GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:9497389

From GONUTS
Jump to: navigation, search
Citation

Dong, F, Feldmesser, M, Casadevall, A and Rubin, CS (1998) Molecular characterization of a cDNA that encodes six isoforms of a novel murine A kinase anchor protein. J. Biol. Chem. 273:6533-41

Abstract

We have cloned cDNA that encodes six novel A kinase anchor proteins (collectively named AKAP-KL). AKAP-KL diversity is generated by alternative mRNA splicing and utilization of two translation initiation codons. AKAP-KL polypeptides are evident in lung, kidney, and cerebellum, but are absent from many tissues. Different isoforms predominate in different tissues. Thus, AKAP-KL expression is differentially regulated in vivo. All AKAP-KL isoforms contain a 20-residue domain that avidly binds (Kd approximately 10 nM) regulatory subunits (RII) of protein kinase AII and is highly homologous with the RII tethering site in neuronal AKAP75. The distribution of AKAP-KL is strikingly asymmetric (polarized) in situ. Anchor protein accumulates near the inner, apical surface of highly polarized epithelium in tubules of nephrons. Both RII and AKAP-KL are enriched at an intracellular site that lies just below the plasma membrane of alveolar epithelial cells in lung. AKAP-KL interacts with and modulates the structure of the actin cytoskeleton in transfected cells. We also demonstrate that the tethering domain of AKAP-KL avidly ligates RII subunits in intact cells. AKAP-KL may be involved in (a) establishing polarity in signaling systems and (b) physically and functionally integrating PKAII isoforms with downstream effectors to capture, amplify, and precisely focus diffuse, trans-cellular signals carried by cAMP.

Links

PubMed

Keywords

A Kinase Anchor Proteins; Adaptor Proteins, Signal Transducing; Amino Acid Sequence; Animals; Base Sequence; Binding Sites; Carrier Proteins/genetics; Carrier Proteins/metabolism; Cell Compartmentation; Cell Polarity/physiology; Cyclic AMP-Dependent Protein Kinases/genetics; Cyclic AMP-Dependent Protein Kinases/metabolism; Cytoskeleton/metabolism; DNA, Complementary/genetics; Epithelial Cells/enzymology; Isoenzymes/genetics; Isoenzymes/metabolism; Kidney/enzymology; Lung/enzymology; Membrane Proteins/genetics; Membrane Proteins/metabolism; Mice; Molecular Sequence Data; Protein Binding; Protein Biosynthesis; Sequence Homology, Amino Acid; Signal Transduction; Tissue Distribution

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:A0A5F8MPZ6

acts_upstream_of_or_within

GO:0008104: protein localization

ECO:0000353: physical interaction evidence used in manual assertion

PR:P12367

P

Seeded From UniProt

complete

MOUSE:A0A5F8MPZ6

acts_upstream_of_or_within

GO:0007178: transmembrane receptor protein serine/threonine kinase signaling pathway

ECO:0000353: physical interaction evidence used in manual assertion

PR:P12367

P

Seeded From UniProt

complete

MOUSE:A0A5F8MPZ6

acts_upstream_of_or_within

GO:0007015: actin filament organization

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:A0A5F8MPZ6

enables

GO:0051018: protein kinase A binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:P12367

F

Seeded From UniProt

complete

MOUSE:A2API8

acts_upstream_of_or_within

GO:0008104: protein localization

ECO:0000353: physical interaction evidence used in manual assertion

PR:P12367

P

Seeded From UniProt

complete

MOUSE:A2API8

acts_upstream_of_or_within

GO:0007178: transmembrane receptor protein serine/threonine kinase signaling pathway

ECO:0000353: physical interaction evidence used in manual assertion

PR:P12367

P

Seeded From UniProt

complete

MOUSE:A2API8

acts_upstream_of_or_within

GO:0007015: actin filament organization

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:A2API8

enables

GO:0051018: protein kinase A binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:P12367

F

Seeded From UniProt

complete

MOUSE:A2API9

acts_upstream_of_or_within

GO:0008104: protein localization

ECO:0000353: physical interaction evidence used in manual assertion

PR:P12367

P

Seeded From UniProt

complete

MOUSE:A2API9

acts_upstream_of_or_within

GO:0007178: transmembrane receptor protein serine/threonine kinase signaling pathway

ECO:0000353: physical interaction evidence used in manual assertion

PR:P12367

P

Seeded From UniProt

complete

MOUSE:A2API9

acts_upstream_of_or_within

GO:0007015: actin filament organization

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:A2API9

enables

GO:0051018: protein kinase A binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:P12367

F

Seeded From UniProt

complete

MOUSE:A2APJ1

acts_upstream_of_or_within

GO:0008104: protein localization

ECO:0000353: physical interaction evidence used in manual assertion

PR:P12367

P

Seeded From UniProt

complete

MOUSE:A2APJ1

acts_upstream_of_or_within

GO:0007178: transmembrane receptor protein serine/threonine kinase signaling pathway

ECO:0000353: physical interaction evidence used in manual assertion

PR:P12367

P

Seeded From UniProt

complete

MOUSE:A2APJ1

acts_upstream_of_or_within

GO:0007015: actin filament organization

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:A2APJ1

enables

GO:0051018: protein kinase A binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:P12367

