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PMID:9707581

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Citation

de la Rosa, EJ, Vega-Núñez, E, Morales, AV, Serna, J, Rubio, E and de Pablo, F (1998) Modulation of the chaperone heat shock cognate 70 by embryonic (pro)insulin correlates with prevention of apoptosis. Proc. Natl. Acad. Sci. U.S.A. 95:9950-5

Abstract

Insights have emerged concerning insulin function during development, from the finding that apoptosis during chicken embryo neurulation is prevented by prepancreatic (pro)insulin. While characterizing the molecules involved in this survival effect of insulin, we found insulin-dependent regulation of the molecular chaperone heat shock cognate 70 kDa (Hsc70), whose cloning in chicken is reported here. This chaperone, generally considered constitutively expressed, showed regulation of its mRNA and protein levels in unstressed embryos during early development. More important, Hsc70 levels were found to depend on endogenous (pro)insulin, as shown by using antisense oligodeoxynucleotides against (pro)insulin mRNA in cultured neurulating embryos. Further, in the cultured embryos, apoptosis affected mainly cells with the lowest level of Hsc70, as shown by simultaneous Hsc70 immunostaining and terminal deoxynucleotidyltransferase-mediated UTP nick end labeling. These results argue in favor of Hsc70 involvement, modulated by embryonic (pro)insulin, in the prevention of apoptosis during early development and suggest a role for a molecular chaperone in normal embryogenesis.

Links

PubMed PMC21442

Keywords

Amino Acid Sequence; Animals; Apoptosis/genetics; Apoptosis/physiology; Base Sequence; Carrier Proteins/genetics; Carrier Proteins/physiology; Chick Embryo; Cloning, Molecular; Cysteine Endopeptidases/physiology; DNA Primers/genetics; DNA, Complementary/genetics; Gene Expression Regulation, Developmental; HSC70 Heat-Shock Proteins; HSP70 Heat-Shock Proteins/genetics; Heat-Shock Proteins; Humans; Molecular Chaperones/genetics; Molecular Chaperones/physiology; Molecular Sequence Data; Polymerase Chain Reaction; Proinsulin/physiology; Sequence Homology, Amino Acid

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CHICK:A0A1D5PFJ6

involved_in

GO:0007369: gastrulation

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:A0A1D5PFJ6

involved_in

GO:0009792: embryo development ending in birth or egg hatching

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:A0A1D5PFJ6

involved_in

GO:0048568: embryonic organ development

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:F1NWP3

involved_in

GO:0007369: gastrulation

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:F1NWP3

involved_in

GO:0009792: embryo development ending in birth or egg hatching

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:F1NWP3

involved_in

GO:0048568: embryonic organ development

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:INS

involved_in

GO:0010628: positive regulation of gene expression

ECO:0000314: direct assay evidence used in manual assertion

P

  • occurs_in:(UBERON:0000922)

Seeded From UniProt

complete

CHICK:INS

involved_in

GO:0043066: negative regulation of apoptotic process

ECO:0000314: direct assay evidence used in manual assertion

P

  • occurs_in:(UBERON:0000922)

Seeded From UniProt

complete

CHICK:HSP7C

involved_in

GO:0007369: gastrulation

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:HSP7C

involved_in

GO:0048568: embryonic organ development

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:HSP7C

involved_in

GO:0009792: embryo development ending in birth or egg hatching

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:INS

involved_in

GO:0043066: negative regulation of apoptotic process

ECO:0000314: direct assay evidence used in manual assertion

P

occurs_in:(UBERON:0000922)

Seeded From UniProt

complete

CHICK:INS

involved_in

GO:0010628: positive regulation of gene expression

ECO:0000314: direct assay evidence used in manual assertion

P

occurs_in:(UBERON:0000922)

Seeded From UniProt

complete

CHICK:Q9PWN6

involved_in

GO:0043066: negative regulation of apoptotic process

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete


See also

References

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