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PMID:9616126

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Citation

Tamura, M, Gu, J, Matsumoto, K, Aota, S, Parsons, R and Yamada, KM (1998) Inhibition of cell migration, spreading, and focal adhesions by tumor suppressor PTEN. Science 280:1614-7

Abstract

The tumor suppressor PTEN is a phosphatase with sequence similarity to the cytoskeletal protein tensin. Here the cellular roles of PTEN were investigated. Overexpression of PTEN inhibited cell migration, whereas antisense PTEN enhanced migration. Integrin-mediated cell spreading and the formation of focal adhesions were down-regulated by wild-type PTEN but not by PTEN with an inactive phosphatase domain. PTEN interacted with the focal adhesion kinase FAK and reduced its tyrosine phosphorylation. Overexpression of FAK partially antagonized the effects of PTEN. Thus, PTEN phosphatase may function as a tumor suppressor by negatively regulating cell interactions with the extracellular matrix.

Links

PubMed

Keywords

3T3 Cells; Animals; Cell Adhesion; Cell Adhesion Molecules/metabolism; Cell Line; Cell Movement; Cell Size; Concanavalin A; Down-Regulation; Ecdysone/pharmacology; Fibronectins; Focal Adhesion Kinase 1; Focal Adhesion Protein-Tyrosine Kinases; Genes, Tumor Suppressor; Humans; Integrins/physiology; Mice; Mutation; PTEN Phosphohydrolase; Phosphoric Monoester Hydrolases; Phosphorylation; Polylysine; Protein Tyrosine Phosphatases/genetics; Protein Tyrosine Phosphatases/metabolism; Protein Tyrosine Phosphatases/pharmacology; Protein Tyrosine Phosphatases/physiology; Protein-Tyrosine Kinases/metabolism; Recombinant Proteins/pharmacology; Signal Transduction; Transfection; Tumor Cells, Cultured; Tumor Suppressor Proteins

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:PTEN

involved_in

GO:0030336: negative regulation of cell migration

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:PTEN

involved_in

GO:0051895: negative regulation of focal adhesion assembly

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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