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PMID:9384574

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Citation

Matsuyama, Si, Yokota, N and Tokuda, H (1997) A novel outer membrane lipoprotein, LolB (HemM), involved in the LolA (p20)-dependent localization of lipoproteins to the outer membrane of Escherichia coli. EMBO J. 16:6947-55

Abstract

The Escherichia coli major outer membrane lipoprotein (Lpp) is released from the inner membrane into the periplasm as a complex with a carrier protein, LolA (p20), and is then specifically incorporated into the outer membrane. An outer membrane protein playing a critical role in Lpp incorporation was identified, and partial amino acid sequences of the protein, named LolB, were identical to those of HemM, which has been suggested to play a role in 5-aminolevulinic acid synthesis in the cytosol. In contrast to this suggested role, the deduced amino acid sequence of HemM implied that the gene encodes a novel outer membrane lipoprotein. Indeed, an antibody raised against highly purified LolB revealed its outer membrane localization, and inhibited in vitro Lpp incorporation into the outer membrane. Furthermore, LolB was found to be synthesized as a precursor with a signal sequence and then processed to a lipid-modified mature form. An E.coli strain possessing chromosomal hemM under the control of the lac promoter-operator required IPTG for growth, indicating that hemM (lolB) is an essential gene. Outer membrane prepared from LolB-depleted cells did not incorporate Lpp. When the Lpp-LolA complex was incubated with a water-soluble LolB derivative, Lpp was transferred from LolA to LolB. Based on these results, the outer membrane localization pathway for E.coli lipoprotein is discussed with respect to the functions of LolA and LolB.

Links

PubMed PMC1170298 Online version:10.1093/emboj/16.23.6947

Keywords

Amino Acid Sequence; Bacterial Outer Membrane Proteins/genetics; Bacterial Outer Membrane Proteins/metabolism; Biological Transport; Carrier Proteins/genetics; Carrier Proteins/metabolism; Cell Compartmentation; Cell Membrane/metabolism; Escherichia coli/metabolism; Escherichia coli Proteins; Lipoproteins/metabolism; Models, Biological; Molecular Sequence Data; Protein Binding

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ECOLI:LOLB

acts_upstream_of_or_within

GO:0044874: lipoprotein localization to outer membrane

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:LOLB

located_in

GO:0030288: outer membrane-bounded periplasmic space

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ECOLI:LOLB

located_in

GO:0036406: anchored component of periplasmic side of cell outer membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ECOLI:LOLB

acts_upstream_of_or_within

GO:0042157: lipoprotein metabolic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:LOLB

involved_in

GO:0010876: lipid localization

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:LOLB

part_of

GO:0009279: cell outer membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ECOLI:LOLB

involved_in

GO:0010876: lipid localization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:LOLB

involved_in

GO:0042157: lipoprotein metabolic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:LOLB

GO:0089705: protein localization to outer membrane

ECO:0000314:

P

Fig 4; panels C and D

complete
CACAO 10231

ECOLI:LOLB

GO:0044874: lipoprotein localization to outer membrane

ECO:0000314:

P

Fig 4; panels C and D

complete
CACAO 10368

ECOLI:LOLB

involved_in

GO:0051668: localization within membrane

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:LOLB

involved_in

GO:0051668: localization within membrane

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:LOLB

enables

GO:0071723: lipopeptide binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ECOLI:LOLB

involved_in

GO:0044874: lipoprotein localization to outer membrane

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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