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PMID:9469814

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Citation

Zachariae, W, Shevchenko, A, Andrews, PD, Ciosk, R, Galova, M, Stark, MJ, Mann, M and Nasmyth, K (1998) Mass spectrometric analysis of the anaphase-promoting complex from yeast: identification of a subunit related to cullins. Science 279:1216-9

Abstract

Entry into anaphase and exit from mitosis depend on a ubiquitin-protein ligase complex called the anaphase-promoting complex (APC) or cyclosome. At least 12 different subunits were detected in the purified particle from budding yeast, including the previously identified proteins Apc1p, Cdc16p, Cdc23p, Cdc26p, and Cdc27p. Five additional subunits purified in low nanogram amounts were identified by tandem mass spectrometric sequencing. Apc2p, Apc5p, and the RING-finger protein Apc11p are conserved from yeast to humans. Apc2p is similar to the cullin Cdc53p, which is a subunit of the ubiquitin-protein ligase complex SCFCdc4 required for the initiation of DNA replication.

Links

PubMed

Keywords

Amino Acid Sequence; Anaphase; Animals; Cell Cycle Proteins/chemistry; Cell Cycle Proteins/metabolism; Cullin Proteins; Cyclins/metabolism; DNA Replication; Fungal Proteins/chemistry; Fungal Proteins/genetics; Fungal Proteins/isolation & purification; Genes, Fungal; Humans; Ligases/chemistry; Ligases/genetics; Ligases/isolation & purification; Mass Spectrometry; Mitotic Spindle Apparatus/metabolism; Molecular Sequence Data; Saccharomyces cerevisiae/chemistry; Saccharomyces cerevisiae/cytology; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae Proteins; Sequence Alignment; Ubiquitin-Protein Ligase Complexes; Ubiquitin-Protein Ligases; Ubiquitins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:CDC16

part_of

GO:0005680: anaphase-promoting complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:CDC16

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P53068

F

Seeded From UniProt

complete

YEAST:CDC26

part_of

GO:0005680: anaphase-promoting complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:CDC23

part_of

GO:0005680: anaphase-promoting complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:CDC23

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q12379

F

Seeded From UniProt

complete

YEAST:CDC27

part_of

GO:0005680: anaphase-promoting complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:CDC27

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P53068

F

Seeded From UniProt

complete

YEAST:APC10

part_of

GO:0005680: anaphase-promoting complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:APC10

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P09798

F

Seeded From UniProt

complete

YEAST:APC10

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P38042

F

Seeded From UniProt

complete

YEAST:APC10

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P53886

F

Seeded From UniProt

complete

YEAST:APC1

part_of

GO:0005680: anaphase-promoting complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:APC1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P53068

F

Seeded From UniProt

complete

YEAST:APC4

part_of

GO:0005680: anaphase-promoting complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:APC4

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q08683

F

Seeded From UniProt

complete

YEAST:APC5

part_of

GO:0005680: anaphase-promoting complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:APC5

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q04601

F

Seeded From UniProt

complete

YEAST:APC9

part_of

GO:0005680: anaphase-promoting complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:APC11

part_of

GO:0005680: anaphase-promoting complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:SWM1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P16522

F

Seeded From UniProt

complete

YEAST:APC2

part_of

GO:0005680: anaphase-promoting complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

See also

References

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