GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:9765574

From GONUTS
Jump to: navigation, search
Citation

Zientz, E, Bongaerts, J and Unden, G (1998) Fumarate regulation of gene expression in Escherichia coli by the DcuSR (dcuSR genes) two-component regulatory system. J. Bacteriol. 180:5421-5

Abstract

In Escherichia coli the genes encoding the anaerobic fumarate respiratory system are transcriptionally regulated by C4-dicarboxylates. The regulation is effected by a two-component regulatory system, DcuSR, consisting of a sensory histidine kinase (DcuS) and a response regulator (DcuR). DcuS and DcuR are encoded by the dcuSR genes (previously yjdHG) at 93.7 min on the calculated E. coli map. Inactivation of the dcuR and dcuS genes caused the loss of C4-dicarboxylate-stimulated synthesis of fumarate reductase (frdABCD genes) and of the anaerobic fumarate-succinate antiporter DcuB (dcuB gene). DcuS is predicted to contain a large periplasmic domain as the supposed site for C4-dicarboxylate sensing. Regulation by DcuR and DcuS responded to the presence of the C4-dicarboxylates fumarate, succinate, malate, aspartate, tartrate, and maleate. Since maleate is not taken up by the bacteria under these conditions, the carboxylates presumably act from without. Genes of the aerobic C4-dicarboxylate pathway encoding succinate dehydrogenase (sdhCDAB) and the aerobic succinate carrier (dctA) are only marginally or negatively regulated by the DcuSR system. The CitAB two-component regulatory system, which is highly similar to DcuSR, had no effect on C4-dicarboxylate regulation of any of the genes.

Links

PubMed PMC107591

Keywords

Antiporters/biosynthesis; Bacterial Proteins/biosynthesis; Carrier Proteins; DNA-Binding Proteins; Dicarboxylic Acid Transporters; Dicarboxylic Acids/pharmacology; Escherichia coli/drug effects; Escherichia coli/genetics; Escherichia coli Proteins; Fumarates/pharmacology; Gene Expression Regulation, Bacterial; Genes, Bacterial; Membrane Proteins/biosynthesis; Mutagenesis, Insertional; Protein Kinases/genetics; Signal Transduction/genetics; Succinate Dehydrogenase/biosynthesis; Transcription Factors

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ECOLI:DCUR

acts_upstream_of_or_within

GO:0006351: transcription, DNA-templated

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:DCUR

acts_upstream_of_or_within

GO:0045893: positive regulation of transcription, DNA-templated

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:DCUR

involved_in

GO:0006351: transcription, DNA-templated

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:DCUR

involved_in

GO:0045893: positive regulation of transcription, DNA-templated

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

See also

References

See Help:References for how to manage references in GONUTS.