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PMID:9398211

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Citation

Gavish, H, Bab, I, Tartakovsky, A, Chorev, M, Mansur, N, Greenberg, Z, Namdar-Attar, M and Muhlrad, A (1997) Human alpha 2-macroglobulin is an osteogenic growth peptide-binding protein. Biochemistry 36:14883-8

Abstract

The osteogenic growth peptide (OGP) is a 14mer mitogen of osteoblastic and fibroblastic cells. Physiologically, OGP is present in high abundance in human and other mammalian sera. Most of the serum OGP is complexed noncovalently to heat sensitive, high molecular weight OGP-binding proteins (OGPBPs). Changes in serum OGP levels that follow bone marrow ablation and the low doses of exogenous OGP required for the stimulation of bone formation suggest a regulatory role for the OGPBPs. In the present work, the OGP binding activity was monitored by competitive binding to [3-125I(Tyr10)]-sOGP and the corresponding complexes were demonstrated on nondenaturing cathodic polyacrylamide gel electrophoresis. We show that OGP binds to both native and activated human plasma alpha 2-macroglobulin (alpha 2M). alpha 2M was also immunoidentified in reduced and nonreduced SDS-polyacrylamide gel electrophoresis of OGP-affinity purified plasma-derived proteins. Immunoreactive OGP was detected in commercial preparations of both forms of alpha 2M; OGP was purified to homogeneity from the commercial preparation of activated alpha 2M. In MC3T3 E1 cells, native alpha 2M, at concentrations < 50 ng/mL, had a substantially increased mitogenic effect in the presence of synthetic, native-like, OGP (sOGP). Similar amounts of activated alpha 2M inhibited the sOGP proliferative effect. These results suggest that the native alpha 2M enhances the immediate availability of OGP to its target cells. Activated alpha 2M may participate in the removal of OGP from the system.

Links

PubMed Online version:10.1021/bi971670t

Keywords

Growth Substances/metabolism; Growth Substances/pharmacology; Histones; Humans; Intercellular Signaling Peptides and Proteins; Mitogens; Osteoblasts/drug effects; Osteogenesis; Peptides/metabolism; Peptides/pharmacology; Protein Binding; alpha-Macroglobulins/metabolism; alpha-Macroglobulins/pharmacology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:A2MG

enables

GO:0019838: growth factor binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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