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PMID:9430655

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Citation

Murthy, A, Gonzalez-Agosti, C, Cordero, E, Pinney, D, Candia, C, Solomon, F, Gusella, J and Ramesh, V (1998) NHE-RF, a regulatory cofactor for Na(+)-H+ exchange, is a common interactor for merlin and ERM (MERM) proteins. J. Biol. Chem. 273:1273-6

Abstract

We have identified the human homologue of a regulatory cofactor of Na(+)-H+ exchanger (NHE-RF) as a novel interactor for merlin, the neurofibromatosis 2 tumor suppressor protein. NHE-RF mediates protein kinase A regulation of Na(+)-H+ exchanger NHE3 to which it is thought to bind via one of its two PDZ domains. The carboxyl-terminal region of NHE-RF, downstream of the PDZ domains, interacts with the amino-terminal protein 4.1 domain-containing segment of merlin in yeast two-hybrid assays. This interaction also occurs in affinity binding assays with full-length NHE-RF expressed in COS-7 cells. NHE-RF binds to the related ERM proteins, moesin and radixin. We have localized human NHE-RF to actin-rich structures such as membrane ruffles, microvilli, and filopodia in HeLa and COS-7 cells, where it co-localizes with merlin and moesin. These findings suggest that hNHE-RF and its binding partners may participate in a larger complex (one component of which might be a Na(+)-H+ exchanger) that could be crucial for the actin filament assembly activated by the ERM proteins and for the tumor suppressor function of merlin.

Links

PubMed

Keywords

Amino Acid Sequence; Animals; COS Cells; Cloning, Molecular; Cyclic AMP-Dependent Protein Kinases/metabolism; DNA-Binding Proteins/metabolism; Genes, Neurofibromatosis 2; HeLa Cells; Humans; Immunoenzyme Techniques; Membrane Proteins/metabolism; Molecular Sequence Data; Neoplasm Proteins/metabolism; Neurofibromin 2; Phosphoproteins/genetics; Phosphoproteins/metabolism; Sodium-Hydrogen Antiporter/metabolism; Transcription Factors/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:NHRF1

located_in

GO:0015629: actin cytoskeleton

ECO:0000304: author statement supported by traceable reference used in manual assertion

C

Seeded From UniProt

complete

HUMAN:NHRF1

involved_in

GO:0065003: protein-containing complex assembly

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

HUMAN:NHRF1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P26038

F

Seeded From UniProt

complete

HUMAN:NHRF1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P35240-1

F

Seeded From UniProt

complete

HUMAN:MOES

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:O14745

F

Seeded From UniProt

complete

CANLF:E2RJV1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:O14745

F

Seeded From UniProt

complete


See also

References

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