GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:9600082
| Citation |
Nagaki, S, Yamamoto, M, Yumoto, Y, Shirakawa, H, Yoshida, M and Teraoka, H (1998) Non-histone chromosomal proteins HMG1 and 2 enhance ligation reaction of DNA double-strand breaks. Biochem. Biophys. Res. Commun. 246:137-41 |
|---|---|
| Abstract |
DNA ligase IV in a complex with XRCC4 is responsible for DNA end-joining in repair of DNA double-strand breaks (DSB) and V(D)J recombination. We found that non-histone chromosomal high mobility group (HMG) proteins 1 and 2 enhanced the ligation of linearized pUC119 DNA with DNA ligase IV from rat liver nuclear extract. Intra-molecular and inter-molecular ligations of cohesive-ended and blunt-ended DNA were markedly stimulated by HMG1 and 2. Recombinant HMG2-domain A, B, and (A + B) polypeptides were similarly, but non-identically, effective for the stimulation of DSB ligation reaction. Ligation of single-strand breaks (nicks) was only slightly activated by the HMG proteins. The DNA end-binding Ku protein singly or in combination with the catalytic component of DNA-dependent protein kinase (DNA-PK) as the DNA-PK holoenzyme was ineffective for the ligation of linearized pUC119 DNA. Although the stimulatory effect of HMG1 and 2 on ligation of DSB in vitro was not specific to DNA ligase IV, these results suggest that HMG1 and 2 are involved in the final ligation step in DNA end-joining processes of DSB repair and V(D)J recombination. |
| Links |
PubMed Online version:10.1006/bbrc.1998.8589 |
| Keywords |
Animals; Antigens, Nuclear; Cell Nucleus/metabolism; DNA Damage; DNA Helicases; DNA Ligases/isolation & purification; DNA Ligases/metabolism; DNA Repair/drug effects; DNA Repair/physiology; DNA, Single-Stranded/metabolism; DNA-Activated Protein Kinase; DNA-Binding Proteins/metabolism; DNA-Binding Proteins/pharmacology; High Mobility Group Proteins/chemistry; High Mobility Group Proteins/metabolism; High Mobility Group Proteins/pharmacology; Humans; Liver/metabolism; Nuclear Proteins/metabolism; Nuclear Proteins/pharmacology; Protein-Serine-Threonine Kinases/metabolism; Rats; Recombinant Proteins/chemistry; Recombinant Proteins/metabolism; Recombinant Proteins/pharmacology; Swine |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
located_in |
GO:0005634: nucleus |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0006303: double-strand break repair via nonhomologous end joining |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0006303: double-strand break repair via nonhomologous end joining |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0003677: DNA binding |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0003910: DNA ligase (ATP) activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0005524: ATP binding |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005634: nucleus |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0006266: DNA ligation |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0051103: DNA ligation involved in DNA repair |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005623: cell |
ECO:0000314: direct assay evidence used in manual assertion |
C |
part_of:(UBERON:0002370) |
Seeded From UniProt |
complete | ||
| GO:1904877: positive regulation of DNA ligase activity |
ECO:0000314: |
P |
Figure 2 |
complete | ||||
|
involved_in |
GO:0051103: DNA ligation involved in DNA repair |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005623: cell |
ECO:0000314: direct assay evidence used in manual assertion |
C |
part_of:(UBERON:0002370) |
Seeded From UniProt |
complete | ||
| GO:1904877: positive regulation of DNA ligase activity |
ECO:0000314: |
P |
Figure 4 |
complete | ||||
See also
References
See Help:References for how to manage references in GONUTS.
