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PMID:9501024

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Citation

Biben, C, Stanley, E, Fabri, L, Kotecha, S, Rhinn, M, Drinkwater, C, Lah, M, Wang, CC, Nash, A, Hilton, D, Ang, SL, Mohun, T and Harvey, RP (1998) Murine cerberus homologue mCer-1: a candidate anterior patterning molecule. Dev. Biol. 194:135-51

Abstract

Xenopus cerberus (Xcer) is a cytokine expressed in anterior mesendoderm overlapping and surrounding Spemann's gastrula organiser. When misexpressed in blastomeres, Xcer can induce ectopic heads with well-defined brain, cement gland, olfactory placodes, cyclopic eye, and occasionally liver and heart. We report here the identification of mCer-1, a murine gene related to cerberus. Both mCer-1 and Xcer appear to belong to the cystine knot superfamily, which includes TGF beta s and BMPs. In Xenopus animal cap assays, mCer-1 and Xcer induced cement glands and markers of anterior neural tissue and endoderm, characteristic of BMP inhibition. Furthermore, both antagonised the ventrolateral mesoderm-inducing activity of coexpressed BMP4. In mouse embryos, mCer-1 was expressed at early gastrulation in a stripe of primitive endoderm along the future anterior side of the egg cylinder, a region essential for anterior patterning. A second phase of expression was detected in anterior embryonic mesendoderm, and by late-streak stages most of the anterior half of the embryo was positive, except for the node and cardiac progenitors. Expression was later seen in the cranial portion of the two most-recently formed somites and in two stripes within presomitic mesoderm. In embryos lacking Otx2, a homeogene with a demonstrated role in anterior patterning, mCer-1 was still expressed in an anterior zone, although often abnormally. The data suggest that mCer-1 shares structural, functional, and expression characteristics with Xcer and may participate in patterning the anterior of the embryo and nascent somite region, in part, through a BMP-inhibitory mechanism.

Links

PubMed Online version:10.1006/dbio.1997.8812

Keywords

Amino Acid Sequence; Animals; Body Patterning/genetics; CHO Cells; Cricetinae; Databases, Factual; Dimerization; Embryonic Induction; Endoderm/metabolism; Gene Expression Regulation, Developmental; Gene Library; Homeodomain Proteins/genetics; Homeodomain Proteins/metabolism; Intercellular Signaling Peptides and Proteins; Mice; Molecular Sequence Data; Nerve Tissue Proteins/genetics; Nerve Tissue Proteins/metabolism; Otx Transcription Factors; Proteins/genetics; Proteins/physiology; Sequence Alignment; Trans-Activators/genetics; Trans-Activators/metabolism; Xenopus Proteins

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:CER1

acts_upstream_of_or_within

GO:0030514: negative regulation of BMP signaling pathway

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:CER1

acts_upstream_of_or_within

GO:0009952: anterior/posterior pattern specification

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:OTX2

acts_upstream_of_or_within

GO:0009952: anterior/posterior pattern specification

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:1857502

P

Seeded From UniProt

complete

MOUSE:BMP4

acts_upstream_of_or_within

GO:0045944: positive regulation of transcription by RNA polymerase II

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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