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PMID:9765550

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Citation

Kliebenstein, DJ, Monde, RA and Last, RL (1998) Superoxide dismutase in Arabidopsis: an eclectic enzyme family with disparate regulation and protein localization. Plant Physiol. 118:637-50

Abstract

A number of environmental stresses can lead to enhanced production of superoxide within plant tissues, and plants are believed to rely on the enzyme superoxide dismutase (SOD) to detoxify this reactive oxygen species. We have identified seven cDNAs and genes for SOD in Arabidopsis. These consist of three CuZnSODs (CSD1, CSD2, and CSD3), three FeSODs (FSD1, FSD2, and FSD3), and one MnSOD (MSD1). The chromosomal location of these seven SOD genes has been established. To study this enzyme family, antibodies were generated against five proteins: CSD1, CSD2, CSD3, FSD1, and MSD1. Using these antisera and nondenaturing-polyacrylamide gel electrophoresis enzyme assays, we identified protein and activity for two CuZnSODs and for FeSOD and MnSOD in Arabidopsis rosette tissue. Additionally, subcellular fractionation studies revealed the presence of CSD2 and FeSOD protein within Arabidopsis chloroplasts. The seven SOD mRNAs and the four proteins identified were differentially regulated in response to various light regimes, ozone fumigation, and ultraviolet-B irradiation. To our knowledge, this is the first report of a large-scale analysis of the regulation of multiple SOD proteins in a plant species.

Links

PubMed PMC34840

Keywords

Amino Acid Sequence; Arabidopsis/enzymology; Arabidopsis/genetics; Arabidopsis/growth & development; Base Sequence; Biological Evolution; Circadian Rhythm; DNA Primers; Genes; Humans; Isoenzymes/genetics; Isoenzymes/metabolism; Light; Molecular Sequence Data; RNA, Messenger/genetics; RNA, Messenger/metabolism; Sequence Homology, Amino Acid; Superoxide Dismutase/genetics; Superoxide Dismutase/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:SODC2

located_in

GO:0009507: chloroplast

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:SODC2

involved_in

GO:0071493: cellular response to UV-B

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:SODC2

involved_in

GO:0071484: cellular response to light intensity

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:SODC2

involved_in

GO:0071457: cellular response to ozone

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:SODF1

involved_in

GO:0010193: response to ozone

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:SODF1

involved_in

GO:0009642: response to light intensity

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:SODF1

involved_in

GO:0007623: circadian rhythm

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:SODC1

involved_in

GO:0071493: cellular response to UV-B

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:SODC1

involved_in

GO:0071484: cellular response to light intensity

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:SODC1

involved_in

GO:0071457: cellular response to ozone

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:SODC1

enables

GO:0004784: superoxide dismutase activity

ECO:0000304: author statement supported by traceable reference used in manual assertion

F

Seeded From UniProt

complete

ARATH:SODC3

involved_in

GO:0071493: cellular response to UV-B

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:SODC3

involved_in

GO:0071486: cellular response to high light intensity

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:SODC3

involved_in

GO:0071484: cellular response to light intensity

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:SODC3

involved_in

GO:0071457: cellular response to ozone

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:SODC3

involved_in

GO:0006979: response to oxidative stress

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

ARATH:SODC3

enables

GO:0004784: superoxide dismutase activity

ECO:0000250: sequence similarity evidence used in manual assertion

GB:AF034832

F

Seeded From UniProt

complete

ARATH:SODC3

located_in

GO:0005777: peroxisome

ECO:0000303: author statement without traceable support used in manual assertion

C

Seeded From UniProt

complete

ARATH:SODF2

involved_in

GO:0009411: response to UV

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:SODF2

enables

GO:0004784: superoxide dismutase activity

ECO:0000250: sequence similarity evidence used in manual assertion

F

Seeded From UniProt

complete

See also

References

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