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PMID:9566871

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Citation

Conte, D Jr, Barber, E, Banerjee, M, Garfinkel, DJ and Curcio, MJ (1998) Posttranslational regulation of Ty1 retrotransposition by mitogen-activated protein kinase Fus3. Mol. Cell. Biol. 18:2502-13

Abstract

Ty1 retrotransposons in Saccharomyces cerevisiae are maintained in a state of transpositional dormancy. We isolated a mutation, rtt100-1, that increases the transposition of genomic Ty1 elements 18- to 56-fold but has little effect on the transposition of related Ty2 elements. rtt100-1 was shown to be a null allele of the FUS3 gene, which encodes a haploid-specific mitogen-activated protein kinase. In fus3 mutants, the levels of Ty1 RNA, protein synthesis, and proteolytic processing were not altered relative to those in FUS3 strains but steady-state levels of TyA, integrase, and reverse transcriptase proteins and Ty1 cDNA were all increased. These findings suggest that Fus3 suppresses Ty1 transposition by destabilizing viruslike particle-associated proteins. The Fus3 kinase is activated through the mating-pheromone response pathway by phosphorylation at basal levels in naive cells and at enhanced levels in pheromone-treated cells. We demonstrate that suppression of Ty1 transposition in naive cells requires basal levels of Fus3 activation. Substitution of conserved amino acids required for activation of Fus3 derepressed Ty1 transposition. Moreover, epistasis analyses revealed that components of the pheromone response pathway that act upstream of Fus3, including Ste4, Ste5, Ste7, and Ste11, are required for the posttranslational suppression of Ty1 transposition by Fus3. The regulation of Ty1 transposition by Fus3 provides a haploid-specific mechanism through which environmental signals can modulate the levels of retrotransposition.

Links

PubMed PMC110630

Keywords

Calcium-Calmodulin-Dependent Protein Kinases/genetics; Calcium-Calmodulin-Dependent Protein Kinases/metabolism; DNA, Complementary/metabolism; DNA, Fungal/metabolism; Fungal Proteins/genetics; Fungal Proteins/metabolism; Haploidy; Integrases/metabolism; Mitogen-Activated Protein Kinases; Mutation; Peptides; Pheromones; Protein Processing, Post-Translational; RNA, Fungal/metabolism; RNA-Directed DNA Polymerase/metabolism; Recombination, Genetic; Retroelements/genetics; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae Proteins; Signal Transduction

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:STE7

involved_in

GO:0010525: regulation of transposition, RNA-mediated

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:GBB

involved_in

GO:0010525: regulation of transposition, RNA-mediated

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:STE11

involved_in

GO:0010525: regulation of transposition, RNA-mediated

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:STE5

involved_in

GO:0010525: regulation of transposition, RNA-mediated

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:FUS3

involved_in

GO:0010526: negative regulation of transposition, RNA-mediated

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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