PMID:9495756

Citation	Falk-Krzesinski, HJ and Wolfe, AJ (1998) Genetic analysis of the nuo locus, which encodes the proton-translocating NADH dehydrogenase in Escherichia coli. J. Bacteriol. 180:1174-84
Abstract	Complex I (EC 1.6.99.3) of the bacterium Escherichia coli is considered to be the minimal form of the type I NADH dehydrogenase, the first enzyme complex in the respiratory chain. Because of its small size and relative simplicity, the E. coli enzyme has become a model used to identify and characterize the mechanism(s) by which cells regulate the synthesis and assembly of this large respiratory complex. To begin dissecting the processes by which E. coli cells regulate the expression of nuo and the assembly of complex I, we undertook a genetic analysis of the nuo locus, which encodes the 14 Nuo subunits comprising E. coli complex I. Here we present the results of studies, performed on an isogenic collection of nuo mutants, that focus on the physiological, biochemical, and molecular consequences caused by the lack of or defects in several Nuo subunits. In particular, we present evidence that NuoG, a peripheral subunit, is essential for complex I function and that it plays a role in the regulation of nuo expression and/or the assembly of complex I.
Links	PubMed PMC107005
Keywords	Electron Spin Resonance Spectroscopy; Electron Transport Complex I; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/physiology; Gene Expression Regulation, Bacterial; Genes, Bacterial; Genetic Complementation Test; Mutation; NAD/metabolism; NADH Dehydrogenase/chemistry; NADH Dehydrogenase/genetics; NADH Dehydrogenase/metabolism; NADH, NADPH Oxidoreductases/chemistry; NADH, NADPH Oxidoreductases/genetics; NADH, NADPH Oxidoreductases/metabolism; Phenotype; Promoter Regions, Genetic; Protein Biosynthesis; Protons
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
ECOLI:NUOB	acts_upstream_of_or_within	GO:0009060: aerobic respiration	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
ECOLI:NUOH	acts_upstream_of_or_within	GO:0009060: aerobic respiration	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
ECOLI:NUOI	acts_upstream_of_or_within	GO:0009060: aerobic respiration	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
ECOLI:NUOM	acts_upstream_of_or_within	GO:0009060: aerobic respiration	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
ECOLI:NUON	acts_upstream_of_or_within	GO:0009060: aerobic respiration	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
ECOLI:NUOF	acts_upstream_of_or_within	GO:0009060: aerobic respiration	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
ECOLI:NUOG	acts_upstream_of_or_within	GO:0009060: aerobic respiration	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
ECOLI:NUOM	involved_in	GO:0009060: aerobic respiration	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
ECOLI:NUOB	enables	GO:0008137: NADH dehydrogenase (ubiquinone) activity	ECO:0000315: mutant phenotype evidence used in manual assertion		F		Seeded From UniProt	complete
ECOLI:NUOB	involved_in	GO:0009060: aerobic respiration	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

References

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