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PMID:9564032

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Citation

Sacher, M, Jiang, Y, Barrowman, J, Scarpa, A, Burston, J, Zhang, L, Schieltz, D, Yates, JR 3rd, Abeliovich, H and Ferro-Novick, S (1998) TRAPP, a highly conserved novel complex on the cis-Golgi that mediates vesicle docking and fusion. EMBO J. 17:2494-503

Abstract

We previously identified BET3 by its genetic interactions with BET1, a gene whose SNARE-like product acts in endoplasmic reticulum (ER)-to-Golgi transport. To gain insight into the function of Bet3p, we added three c-myc tags to its C-terminus and immunopurified this protein from a clarified detergent extract. Here we report that Bet3p is a member of a large complex ( approximately 800 kDa) that we call TRAPP (transport protein particle). We propose that TRAPP plays a key role in the targeting and/or fusion of ER-to-Golgi transport vesicles with their acceptor compartment. The localization of Bet3p to the cis-Golgi complex, as well as biochemical studies showing that Bet3p functions on this compartment, support this hypothesis. TRAPP contains at least nine other constituents, five of which have been identified and shown to be highly conserved novel proteins.

Links

PubMed PMC1170591 Online version:10.1093/emboj/17.9.2494

Keywords

Amino Acid Sequence; Conserved Sequence; Endoplasmic Reticulum/physiology; Epitopes; Fungal Proteins/chemistry; Fungal Proteins/isolation & purification; Fungal Proteins/metabolism; Golgi Apparatus/physiology; Green Fluorescent Proteins; Intracellular Membranes/physiology; Luminescent Proteins/metabolism; Macromolecular Substances; Membrane Fusion; Membrane Proteins/chemistry; Membrane Proteins/isolation & purification; Membrane Proteins/metabolism; Molecular Sequence Data; Mutagenesis, Site-Directed; Peptide Fragments/chemistry; Proto-Oncogene Proteins c-myc/biosynthesis; Proto-Oncogene Proteins c-myc/chemistry; Recombinant Fusion Proteins/chemistry; Recombinant Fusion Proteins/isolation & purification; Recombinant Fusion Proteins/metabolism; Saccharomyces cerevisiae/growth & development; Saccharomyces cerevisiae/physiology; Saccharomyces cerevisiae Proteins; Vesicular Transport Proteins

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:BET3

located_in

GO:0033106: cis-Golgi network membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:TRS20

involved_in

GO:0006888: endoplasmic reticulum to Golgi vesicle-mediated transport

ECO:0000316: genetic interaction evidence used in manual assertion

SGD:S000001776

P

Seeded From UniProt

complete

YEAST:TRS23

involved_in

GO:0006888: endoplasmic reticulum to Golgi vesicle-mediated transport

ECO:0000316: genetic interaction evidence used in manual assertion

SGD:S000001776

P

Seeded From UniProt

complete

YEAST:TRS33

involved_in

GO:0006888: endoplasmic reticulum to Golgi vesicle-mediated transport

ECO:0000316: genetic interaction evidence used in manual assertion

SGD:S000001776
SGD:S000004542

P

Seeded From UniProt

complete


See also

References

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