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PMID:9418043

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Citation

Van Oosten, JJ, Gerbaud, A, Huijser, C, Dijkwel, PP, Chua, NH and Smeekens, SC (1997) An Arabidopsis mutant showing reduced feedback inhibition of photosynthesis. Plant J. 12:1011-20

Abstract

Many plant genes are responsive to sugars but the mechanisms used by plants to sense sugars are unknown. A genetic approach has been used in Arabidopsis to identify genes involved in perception and transduction of sugar signals. For this purpose, an in vivo reporter system was established consisting of the light- and sugar-regulated plastocyanin promoter, fused to the luciferase coding sequence (PC-LUC construct). At the seedling stage, expression of the PC-LUC gene is repressed by sucrose, and a number of sucrose-uncoupled (sun) mutants were selected in which sucrose is unable to repress the activity of the PC promoter. Three mutants have been characterized in more detail. The sugar analog 2-deoxy-D-glucose (2DG) was used to repress whole plant photosynthesis, PC-LUC gene expression and total ribulose-1,5-bisphosphate activity. It was found that the sun6 mutation makes plants unresponsive to these 2DG-induced effects. Moreover, unlike wild-type plants, sun6 mutants are insensitive to elevated levels of glucose in the growth medium. These findings suggest that the SUN6 gene is active in a hexose-activated signal transduction pathway.

Links

PubMed

Keywords

Arabidopsis/genetics; Arabidopsis/physiology; Deoxyglucose/pharmacology; Enzyme Repression; Feedback; Gene Expression Regulation, Plant/drug effects; Genetic Linkage; Luciferases/biosynthesis; Mutagenesis; Photosynthesis/drug effects; Photosynthesis/genetics; Photosynthesis/physiology; Plants, Genetically Modified; Plastocyanin/biosynthesis; Plastocyanin/genetics; Promoter Regions, Genetic; Recombinant Fusion Proteins/biosynthesis; Ribulose-Bisphosphate Carboxylase/biosynthesis; Signal Transduction; Sucrose/pharmacology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:ABI4

acts_upstream_of_or_within

GO:0009744: response to sucrose

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

See also

References

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