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PMID:9635433

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Citation

McGarry, TJ and Kirschner, MW (1998) Geminin, an inhibitor of DNA replication, is degraded during mitosis. Cell 93:1043-53

Abstract

We describe a novel 25 kDa protein, geminin, which inhibits DNA replication and is degraded during the mitotic phase of the cell cycle. Geminin has a destruction box sequence and is ubiquitinated anaphase-promoting complex (APC) in vitro. In synchronized HeLa cells, geminin is absent during G1 phase, accumulates during S, G2, and M phases, and disappears at the time of the metaphase-anaphase transition. Geminin inhibits DNA replication by preventing the incorporation of MCM complex into prereplication complex (pre-RC). We propose that geminin inhibits DNA replication during S, G2, and M phases and that geminin destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle.

Links

PubMed

Keywords

Amino Acid Sequence; Animals; Cell Cycle Proteins/chemistry; Cell Cycle Proteins/genetics; Cell Cycle Proteins/metabolism; Cell Division; Cell Nucleus/chemistry; Chromatin/metabolism; DNA Replication/physiology; DNA, Complementary/genetics; HeLa Cells; Humans; Ligases/metabolism; Mitosis/physiology; Molecular Sequence Data; Molecular Weight; Nuclear Proteins/metabolism; Recombinant Fusion Proteins; Saccharomyces cerevisiae Proteins; Sequence Homology, Amino Acid; Ubiquitin-Protein Ligase Complexes; Ubiquitin-Protein Ligases; Ubiquitins/metabolism; Xenopus laevis

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:GEMI

involved_in

GO:0008156: negative regulation of DNA replication

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:GEMI

involved_in

GO:0045786: negative regulation of cell cycle

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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