GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:9600082

From GONUTS
Jump to: navigation, search
Citation

Nagaki, S, Yamamoto, M, Yumoto, Y, Shirakawa, H, Yoshida, M and Teraoka, H (1998) Non-histone chromosomal proteins HMG1 and 2 enhance ligation reaction of DNA double-strand breaks. Biochem. Biophys. Res. Commun. 246:137-41

Abstract

DNA ligase IV in a complex with XRCC4 is responsible for DNA end-joining in repair of DNA double-strand breaks (DSB) and V(D)J recombination. We found that non-histone chromosomal high mobility group (HMG) proteins 1 and 2 enhanced the ligation of linearized pUC119 DNA with DNA ligase IV from rat liver nuclear extract. Intra-molecular and inter-molecular ligations of cohesive-ended and blunt-ended DNA were markedly stimulated by HMG1 and 2. Recombinant HMG2-domain A, B, and (A + B) polypeptides were similarly, but non-identically, effective for the stimulation of DSB ligation reaction. Ligation of single-strand breaks (nicks) was only slightly activated by the HMG proteins. The DNA end-binding Ku protein singly or in combination with the catalytic component of DNA-dependent protein kinase (DNA-PK) as the DNA-PK holoenzyme was ineffective for the ligation of linearized pUC119 DNA. Although the stimulatory effect of HMG1 and 2 on ligation of DSB in vitro was not specific to DNA ligase IV, these results suggest that HMG1 and 2 are involved in the final ligation step in DNA end-joining processes of DSB repair and V(D)J recombination.

Links

PubMed Online version:10.1006/bbrc.1998.8589

Keywords

Animals; Antigens, Nuclear; Cell Nucleus/metabolism; DNA Damage; DNA Helicases; DNA Ligases/isolation & purification; DNA Ligases/metabolism; DNA Repair/drug effects; DNA Repair/physiology; DNA, Single-Stranded/metabolism; DNA-Activated Protein Kinase; DNA-Binding Proteins/metabolism; DNA-Binding Proteins/pharmacology; High Mobility Group Proteins/chemistry; High Mobility Group Proteins/metabolism; High Mobility Group Proteins/pharmacology; Humans; Liver/metabolism; Nuclear Proteins/metabolism; Nuclear Proteins/pharmacology; Protein-Serine-Threonine Kinases/metabolism; Rats; Recombinant Proteins/chemistry; Recombinant Proteins/metabolism; Recombinant Proteins/pharmacology; Swine

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

RAT:D4A0U6

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

PIG:HMGB1

involved_in

GO:0006303: double-strand break repair via nonhomologous end joining

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

PIG:HMGB2

involved_in

GO:0006303: double-strand break repair via nonhomologous end joining

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

RAT:D4A0U6

enables

GO:0003677: DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

RAT:D4A0U6

enables

GO:0003910: DNA ligase (ATP) activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

RAT:D4A0U6

enables

GO:0005524: ATP binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

RAT:D4A0U6

part_of

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:D4A0U6

involved_in

GO:0006266: DNA ligation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

PIG:HMGB1

involved_in

GO:0051103: DNA ligation involved in DNA repair

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

PIG:HMGB1

part_of

GO:0005623: cell

ECO:0000314: direct assay evidence used in manual assertion

C

part_of:(UBERON:0002370)

Seeded From UniProt

complete

PIG:HMGB1

GO:1904877: positive regulation of DNA ligase activity

ECO:0000314:

P

Figure 2

complete
CACAO 11223

PIG:HMGB2

involved_in

GO:0051103: DNA ligation involved in DNA repair

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

PIG:HMGB2

part_of

GO:0005623: cell

ECO:0000314: direct assay evidence used in manual assertion

C

part_of:(UBERON:0002370)

Seeded From UniProt

complete

PIG:HMGB2

GO:1904877: positive regulation of DNA ligase activity

ECO:0000314:

P

Figure 4

complete
CACAO 11224


See also

References

See Help:References for how to manage references in GONUTS.