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PMID:9642203
| Citation |
Braun, P, de Groot, A, Bitter, W and Tommassen, J (1998) Secretion of elastinolytic enzymes and their propeptides by Pseudomonas aeruginosa. J. Bacteriol. 180:3467-9 |
|---|---|
| Abstract |
Elastase of Pseudomonas aeruginosa is synthesized as a preproenzyme. The signal sequence is cleaved off during transport across the inner membrane and, in the periplasm, proelastase is further processed. We demonstrate that the propeptide and the mature elastase are both secreted but that the propeptide is degraded extracellularly. In addition, reduction of the extracellular proteolytic activity led to the accumulation of unprocessed forms of LasA and LasD in the extracellular medium, which shows that these enzymes are secreted in association with their propeptides. Furthermore, a hitherto undefined protein with homology to a Streptomyces griseus aminopeptidase accumulated under these conditions. |
| Links | |
| Keywords |
Bacterial Proteins; Cell Membrane/metabolism; Enzyme Precursors/biosynthesis; Enzyme Precursors/metabolism; Metalloendopeptidases/genetics; Metalloendopeptidases/metabolism; Pancreatic Elastase/biosynthesis; Pancreatic Elastase/metabolism; Periplasm/enzymology; Pseudomonas aeruginosa/enzymology; Pseudomonas aeruginosa/genetics; Serine Endopeptidases/genetics; Serine Endopeptidases/metabolism |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
PSEAE:LAP |
involved_in |
GO:0015628: protein secretion by the type II secretion system |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
PSEAE:LAP |
involved_in |
GO:0043952: protein transport by the Sec complex |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0015628: protein secretion by the type II secretion system |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0043952: protein transport by the Sec complex |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0004175: endopeptidase activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0015628: protein secretion by the type II secretion system |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0043952: protein transport by the Sec complex |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
Notes
See also
References
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