA splicing.

Links

PubMed PMC317218

Keywords

Alternative Splicing; Animals; COS Cells; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; DNA-Binding Proteins/physiology; Genes, Wilms Tumor; Humans; Male; Nuclear Proteins; Protein Isoforms/genetics; Protein Isoforms/physiology; Ribonucleoproteins/metabolism; Spliceosomes/metabolism; Transcription Factors/genetics; Transcription Factors/metabolism; Transcription Factors/physiology; Tumor Cells, Cultured; WT1 Proteins

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:WT1

located_in

GO:0016607: nuclear speck

ECO:0000304: author statement supported by traceable reference used in manual assertion

C

Seeded From UniProt

complete

MOUSE:WT1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P26369

F

Seeded From UniProt

complete

MOUSE:U2AF2

involved_in

GO:0008380: RNA splicing

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:U2AF2

located_in

GO:0016607: nuclear speck

ECO:0000304: author statement supported by traceable reference used in manual assertion

C

Seeded From UniProt

complete

MOUSE:U2AF2

enables

GO:0070742: C2H2 zinc finger domain binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P22561

F

Seeded From UniProt

complete

MOUSE:WT1

part_of

GO:0016607: nuclear speck

ECO:0000304: author statement supported by traceable reference used in manual assertion

C

Seeded From UniProt

complete

MOUSE:WT1

involved_in

GO:0008380: RNA splicing

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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