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PMID:9398642

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Citation

Shinozaki, K, Maruyama, K, Kume, H, Kuzume, H and Obata, K (1997) A novel brain gene, norbin, induced by treatment of tetraethylammonium in rat hippocampal slice and accompanied with neurite-outgrowth in neuro 2a cells. Biochem. Biophys. Res. Commun. 240:766-71

Abstract

Tetraethylammonium (TEA) induces long-term potentiation (LTP)-like synaptic enhancement in rat hippocampal slices. To find the genes related to this phenomenon, subtraction screening was performed between the mRNA of TEA-treated slices and that of untreated whole brain. One of the clones induced by the TEA treatment, named as norbin, was expressed only in neural tissues. The predicted protein sequence of norbin consisted of 729 amino acids, and no homologies in the sequence were found with known genes or proteins. Overexpression of norbin in cultured Neuro 2a cells by cDNA transfection induced neurite-outgrowth. Since in the course of neural plasticity the formation of new synapses should occur, the neurite-outgrowth-related protein, norbin, might play an important role in neural plasticity.

Links

PubMed Online version:10.1006/bbrc.1997.7660

Keywords

Amino Acid Sequence; Animals; COS Cells; Cell Line; Cloning, Molecular; Gene Expression Regulation; Hippocampus/cytology; Hippocampus/drug effects; Hippocampus/metabolism; Immunoblotting; In Situ Hybridization; Isopropyl Thiogalactoside/pharmacology; Long-Term Potentiation; Microscopy, Phase-Contrast; Molecular Sequence Data; Neurites/drug effects; Neurites/physiology; Neurons/metabolism; Neuropeptides/chemistry; Neuropeptides/genetics; RNA, Messenger/analysis; RNA, Messenger/genetics; Rats; Sequence Analysis, DNA; Tetraethylammonium/pharmacology; Transfection

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

RAT:NCDN

involved_in

GO:0048168: regulation of neuronal synaptic plasticity

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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