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PMID:9784496
| Citation |
Davies, RC, Calvio, C, Bratt, E, Larsson, SH, Lamond, AI and Hastie, ND (1998) WT1 interacts with the splicing factor U2AF65 in an isoform-dependent manner and can be incorporated into spliceosomes. Genes Dev. 12:3217-25 |
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| Abstract |
WT1 is essential for normal kidney development, and genetic alterations are associated with Wilms' tumor, Denys Drash (DDS), and Frasier syndromes. Although generally considered a transcription factor this study has revealed that WT1 interacts with an essential splicing factor, U2AF65, and associates with the splicing machinery. WT1 is alternatively spliced and isoforms that include three amino acids, KTS, show stronger interaction with U2AF65 in vitro and better colocalization with splicing factors in vivo. Interestingly a mutation associated with DDS enhanced both -KTS WT1 binding to U2AF65 and splicing-factor colocalization. These data illustrate the functional importance of WT1 isoforms and suggest that WT1 plays a role in pre-mRNA splicing. |
| Links | |
| Keywords |
Alternative Splicing; Animals; COS Cells; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; DNA-Binding Proteins/physiology; Genes, Wilms Tumor; Humans; Male; Nuclear Proteins; Protein Isoforms/genetics; Protein Isoforms/physiology; Ribonucleoproteins/metabolism; Spliceosomes/metabolism; Transcription Factors/genetics; Transcription Factors/metabolism; Transcription Factors/physiology; Tumor Cells, Cultured; WT1 Proteins |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
located_in |
GO:0016607: nuclear speck |
ECO:0000304: author statement supported by traceable reference used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
enables |
GO:0005515: protein binding |
ECO:0000353: physical interaction evidence used in manual assertion |
UniProtKB:P26369 |
F |
Seeded From UniProt |
complete | ||
|
MOUSE:U2AF2 |
involved_in |
GO:0008380: RNA splicing |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
MOUSE:U2AF2 |
located_in |
GO:0016607: nuclear speck |
ECO:0000304: author statement supported by traceable reference used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
MOUSE:U2AF2 |
enables |
GO:0070742: C2H2 zinc finger domain binding |
ECO:0000353: physical interaction evidence used in manual assertion |
UniProtKB:P22561 |
F |
Seeded From UniProt |
complete | |
|
part_of |
GO:0016607: nuclear speck |
ECO:0000304: author statement supported by traceable reference used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0008380: RNA splicing |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
See also
References
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