F

Seeded From UniProt

complete

MOUSE:F7AA26

acts_upstream_of_or_within

GO:0008104: protein localization

ECO:0000353: physical interaction evidence used in manual assertion

PR:P12367

P

Seeded From UniProt

complete

MOUSE:F7AA26

acts_upstream_of_or_within

GO:0007178: transmembrane receptor protein serine/threonine kinase signaling pathway

ECO:0000353: physical interaction evidence used in manual assertion

PR:P12367

P

Seeded From UniProt

complete

MOUSE:F7AA26

acts_upstream_of_or_within

GO:0007015: actin filament organization

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:F7AA26

enables

GO:0051018: protein kinase A binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:P12367

F

Seeded From UniProt

complete

MOUSE:G3X9P1

acts_upstream_of_or_within

GO:0008104: protein localization

ECO:0000353: physical interaction evidence used in manual assertion

PR:P12367

P

Seeded From UniProt

complete

MOUSE:G3X9P1

acts_upstream_of_or_within

GO:0007178: transmembrane receptor protein serine/threonine kinase signaling pathway

ECO:0000353: physical interaction evidence used in manual assertion

PR:P12367

P

Seeded From UniProt

complete

MOUSE:G3X9P1

acts_upstream_of_or_within

GO:0007015: actin filament organization

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:G3X9P1

enables

GO:0051018: protein kinase A binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:P12367

F

Seeded From UniProt

complete

MOUSE:Q3UZT2

acts_upstream_of_or_within

GO:0008104: protein localization

ECO:0000353: physical interaction evidence used in manual assertion

PR:P12367

P

Seeded From UniProt

complete

MOUSE:Q3UZT2

acts_upstream_of_or_within

GO:0007178: transmembrane receptor protein serine/threonine kinase signaling pathway

ECO:0000353: physical interaction evidence used in manual assertion

PR:P12367

P

Seeded From UniProt

complete

MOUSE:Q3UZT2

acts_upstream_of_or_within

GO:0007015: actin filament organization

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:Q3UZT2

enables

GO:0051018: protein kinase A binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:P12367

F

Seeded From UniProt

complete

MOUSE:Q69ZV5

acts_upstream_of_or_within

GO:0008104: protein localization

ECO:0000353: physical interaction evidence used in manual assertion

PR:P12367

P

Seeded From UniProt

complete

MOUSE:Q69ZV5

acts_upstream_of_or_within

GO:0007178: transmembrane receptor protein serine/threonine kinase signaling pathway

ECO:0000353: physical interaction evidence used in manual assertion

PR:P12367

P

Seeded From UniProt

complete

MOUSE:Q69ZV5

acts_upstream_of_or_within

GO:0007015: actin filament organization

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:Q69ZV5

enables

GO:0051018: protein kinase A binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:P12367

F

Seeded From UniProt

complete

MOUSE:Q8C9L6

acts_upstream_of_or_within

GO:0008104: protein localization

ECO:0000353: physical interaction evidence used in manual assertion

PR:P12367

P

Seeded From UniProt

complete

MOUSE:Q8C9L6

acts_upstream_of_or_within

GO:0007178: transmembrane receptor protein serine/threonine kinase signaling pathway

ECO:0000353: physical interaction evidence used in manual assertion

PR:P12367

P

Seeded From UniProt

complete

MOUSE:Q8C9L6

acts_upstream_of_or_within

GO:0007015: actin filament organization

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:Q8C9L6

enables

GO:0051018: protein kinase A binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:P12367

F

Seeded From UniProt

complete

MOUSE:Q99L95

acts_upstream_of_or_within

GO:0008104: protein localization

ECO:0000353: physical interaction evidence used in manual assertion

PR:P12367

P

Seeded From UniProt

complete

MOUSE:Q99L95

acts_upstream_of_or_within

GO:0007178: transmembrane receptor protein serine/threonine kinase signaling pathway

ECO:0000353: physical interaction evidence used in manual assertion

PR:P12367

P

Seeded From UniProt

complete

MOUSE:Q99L95

acts_upstream_of_or_within

GO:0007015: actin filament organization

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:Q99L95

enables

GO:0051018: protein kinase A binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:P12367

F

Seeded From UniProt

complete

MOUSE:Q9Z107

acts_upstream_of_or_within

GO:0008104: protein localization

ECO:0000353: physical interaction evidence used in manual assertion

PR:P12367

P

Seeded From UniProt

complete

MOUSE:Q9Z107

acts_upstream_of_or_within

GO:0007178: transmembrane receptor protein serine/threonine kinase signaling pathway

ECO:0000353: physical interaction evidence used in manual assertion

PR:P12367

P

Seeded From UniProt

complete

MOUSE:Q9Z107

acts_upstream_of_or_within

GO:0007015: actin filament organization

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:Q9Z107

enables

GO:0051018: protein kinase A binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:P12367

F

Seeded From UniProt

complete

See also

References

See Help:References for how to manage references in GONUTS.