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PMID:9642203

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Citation

Braun, P, de Groot, A, Bitter, W and Tommassen, J (1998) Secretion of elastinolytic enzymes and their propeptides by Pseudomonas aeruginosa. J. Bacteriol. 180:3467-9

Abstract

Elastase of Pseudomonas aeruginosa is synthesized as a preproenzyme. The signal sequence is cleaved off during transport across the inner membrane and, in the periplasm, proelastase is further processed. We demonstrate that the propeptide and the mature elastase are both secreted but that the propeptide is degraded extracellularly. In addition, reduction of the extracellular proteolytic activity led to the accumulation of unprocessed forms of LasA and LasD in the extracellular medium, which shows that these enzymes are secreted in association with their propeptides. Furthermore, a hitherto undefined protein with homology to a Streptomyces griseus aminopeptidase accumulated under these conditions.

Links

PubMed PMC107305

Keywords

Bacterial Proteins; Cell Membrane/metabolism; Enzyme Precursors/biosynthesis; Enzyme Precursors/metabolism; Metalloendopeptidases/genetics; Metalloendopeptidases/metabolism; Pancreatic Elastase/biosynthesis; Pancreatic Elastase/metabolism; Periplasm/enzymology; Pseudomonas aeruginosa/enzymology; Pseudomonas aeruginosa/genetics; Serine Endopeptidases/genetics; Serine Endopeptidases/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

PSEAE:LAP

involved_in

GO:0015628: protein secretion by the type II secretion system

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

PSEAE:LAP

involved_in

GO:0043952: protein transport by the Sec complex

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

PSEAE:ELAS

involved_in

GO:0015628: protein secretion by the type II secretion system

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

PSEAE:ELAS

involved_in

GO:0043952: protein transport by the Sec complex

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

PSEAE:LASA

enables

GO:0004175: endopeptidase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

PSEAE:LASA

involved_in

GO:0015628: protein secretion by the type II secretion system

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

PSEAE:LASA

involved_in

GO:0043952: protein transport by the Sec complex

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

Notes

See also

References

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