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PMID:9557739
| Citation |
Gold, MO, Yang, X, Herrmann, CH and Rice, AP (1998) PITALRE, the catalytic subunit of TAK, is required for human immunodeficiency virus Tat transactivation in vivo. J. Virol. 72:4448-53 |
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| Abstract |
The human cdc2-related kinase PITALRE is the catalytic component of TAK, the Tat-associated kinase. Previously, we have proposed that TAK is a cellular factor that mediates Tat transactivation function. Here we demonstrate that transient overexpression of PITALRE specifically squelches Tat-1 activation of both a transfected and an integrated human immunodeficiency virus type 1 (HIV-1) long terminal repeat (LTR), suggesting that PITALRE mediates Tat function as a multiprotein complex. A catalytic mutant of PITALRE, D167N, was found to be more efficient than wild-type PITALRE in squelching Tat transactivation. Neither wild-type PITALRE nor D167N was able to squelch transactivation of the human T-cell leukemia type 1 LTR by the Tax protein. Additionally, we show that artificial targeting of PITALRE to a nascent RNA element, in the absence of Tat, activated HIV-1 LTR expression. These results indicate that a PITALRE-containing complex mediates transactivation by Tat and suggest that Tat proteins function by localizing such a PITALRE-containing complex to the site of the transcribing provirus. |
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| Keywords |
Artificial Gene Fusion; Catalysis; Cyclin-Dependent Kinase 9; Gene Expression; Gene Products, tat/genetics; Genes, rev; HIV Long Terminal Repeat; HIV-1/genetics; HeLa Cells; Humans; Mutagenesis; Positive Transcriptional Elongation Factor B; Protein Kinases/genetics; Protein Kinases/metabolism; Protein-Serine-Threonine Kinases/genetics; Protein-Serine-Threonine Kinases/metabolism; Transcriptional Activation; Virus Integration; tat Gene Products, Human Immunodeficiency Virus |
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Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0001221: transcription cofactor binding |
ECO:0000314: |
F |
Figure 1b: FLAG tagged PITALRE protein bound to Tat protein complex, discerned with antibody binding |
complete | ||||
See also
References
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