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PMID:9651580

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Citation

Paull, TT and Gellert, M (1998) The 3' to 5' exonuclease activity of Mre 11 facilitates repair of DNA double-strand breaks. Mol. Cell 1:969-79

Abstract

MRE11 and RAD50 are known to be required for nonhomologous joining of DNA ends in vivo. We have investigated the enzymatic activities of the purified proteins and found that Mre11 by itself has 3' to 5' exonuclease activity that is increased when Mre11 is in a complex with Rad50. Mre11 also exhibits endonuclease activity, as shown by the asymmetric opening of DNA hairpin loops. In conjunction with a DNA ligase, Mre11 promotes the joining of noncomplementary ends in vitro by utilizing short homologies near the ends of the DNA fragments. Sequence identities of 1-5 base pairs are present at all of these junctions, and their diversity is consistent with the products of nonhomologous end-joining observed in vivo.

Links

PubMed

Keywords

Amino Acid Sequence; Base Sequence; DNA; DNA Damage; DNA Ligases/metabolism; DNA Repair; DNA, Complementary/chemistry; DNA, Complementary/metabolism; DNA-Binding Proteins; Endodeoxyribonucleases; Exodeoxyribonuclease V; Exodeoxyribonucleases/chemistry; Exodeoxyribonucleases/genetics; Exodeoxyribonucleases/metabolism; Fungal Proteins/chemistry; Fungal Proteins/genetics; Fungal Proteins/metabolism; HeLa Cells; Humans; Molecular Sequence Data; Nucleic Acid Conformation; Nucleic Acid Heteroduplexes/chemistry; Nucleic Acid Heteroduplexes/metabolism; Protein Conformation; Recombination, Genetic; Saccharomyces cerevisiae Proteins; Sequence Homology, Amino Acid

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:MRE11

enables

GO:0004520: endodeoxyribonuclease activity

ECO:0000304: author statement supported by traceable reference used in manual assertion

F

Seeded From UniProt

complete

HUMAN:MRE11

located_in

GO:0005654: nucleoplasm

ECO:0000304: author statement supported by traceable reference used in manual assertion

C

Seeded From UniProt

complete

HUMAN:MRE11

involved_in

GO:0006303: double-strand break repair via nonhomologous end joining

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

HUMAN:MRE11

enables

GO:0008408: 3'-5' exonuclease activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:MRE11

involved_in

GO:0006303: double-strand break repair via nonhomologous end joining

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:MRE11

enables

GO:0008408: 3'-5' exonuclease activity

ECO:0000304: author statement supported by traceable reference used in manual assertion

F

Seeded From UniProt

complete

HUMAN:MRE11

GO:0008408: 3'-5' exonuclease activity

ECO:0000314:

F

Figure 2

complete
CACAO 2030

HUMAN:MRE11

GO:0006303: double-strand break repair via nonhomologous end joining

ECO:0000314:

P

Figure 5

complete
CACAO 2032

SCHPO:RAD32

enables

GO:0000014: single-stranded DNA endodeoxyribonuclease activity

ECO:0000266: sequence orthology evidence used in manual assertion

UniProtKB:P49959

F

Seeded From UniProt

complete

SCHPO:RAD32

enables

GO:0008310: single-stranded DNA 3'-5' exodeoxyribonuclease activity

ECO:0000266: sequence orthology evidence used in manual assertion

UniProtKB:P49959

F

Seeded From UniProt

complete

SCHPO:RAD32

enables

GO:0008311: double-stranded DNA 3'-5' exodeoxyribonuclease activity

ECO:0000266: sequence orthology evidence used in manual assertion

UniProtKB:P49959

F

Seeded From UniProt

complete


See also

References

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