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PMID:9495756

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Citation

Falk-Krzesinski, HJ and Wolfe, AJ (1998) Genetic analysis of the nuo locus, which encodes the proton-translocating NADH dehydrogenase in Escherichia coli. J. Bacteriol. 180:1174-84

Abstract

Complex I (EC 1.6.99.3) of the bacterium Escherichia coli is considered to be the minimal form of the type I NADH dehydrogenase, the first enzyme complex in the respiratory chain. Because of its small size and relative simplicity, the E. coli enzyme has become a model used to identify and characterize the mechanism(s) by which cells regulate the synthesis and assembly of this large respiratory complex. To begin dissecting the processes by which E. coli cells regulate the expression of nuo and the assembly of complex I, we undertook a genetic analysis of the nuo locus, which encodes the 14 Nuo subunits comprising E. coli complex I. Here we present the results of studies, performed on an isogenic collection of nuo mutants, that focus on the physiological, biochemical, and molecular consequences caused by the lack of or defects in several Nuo subunits. In particular, we present evidence that NuoG, a peripheral subunit, is essential for complex I function and that it plays a role in the regulation of nuo expression and/or the assembly of complex I.

Links

PubMed PMC107005

Keywords

Electron Spin Resonance Spectroscopy; Electron Transport Complex I; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/physiology; Gene Expression Regulation, Bacterial; Genes, Bacterial; Genetic Complementation Test; Mutation; NAD/metabolism; NADH Dehydrogenase/chemistry; NADH Dehydrogenase/genetics; NADH Dehydrogenase/metabolism; NADH, NADPH Oxidoreductases/chemistry; NADH, NADPH Oxidoreductases/genetics; NADH, NADPH Oxidoreductases/metabolism; Phenotype; Promoter Regions, Genetic; Protein Biosynthesis; Protons

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ECOLI:NUOB

acts_upstream_of_or_within

GO:0009060: aerobic respiration

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:NUOH

acts_upstream_of_or_within

GO:0009060: aerobic respiration

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:NUOI

acts_upstream_of_or_within

GO:0009060: aerobic respiration

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:NUOM

acts_upstream_of_or_within

GO:0009060: aerobic respiration

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:NUON

acts_upstream_of_or_within

GO:0009060: aerobic respiration

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:NUOF

acts_upstream_of_or_within

GO:0009060: aerobic respiration

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:NUOG

acts_upstream_of_or_within

GO:0009060: aerobic respiration

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:NUOM

involved_in

GO:0009060: aerobic respiration

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:NUOB

enables

GO:0008137: NADH dehydrogenase (ubiquinone) activity

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete

ECOLI:NUOB

involved_in

GO:0009060: aerobic respiration

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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