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PMID:9512514

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Citation

Kadosh, D and Struhl, K (1998) Histone deacetylase activity of Rpd3 is important for transcriptional repression in vivo. Genes Dev. 12:797-805

Abstract

Eukaryotic organisms from yeast to human contain a multiprotein complex that includes Rpd3 histone deacetylase and Sin3 corepressor. The Sin3-Rpd3 complex, when recruited to promoters by specific DNA-binding proteins, can direct transcriptional repression of specific classes of target genes. It has been proposed that the histone deacetylase activity of Rpd3 is important for repression, but direct evidence is lacking. Here, we describe four Rpd3 derivatives with mutations in evolutionarily invariant histidine residues in a putative deacetylation motif. These Rpd3 mutants lack detectable histone deacetylase activity in vitro, but interact normally with Sin3 in vivo. In yeast cells, these catalytically inactive mutants are defective for transcriptional repression. They retain some residual Rpd3 function in vivo, however, suggesting that repression by the Sin3-Rpd3 complex may not be attributable exclusively to its intrinsic histone deacetylase activity. Finally, we show that a human Rpd3 homolog can interact with yeast Sin3 and repress transcription when artificially recruited to a promoter. These results suggest that the histone deacetylase activity of Rpd3 is important, but perhaps not absolutely required, for transcriptional repression in vivo.

Links

PubMed PMC316629

Keywords

Acetylation; Amino Acid Sequence; Binding Sites/genetics; Catalysis; Fungal Proteins/metabolism; Gene Expression Regulation, Fungal; Histone Deacetylases/genetics; Histone Deacetylases/metabolism; Molecular Sequence Data; Mutation/genetics; Mutation/physiology; Saccharomyces cerevisiae/enzymology; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae Proteins; Sequence Homology, Amino Acid; Transcription Factors/genetics; Transcription Factors/metabolism; Transcription, Genetic/genetics; Transcription, Genetic/physiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:SIN3

located_in

GO:0005634: nucleus

ECO:0005547: biological system reconstruction evidence based on inference from background scientific knowledge used in manual assertion

C

Seeded From UniProt

complete

YEAST:SIN3

involved_in

GO:0000122: negative regulation of transcription by RNA polymerase II

ECO:0005547: biological system reconstruction evidence based on inference from background scientific knowledge used in manual assertion

P

Seeded From UniProt

complete

YEAST:RPD3

located_in

GO:0005634: nucleus

ECO:0005547: biological system reconstruction evidence based on inference from background scientific knowledge used in manual assertion

C

Seeded From UniProt

complete

YEAST:RPD3

involved_in

GO:0000122: negative regulation of transcription by RNA polymerase II

ECO:0005547: biological system reconstruction evidence based on inference from background scientific knowledge used in manual assertion

P

Seeded From UniProt

complete

YEAST:UME1

located_in

GO:0005634: nucleus

ECO:0005547: biological system reconstruction evidence based on inference from background scientific knowledge used in manual assertion

C

Seeded From UniProt

complete

YEAST:UME1

involved_in

GO:0000122: negative regulation of transcription by RNA polymerase II

ECO:0005547: biological system reconstruction evidence based on inference from background scientific knowledge used in manual assertion

P

Seeded From UniProt

complete

YEAST:RCO1

located_in

GO:0005634: nucleus

ECO:0005547: biological system reconstruction evidence based on inference from background scientific knowledge used in manual assertion

C

Seeded From UniProt

complete

YEAST:RCO1

involved_in

GO:0000122: negative regulation of transcription by RNA polymerase II

ECO:0005547: biological system reconstruction evidence based on inference from background scientific knowledge used in manual assertion

P

Seeded From UniProt

complete

YEAST:EAF3

located_in

GO:0005634: nucleus

ECO:0005547: biological system reconstruction evidence based on inference from background scientific knowledge used in manual assertion

C

Seeded From UniProt

complete

YEAST:EAF3

involved_in

GO:0000122: negative regulation of transcription by RNA polymerase II

ECO:0005547: biological system reconstruction evidence based on inference from background scientific knowledge used in manual assertion

P

Seeded From UniProt

complete

YEAST:RPD3

enables

GO:0004407: histone deacetylase activity

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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